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17,828 grants matching “crispr”
Hematopoietic Stem and Progenitor Cell Expansion
$291,433Andre Larochelle · National Heart, Lung, And Blood Institute · ZIA · FY2021 · HL
An instrument-free malaria diagnostic
$291,139Gregory John Tobin · Biological Mimetics, Inc. · R43 · FY2025 · AI
Trajectory and Architecture of Microenvironment-Mediated Resistance in AMLÂ
$291,065Anupriya Agarwal · Oregon Health & Science University · U54 · FY2024 · CA
Trajectory and Architecture of Tumor Intrinsic Drug Resistance in AML
$291,003Jeffrey Wallace Tyner · Oregon Health & Science University · U54 · FY2024 · CA
Genome-scale genetic perturbation of the APOE2 and APOE4 human interactomes
$291,000Patrick Hsu · Salk Institute For Biological Studies · R21 · FY2017 · AG
Using CRISPR-Cas9 technology to develop a mutant cell library for heparan sulfate structure-function study
$291,000Lianchun Wang · University Of Georgia · R21 · FY2016 · HL
Using CRISPR-Cas9 technology to develop a mutant cell library for heparan sulfate structure-function study
$291,000Lianchun Wang · University Of Georgia · R21 · FY2015 · HL
Enzymatic Site-Specific Labeling of RNA for Affinity Isolation
$290,973Neal Krishna Devaraj · University Of California, San Diego · R01 · FY2020 · GM
Bacterial Genetics Core D
$290,926Jeremy Michael Rock · Brigham And Women'S Hospital · U19 · FY2025 · AI
Study of M. tuberculosis under human host selection to identify virulence and barrier lipids (Project 1)
$290,926David Branch Moody · Brigham And Women'S Hospital · U19 · FY2025 · AI
Animal Model Core E
$290,926Clare Margaret Smith · Brigham And Women'S Hospital · U19 · FY2025 · AI
Profiling and Mapping Core
$290,925David Branch Moody · Brigham And Women'S Hospital · U19 · FY2025 · AI
Metabolic determinants of barrier function in rifampin-sensitive and -resistant Mtb
$290,925Kyu Y Rhee · Brigham And Women'S Hospital · U19 · FY2025 · AI
RUI: Collaborative Research: Enzymology of Bacterial Nicotinic Acid Catabolism
$290,796Katherine Hicks · Suny College At Cortland · · FY2018 · BIO
FSHD Drug Discovery
$290,472Charles P Emerson · Univ Of Massachusetts Med Sch Worcester · P50 · FY2022 · HD
Dissecting Persistent Cell Populations in Rhabdomyoscaroma
$290,017Anand G Patel · St. Jude Children'S Research Hospital · K08 · FY2025 · CA
THE USAGE OF CRISPR-CAS HAS MADE TREMENDOUS ADVANCEMENTS IN CROP IMPROVEMENT. THE REASON BEHIND ITS SUCCESS IS DUE TO FLEXIBILITY AND EASY-TO-USE APPLICATION. FURTHERMORE, CRISPR-CAS CAN BE DELIVERED INTO PLANT CELLS WITHOUT TRANSGENE INTRODUCTION, ALLEVIATING A PUBLIC CONCERN. THIS TRANSGENE-FREE STRATEGY IS ACHIEVED BY ADDING ITS COMPONENTS THROUGH PARTICLE BOMBARDMENT AND TISSUE CULTURE PROTOCOLS FOR A CROP OF INTEREST. HOWEVER, MOST PROMISING CUTTING-EDGE LABORATORIES LACK THE TISSUE CULTURE EXPERTISE NECESSARY TO GENERATE GENE-EDITED TROPICAL CROPS DUE TO THEIR COMPLEXITY.THE HAWAI'I AGRICULTURE RESEARCH CENTER (HARC), A NON-PROFIT AGRICULTURE RESEARCH CENTER IN HAWAI'I, HAS VAST EXPERIENCE IN TROPICAL CROP TISSUE CULTURE AND GENETIC ENGINEERING PROTOCOL DEVELOPMENT. TAKING ADVANTAGE OF THE EASINESS OF DELIVERING THE CRISPR-CAS MODULE INTO PLANT CELLS AND HARC'S EXTENSIVE EXPERIENCE IN TROPICAL CROP TISSUE CULTURE,WE WILL DEVELOP A TRANSGENE-FREE GENE-EDITING PLATFORMFOR TROPICAL CROPS.WE WILL IMPLEMENT OUR PLATFORM ON SUGARCANE AND PAPAYA BECAUSE (1) BOTH CROPS HAVE THEIR GENOMES PUBLICLY AVAILABLE, (2) HARC HAS A RICH HISTORY WITH BOTH CROPS IN PLANT BREEDING AND GENETIC ENGINEERING, RESPECTIVELY, AND (3) HARC HAS EXTENSIVE EXPERIENCE IN REGENERATING SUGARCANE AND PAPAYA FROM TISSUE CULTURE - A CRITICAL COMPONENT FOR THE PRODUCTION OF TRANSGENE-FREE GENE-EDITED PLANTS. WE WILL TARGET THEPHYTOENE DESATURASE (PDS)GENE IN BOTH CROPS AS A PROOF-OF-CONCEPT TO VALIDATE OUR PLATFORM. THE DISRUPTION OF THIS GENE WILL LEAD TO WHITE PLANTS, MAKING IT EASY TO DETERMINE GENE-EDITED PLANTS VISUALLY. THIS APPROACH WILL FURTHER OPTIMIZE THE PROTOCOL LEADING TO THE DEVELOPMENT OF ADDED-VALUE TRAITS IN TROPICAL CROPS.WE WILL USE INTERDISCIPLINARY APPROACHES TO DEVELOP THE TRANSGENE-FREE GENE-EDITING PROTOCOL, SUCH AS GENOME SEQUENCE ANALYSIS, MOLECULAR BIOLOGY ASSAYS, AND ESTABLISHED TISSUE CULTURE PROTOCOLS. OUR PROPOSED RESEARCH LEVERAGES HARC'S EXTENSIVE EXPERIENCE IN TROPICAL CROP TISSUE CULTURE AND AN ON-SITE RESEARCH FARM AND GROWTH CHAMBERS FOR FURTHER CROP EVALUATION. OUR STRATEGY FOCUSES ON DEVELOPING TRANSFORMATION METHODS THAT DO NOT REQUIRE THE USE OF PLANT PEST SEQUENCES IN PLANTS OR THE DEVELOPMENT OF GENE-EDITING TECHNOLOGIES THAT DO NOT REQUIRE TRANSFORMATION, THUS FULFILLING AN AFRI CROSSCUTTING PROGRAM PRIORITY.AT THE END OF THIS PROJECT, WE WILL:DETERMINE A STRATEGY TO EDIT A GENETICALLY COMPLEX CROP (SUGARCANE),IDENTIFY WHICH TRANSGENE-FREE DELIVERY STRATEGY IS MOST EFFICIENT IN DEVELOPING GENE-EDITED PLANTLETS, ANDPROVIDE AN EFFICIENT MOLECULAR SCREENING PROTOCOL TO IDENTIFY GENE-EDITED PLANTS WITHOUT SELECTING MARKERS IN TISSUE CULTURE.COMPLETING THE PROJECT WILL SET THE FOUNDATION FOR NEW GENE-EDITING AND TISSUE CULTURE PROTOCOLS FOR OTHER AGRONOMICAL CRITICAL TROPICAL CROPS. IN THELONG TERM, OUR WORK EXPECTS TO PRESENT A STRATEGY TO PRECISELY MANIPULATE TROPICAL CROP GENOMES WITHOUT TRANSGENES. THIS PROJECT WILL ALLOW FACILITATING CROP IMPROVEMENT WHILE ADDRESSING PUBLIC CONCERNS.
$290,000Hawaii Agriculture Research Center · · FY2021 · National Institute of Food and Agriculture
CARROT (DAUCUS CAROTA) IS A ROOT VEGETABLE OFTEN CLAIMED TO BE THE PERFECT HEALTH FOOD. IT IS TASTY AND HIGHLY NUTRITIOUS, CONTAINING HIGH LEVELS OF Β-CAROTENE, FIBER, POTASSIUM, ANTIOXIDANTS, VITAMIN K1 AND VITAMIN B6. CARROTS ARE FOUND IN DIFFERENT COLORS, SUCH AS YELLOW, WHITE, ORANGE, RED AND PURPLE. CARROTS ARE AMONG THE TOP 10 VEGETABLES, BASED ON GLOBAL PRODUCTION RECORDS OF PRIMARY VEGETABLES BASED ON FAO 2017 (HTTP://WWW.FAO.ORG/STATISTICS). INTERESTINGLY, CARROT PRODUCTION PER CAPITA IN THE AMERICAS IS ONLY A THIRD AND A QUARTER TO THAT OF OCEANIA AND EUROPE, RESPECTIVELY. IT SUGGESTS AMPLE ROOM FOR PRODUCTION IMPROVEMENT AS AMERICANS ARE PROJECTED TO EAT A HEALTHIER DIET, RICH IN NUTRITIOUS VEGETABLES. CARROT IS A RELATIVELY RECENTLY DOMESTICATED VEGETABLE CROP. RECENT SEQUENCING OF A CARROT REFERENCE GENOME HAS PAVED THE WAY FOR GENETIC IMPROVEMENTS OF CARROT GERMPLASMS. AS A PROMISING BREEDING TOOL, GENE EDITING IS POISED TO REVOLUTIONIZE CARROT BREEDING. CONSEQUENTLY, THE DEVELOPMENT OF ROBUST CARROT GENOME EDITING PLATFORMS HAS BECOME URGENT. HENCE, IT IS IMPORTANT TO DEVELOP A NEW CARROT GENOME EDITING PLATFORM THAT IS TRANSGENE-FREE. IT WILL NOT ONLY MITIGATE THE REGULATORY BURDEN, BUT ALSO MAKE IT MORE APPEALING TO CONSUMERS AS WE ANTICIPATE THAT GENOME EDITING WILL HELP ACCELERATE THE DEVELOPMENT OF CARROT GERMPLASMS THAT BENEFIT CONSUMERS. WHILE THE GENOME EDITING TECHNOLOGY IS PROMISED TO ACCELERATE CROP BREEDING, ITS DEMONSTRATION AND OPTIMIZATION ARE LAGGING IN BREEDING PROGRAMS IN THE PUBLIC SECTOR. THIS IS ESPECIALLY TRUE FOR AGRICULTURALLY IMPORTANT MINOR CROPS SUCH AS CARROT. IN THIS PROJECT, A TRANSGENE-FREE GENOME EDITING PLATFORM WILL BE ESTABLISHED IN CARROT. BY APPLYING HIGHLY EFFICIENT CRISPR-CAS12A TOOLS TO CARROT THROUGH A TRANSGENE-FREE RNP DELIVERY METHOD, TWO COMPLEMENTARY GENOME EDITING SYSTEMS WILL BE TESTED AND ESTABLISHED IN CARROTS, ONE FOR HIGH-EFFICIENCY TARGETED MUTAGENESIS AND THE OTHER FOR PRECISE GENE REPLACEMENT. GIVEN THE BROAD APPLICABILITY OF THE GENOME EDITING SYSTEMS TO BE DEVELOPED, THE TECHNOLOGIES TO BE DEVELOPED IN THIS PROJECT ARE POTENTIALLY TRANSFORMATIVE AS THEY CAN BE APPLIED TO MANY OTHER MINOR CROPS. THIS PROJECT SPECIFICALLY ADDRESSES THE FOLLOWING TWO CRITERIA OF THE PROGRAM: 1) TRANSFORMATION METHODS THAT DO NOT REQUIRE THE USE OF PLANT PEST SEQUENCES IN PLANTS OR THE DEVELOPMENT OF GENE EDITING TECHNOLOGIES THAT DO NOT REQUIRE TRANSFORMATION; 2) IF TRANSFORMATION TECHNOLOGIES NEED TO BE DEVELOPED, THEY MUST BE COUPLED WITH THE DEVELOPMENT OF GENE EDITING TECHNOLOGIES. BY DEMONSTRATING THESE NEW TRANSGENE-FREE GENOMES EDITING PLATFORMS AND SHARING THE TOOLS AND PROTOCOLS WIDELY TO OTHER RESEARCHERS AND BREEDERS, THE SUCCESS OF THIS PROJECT WILL ENCOURAGE ADOPTION OF THESE BREEDING TECHNOLOGIES IN PUBLIC BREEDING SECTOR, SO THAT THE GOAL SET BY USDA TO INCREASE US AGRICULTURE PRODUCTIVITY BY 40% WHILE CUTTING THE ENVIRONMENTAL FOOTPRINT IN HALF BY 2050 CAN BE MET.
$290,000University Of Maryland, College Park · · FY2021 · National Institute of Food and Agriculture
FSHD Drug Discovery
$289,801Charles P Emerson · Univ Of Massachusetts Med Sch Worcester · P50 · FY2021 · HD
FSHD Drug Discovery
$289,801Charles P Emerson · Univ Of Massachusetts Med Sch Worcester · P50 · FY2020 · HD
Structure and mechanism of CRISPR interference.
$289,649Ailong Ke · Cornell University · R01 · FY2014 · GM
Structure and mechanism of CRISPR interference.
$289,649Ailong Ke · Cornell University · R01 · FY2015 · GM
Mechanisms and Therapeutic Targeting of CD4 Down regulation in African Green Monkeys
$289,535Joseph Christopher Mudd · Tulane University Of Louisiana · R21 · FY2021 · OD
CRISPR tools to dissect the coding and non-coding genome
$289,492Joana Vidigal · Division Of Basic Sciences - Nci · ZIA · FY2024 · CA
Overcoming chemoresistance in triple negative breast cancer
$289,442Ozgur Sahin · Medical University Of South Carolina · R01 · FY2022 · CA