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17,828 grants matching “crispr”
Multiplexed CRISPR-based immune cell RNA profiling by flow and mass cytometry
$299,978Mitchell O'Connell · Dahlia Biosciences, Inc. · R41 · FY2021 · AI
THE CRISPR/CAS NUCLEASES HAVE BEEN USED FOR EFFICIENT TARGETED GENOME EDITING IN VARIOUS ORGANISMS INCLUDING PLANTS. MOST PUBLISHED REPORTS USE AGROBACTERIUM-MEDIATED TRANSGENIC APPROACH TO INTRODUCE GENOME EDITING COMPONENTS INTO PLANTS. PRODUCTION OF TRANSGENIC PLANTS IS TIME CONSUMING, LABOR-INTENSIVE, AND SOME IMPORTANT CROP PLANTS ARE RECALCITRANT TO TRANSFORMATION. THEREFORE, AN EFFICIENT SYSTEM TO DELIVER GENE EDITING COMPONENTS INTO PLANT CELLS THAT CIRCUMVENTS THE REQUIREMENT OF TRANSFORMATION IS REQUIRED. IN OBJECTIVE 1, WE WILL ENGINEER AN RNA VIRUS-BASED TOBACCO RATTLE VIRUS (TRV) VECTORS FOR DELIVERY OF GENE EDITING COMPONENTS INTO THE GROWING PARTS OF THE PLANTS TO INDUCE HIGH EFFICIENCY OF HERITABLE GENE EDITING. SINCE RNA VIRUSES DO NOT INTEGRATE INTO THE PLANT GENOME, THEY ARE NOT TRANSMITTED THROUGH GERMLINE TO THE NEXT GENERATION AND THEREFORE, THE INDUCED MUTATION WILL BE FREE OF TRANSGENES. IN OBJECTIVE 2, WE WILL USE THE OPTIMIZED TRV VECTOR DEVELOPED IN OBJECTIVE 1 TO DELIVER GENE EDITING COMPONENTS AND ESTABLISH THE EFFICIENCY OF GERMINAL TRANSMISSION OF MUTATIONS USING NICOTIANA BENTHAMIANA AND TOMATO PLANT MODEL SYSTEMS.CONSIDERING DELIVERY OF GENOME EDITING COMPONENTS IS A MAJOR BARRIER IN TERMS OF EFFECTIVE USE OF CRISPR TECHNOLOGY IN MANY MAJOR AND MINOR CROP PLANTS, THE SUCCESSFUL DEVELOPMENT OF TRV-BASED SYSTEM WILL HAVE A MAJOR IMPACT ON BREEDING AND CROP IMPROVEMENT PROGRAMS. FURTHERMORE, THE ENGINEERING PRINCIPLES ADAPTED FOR DEVELOPING TRV SYSTEM WILL BE USEFUL TO DEVELOP OTHER VIRUS-BASED VECTORS.
$299,976University Of California, Davis · · FY2020 · National Institute of Food and Agriculture
EAGER: Biomanufacturing: Development of a Quantitative Framework of Directed Stem Cell Differentiation in Scalable Bioreactors
$299,973Emmanuel S Tzanakakis · Tufts University · · FY2015 · ENG
GENOME EDITING IS A REVOLUTIONARY TECHNOLOGY THAT ALLOW ONE TO MAKE PRECISE CHANGES IN A PLANT'S GENETIC BLUEPRINT FOR AGRICULTURE. HOWEVER, ITS APPLICATION TO MANY MINOR CROPS LIKE ALFALFA, GRAPEVINES, LEMONS, LIMES AND ORANGES, IS LIMITED. THE LIMITATION IN USING GENE-EDITING TECHNIQUES IN THESE CROPS IS MAINLY DUE TO THE INABILITY OF STEM CELLS OF THESE PLANTS TO REFORM WHOLE PLANTS IN TISSUE CULTURE. THERE ARE ONLY A VERY FEW VARIETIES OF ALFALFA, ORANGES, LIMES, LEMONS AND GRAPEVINES FOR WHICH WE ARE CAPABLE OF FORMING WHOLE PLANTS FROM CELLS IN TISSUE CULTURE AND THE FEW VARIETIES THAT WE HAVE BEEN SUCCESSFUL WITH DO SO AT A VERY LOW FREQUENCY.WE RECENTLY DISCOVERED THAT A GENE SEQUENCE ENCODING A CHIMERIC PROTEIN THAT COMPRISES A GROWTH REGULATING FACTOR (GRF) AND ITS COFACTOR GRF INTERACTING FACTOR (GIF) (HENCEFORTH GRF-GIF) DRAMATICALLY INCREASED THE ABILITY OF WHEAT STEM CELLS TO REFORM WHOLE PLANTS IN TISSUE CULTURE. WHEN COMBINED WITH THE GENE EDITING SYSTEM CRISPR-CAS9, WE FOUND THAT THIS TECHNOLOGY ALSO SIGNIFICANTLY INCREASES THE TOTAL NUMBER OF GENE-EDITED PLANTS WE CAN PRODUCE USING TISSUE CULTURE. A NOVEL FEATURE OF THIS TECHNOLOGY IS THAT ALTHOUGH THE CHIMERIC GENE ENHANCES REGENERATION IN YOUNG CELLS, THE CHIMERIC GENE IS TURNED OFF IN OLDER TISSUE SO THAT THE PLANT GROWS NORMALLY TO MATURITY. IN WHEAT, THE TECHNOLOGY ALSO ALLOWS US TO MAKE GENE EDITS IN PLANTS WITHOUT THE USE OF A PLANT SELECTABLE MARKER GENE. THE GRF-GIF SYSTEM STIMULATES THE CELLS THAT CONTAIN IT TO REFORM PLANTS ON TISSUE CULTURE PLATES THAT LACK CERTAIN GROWTH FACTORS NORMALLY ADDED TO THE CULTURE MEDIUM AND WHICH ARE REQUIRED FOR THE CELLS TO REFORM PLANTS IN A PETRI DISH. PLANT SELECTABLE MARKER GENES ARE USED TO ALLOW PLANT CELLS TO GROW ON COMPOUND ON WHICH THEY NORMALLY CANNOT GROW. THESE SELECTIVE AGENTS ALLOWS ONLY CELLS CONTAINING THE NEW GENE EDIT ALONG WITH THE PLANT SELECTABLE MARKER GENE TO REFORM WHOLE PLANTS IN THE PRESENCE OF THE SELECTIVE AGENT. PLANT SELECTABLE MARKER GENES CURRENTLY USED ARE OFTEN THOSE THAT CONFER RESISTANT TO ANTIBIOTICS OR HERBICIDES. ALTHOUGH REGULATORY AGENCIES HAVE APPROVED GENETICALLY MODIFIED PLANTS CONTAINING THESE GENES FOR COMMERCIALIZATION, THE PUBLIC WOULD PREFER NOT TO HAVE THESE GENES IN THEIR FOOD SUPPLY.PROMISING PRELIMINARY RESULTS IN CITRUS SUGGEST THAT THIS TECHNOLOGY MAY BE APPLICABLE TO MULTIPLE PLANT SPECIES. THEREFORE THE OVERALL GOAL OF THIS PROPOSAL IS TO USE THIS TECHNOLOGY TO EXPAND THE BENEFITS OF GENE EDITING TO MINOR CROPS BY INCREASE THE EFFICIENCY WITH WHICH PLANT CELLS REFORM PLANTS ACROSS MULTIPLE VARIETIES OF ALFALFA, CITRUS AND VITIS VINIFERA. IN ADDITION, WE WANT TO USE THE ABILITY OF THE GRF-GIF TECHNOLOGY TO REPLACE ANTIBIOTIC AND HERBICIDE PLANT CELL SELECTION SYSTEM. LASTLY, WE WANT TO COMBINE GRF-GIF TECHNOLOGY WITH GENOME EDITING FOR EACH OF THE CROP TO ENHANCE THE PRODUCTION OF GENE-EDITED PLANTS. THIS PROJECT WILL OPEN THE DOOR TO MULTIPLE MINOR CROPS TO BENEFIT FROM THE POWER OF GENOME EDITING TECHNOLOGIES
$299,963University Of California, Davis · · FY2020 · National Institute of Food and Agriculture
Conditional male lethal Anopheles stephensi line for the efficient manufacture of malaria vaccines
$299,951Peter F. Billingsley · Sanaria, Inc. · R43 · FY2024 · AI
CRISPR-based Tropical Infection Diagnostics
$299,937Dmitry Zhukov · Chromologic, Llc · N43 · FY2023 · AI
Functional Genomics
$299,914Aniruddha J. Deshpande · Sanford Burnham Prebys Medical Discovery Institute · P30 · FY2024 · CA
CRISPR-based Diagnostic for Parasites
$299,820Gregory Tobin · Biological Mimetics, Inc. · N43 · FY2023 · AI
A Biosinpired Genetically-Expressible Dual-Modality Peptide-Based Probe for Super-Resolution Correlative Light and Electron Microscopy
$299,812Kevin Michael Lewis · Photon Biosciences, Llc · R41 · FY2024 · GM
AS ONE OF THE MOST POWERFUL GENE-EDITING TOOLS, CRISPR/CAS SYSTEM HAS GREAT POTENTIAL TO SPEED UP THE DEVELOPMENT OF IMPROVED CROP VARIETIES. HOWEVER, ONE OF THE MAJOR BARRIERS FOR EMPLOYING THE TECHNOLOGY IS THE DELIVERY EFFICIENCY. THE COMMONLY USED DELIVERY METHODS IN ANIMAL CELLS, SUCH AS TRANSFECTION AND MICROINJECTION, DO NOT WORK FOR PLANT CELLS DUE TO THE RIGID PLANT CELL WALL. IT IS UNCLEAR WHAT MOLECULAR STRUCTURES MAY HELP DELIVER GENE AND PROTEIN EFFICIENTLY TO PLANT CELLS, AS NO PRIOR SYSTEMATIC STUDIES HAVE BEEN REPORTED ON DEVELOPING DELIVERY AGENTS IN PLANT CELLS. HERE WE PROPOSE TO INVESTIGATE THE DELIVERY AGENTS OF CRISPR PROTEIN AND SGRNA USING THE COMBINATORIAL CHEMISTRY AND MATERIAL FABRICATION METHOD. FIRST, LIBRARIES OF LIPID-LIKE AND BIODEGRADABLE POLYMERIC AGENTS, WHICH HAVE BEEN PROVEN SAFE AND EFFICIENT IN ANIMAL MODELS, WILL BE DESIGNED AND SYNTHESIZED. THEN THE LIBRARY MATERIALS WILL BE ASSEMBLED WITH COMMERCIAL MICRON-SIZED AU PARTICLES THROUGH ADVANCED MATERIAL FABRICATIONS FOR BIOLISTIC DELIVERY. FINALLY, WE FURTHER PROPOSE TO DEVELOP A NEW IN PLANTA PROTEIN DELIVERY SYSTEM USING THE NEWLY DESIGNED LIBRARIES AND ULTRA-SMALL NANOPARTICLES (4 TO 10 NM) WITHOUT THE GENE GUN DEVICE. THE LIBRARY MATERIALS WILL BE EVALUATED IN TERMS OF TOXICITY AND IMPROVED DELIVERY OF MARKER PROTEINS AND CRISPR/CAS9 REAGENTS. THE ULTIMATE GOAL IS TO DEVELOP AN EFFECTIVE SYSTEM FOR THE DELIVERY OF CAS9/GRNA AS RIBONUCLOPROTEINS TO PLANT TISSUES.
$299,668Iowa State University Of Science And Technology · · FY2019 · National Institute of Food and Agriculture
CRISPR-Based in Vitro Diagnostic Device for Rapid Detection of Eukaryotic Pathogens
$299,610Andrew Silverman · Triton Systems, Inc. · N43 · FY2023 · AI
DNA Processing Enzymes with [4Fe4S] Clusters for DNA Signaling
$299,412Jacqueline K Barton · California Institute Of Technology · R01 · FY2016 · GM
DNA Processing Enzymes with [4Fe4S] Clusters for DNA Signaling
$299,412Jacqueline K Barton · California Institute Of Technology · R01 · FY2017 · GM
Molecular Dynamics of Glioma Formation and DNA Damage Response by Mutant IDH1
$299,403Diana Shi · Dana-Farber Cancer Inst · K08 · FY2025 · CA
Molecular Dynamics of Glioma Formation and DNA Damage Response by Mutant IDH1
$299,403Diana Shi · Dana-Farber Cancer Inst · K08 · FY2024 · CA
Integrating epigenomic maps to predict regulatory functions of genetic variants
$299,400Chunyu Liu · University Of Illinois At Chicago · R01 · FY2015 · ES
Role of peroxisome-mitochondrion communication in tissue aging
$299,252Hua Bai · Iowa State University · R01 · FY2025 · AG
Regulation of cell death and disease by a novel membrane protein MADMAN.
$299,167Zhigao Wang · University Of South Florida · R01 · FY2022 · GM
Sorting live cells using RNA-targeting CRISPR-Cas9 (RCas9)
$299,128Mitchell O'Connell · Dahlia Biosciences, Inc. · R41 · FY2020 · HG
A genetic model for metazoan programmed DNA elimination
$299,125Jianbin Wang · University Of Tennessee Knoxville · R01 · FY2023 · GM
Lipid droplet regulation and proteome dynamics
$299,110James A Olzmann · University Of California Berkeley · R01 · FY2024 · DK
EAGER: Clonal propagation of tomato through seeds
$299,071Venkatesan Sundaresan · University Of California-Davis · · FY2019 · BIO
A genetic model for metazoan programmed DNA elimination
$298,988Jianbin Wang · University Of Tennessee Knoxville · R01 · FY2024 · GM
A genetic model for metazoan programmed DNA elimination
$298,847Jianbin Wang · University Of Tennessee Knoxville · R01 · FY2025 · GM
Development of AAV-AXN-007 gene therapy to treat glaucoma.
$298,819Shane Hegarty · Axonis Therapeutics, Inc. · R43 · FY2022 · EY