AS ONE OF THE MOST POWERFUL GENE-EDITING TOOLS, CRISPR/CAS SYSTEM HAS GREAT POTENTIAL TO SPEED UP THE DEVELOPMENT OF IMPROVED CROP VARIETIES. HOWEVER, ONE OF THE MAJOR BARRIERS FOR EMPLOYING THE TECHNOLOGY IS THE DELIVERY EFFICIENCY. THE COMMONLY USED DELIVERY METHODS IN ANIMAL CELLS, SUCH AS TRANSFECTION AND MICROINJECTION, DO NOT WORK FOR PLANT CELLS DUE TO THE RIGID PLANT CELL WALL. IT IS UNCLEAR WHAT MOLECULAR STRUCTURES MAY HELP DELIVER GENE AND PROTEIN EFFICIENTLY TO PLANT CELLS, AS NO PRIOR SYSTEMATIC STUDIES HAVE BEEN REPORTED ON DEVELOPING DELIVERY AGENTS IN PLANT CELLS. HERE WE PROPOSE TO INVESTIGATE THE DELIVERY AGENTS OF CRISPR PROTEIN AND SGRNA USING THE COMBINATORIAL CHEMISTRY AND MATERIAL FABRICATION METHOD. FIRST, LIBRARIES OF LIPID-LIKE AND BIODEGRADABLE POLYMERIC AGENTS, WHICH HAVE BEEN PROVEN SAFE AND EFFICIENT IN ANIMAL MODELS, WILL BE DESIGNED AND SYNTHESIZED. THEN THE LIBRARY MATERIALS WILL BE ASSEMBLED WITH COMMERCIAL MICRON-SIZED AU PARTICLES THROUGH ADVANCED MATERIAL FABRICATIONS FOR BIOLISTIC DELIVERY. FINALLY, WE FURTHER PROPOSE TO DEVELOP A NEW IN PLANTA PROTEIN DELIVERY SYSTEM USING THE NEWLY DESIGNED LIBRARIES AND ULTRA-SMALL NANOPARTICLES (4 TO 10 NM) WITHOUT THE GENE GUN DEVICE. THE LIBRARY MATERIALS WILL BE EVALUATED IN TERMS OF TOXICITY AND IMPROVED DELIVERY OF MARKER PROTEINS AND CRISPR/CAS9 REAGENTS. THE ULTIMATE GOAL IS TO DEVELOP AN EFFECTIVE SYSTEM FOR THE DELIVERY OF CAS9/GRNA AS RIBONUCLOPROTEINS TO PLANT TISSUES.