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17,828 grants matching “crispr”
HIV-CRISPR: A novel approach to the comprehensive discovery of HIV latency factors
$880,000Michael Emerman · Fred Hutchinson Cancer Research Center · DP1 · FY2021 · DA
HIV-CRISPR: A novel approach to the comprehensive discovery of HIV latency factors
$880,000Michael Emerman · Fred Hutchinson Cancer Research Center · DP1 · FY2020 · DA
Gene Function, Expression and Regulation in Zebrafish
$879,907Benjamin Feldman · Eunice Kennedy Shriver National Institute Of Child Health & Human Development · ZIC · FY2019 · HD
Connecting TDP-43 Pathology to the Molecular Profiles of Neurodegeneration
$879,816Molly Gale Hammell · New York University School Of Medicine · R01 · FY2024 · NS
Integrative approaches to elucidate p53 transcriptional networks during carcinogenesis
$879,804Laura D Attardi · Stanford University · R35 · FY2024 · CA
Short-term and long-term effects of methamphetamine exposure on residual viral transcription during treated HIV disease
$879,803Sulggi Angela Lee · University Of California, San Francisco · R33 · FY2024 · DA
Collaborative Research: Molecular Mechanisms Underlying Repeated Evolution: Integrating Micro- and Macroevolutionary Analyses and Functional Genomics
$879,124Johanna E Kowalko · Lehigh University · · FY2023 · BIO
In vivo CRISPR engineering of B cells to produce anti-HIV broadly neutralizing antibodies using novel nanoparticles
$877,927Jennifer Eileen Adair · Fred Hutchinson Cancer Center · R01 · FY2022 · AI
Modeling neurofibromatosis-1 disease heterogeneity to optimize risk assessment and treatment
$877,001David H. Gutmann · Washington University · R35 · FY2025 · NS
A community resource for germline and somatic genetic disease modeling in zebrafish
$876,458Calum A. Macrae · Brigham And Women'S Hospital · R24 · FY2025 · OD
A community resource for germline and somatic genetic disease modeling in zebrafish
$876,458Calum A. Macrae · Brigham And Women'S Hospital · R24 · FY2024 · OD
Modeling KRAS-Dependent Synthetic Lethality in Human Colon Organoids
$876,328Calvin J Kuo · Stanford University · U01 · FY2018 · CA
Collaborative Research: EDGE FGT: Advancing CRISPR approaches for bacterial functional genomics applied to marine Vibrio-host interactions
$876,272Mark J Mandel · University Of Wisconsin-Madison · · FY2025 · BIO
Chromatin Structure in Regulation of Mammalian Gene Expression
$876,175Ann Dean · National Institute Of Diabetes And Digestive And Kidney Diseases · ZIA · FY2015 · DK
POWDERY MILDEW (PM), A FUNGAL DISEASE, MAINLY CAUSED BY GOLOVINOMYCES AMBROSIAE HAS DEVASTATING IMPACTS ON INDUSTRIAL HEMP THROUGHOUT NORTH AMERICA RESULTING IN SIGNIFICANT CROP LOSS. UNFORTUNATELY, THE MOLECULAR ARCHITECTURE UNDERLYING PM RESISTANCE (PMR) IN HEMP IS ONLY POORLY UNDERSTOOD. THIS LACK OF KNOWLEDGE ABOUT PMR REGULATORS AND THE RESULTING ABSENCE OF RESISTANT GENETIC MATERIAL IS A MAJOR PROBLEM FOR HEMP PRODUCTION IN THE US. THE GOAL OF THE PROPOSED RESEARCH IS TO GENERATE A GENETIC PMR TOOLBOX WHICH WILL ACCELERATE THE DEVELOPMENT OF PM-RESISTANT HEMP STRAINS ALLOWING THE CREATION OF HEMP PLANTS WITH DESIRABLE TRAITS MORE QUICKLY THAN TRADITIONAL BREEDING METHODS. TO ACHIEVE THIS, WE WILL ILLUMINATE THE EARLY INFECTION STAGES OF THE PM-CAUSING FUNGUS GOLOVINOMYCES AMBROSIAE ON PM SUSCEPTIBLE CULTIVARS EMPLOYING VARIOUS STATE-OF-THE-ART GENOMIC TOOLS (RNA-SEQ, ATAC-SEQ) AT TISSUE AND SINGLE NUCLEI LEVEL RESOLUTION. THIS WILL LEAD TOGETHER WITH GENETIC MAPPING OF RESISTANCE GENES AND THE MILDEW LOCUS O FAMILY (MLO) GENE FAMILY TO A CANDIDATE GENE LIST OF PMR REGULATORS WHICH WILL BE DISRUPTED USING CRISPR-CAS9. DISRUPTION OF SUSCEPTIBILITY GENES WILL COMPROMISE THE ABILITY OF G. AMBROSIAE TO SUCCESSFULLY COLONIZE HEMP PLANTS WHEREAS THE CRISPR/CAS9-MEDIATED DELETION OF RESISTANCE GENES WILL CAUSE THE OPPOSITE LEADING TO INCREASED SUSCEPTIBILITY. THE RESULTING GENETIC MATERIAL WILL BE TESTED FOR PMR IN FIELD TRIALS AND PROMISING STRAINS WILL BE RAPIDLY MADE AVAILABLE TO INDUSTRY AND RESEARCH STAKEHOLDERS. THIS RESEARCH, DRIVEN BY GROWER REQUESTS, HAS THE POTENTIAL TO GREATLY BENEFIT THE HEMP INDUSTRY BY IMPROVING CROP PERFORMANCE, MITIGATING CROP LOSS AND REDUCING THE NEED FOR HARMFUL PESTICIDES.
$875,854Rutgers, The State University · · FY2025 · National Institute of Food and Agriculture
Project 1: Transmission Biology of M. tuberculosis: Genes Required to Survive Stressful Transitions
$875,451Carl F Nathan · Weill Medical Coll Of Cornell Univ · P01 · FY2023 · AI
Defining mechanisms of disease and repair in a viral model of multiple sclerosis
$875,447Thomas E Lane · University Of California-Irvine · R35 · FY2022 · NS
Deep mutational scanning of MYH7 in genome-edited cardiomyocytes
$875,082Kai-Chun Daniel Yang · University Of Washington · R01 · FY2025 · HL
Mitochondrial metabolite compartmentalization in health and disease
$875,000Shingo Kajimura · Beth Israel Deaconess Medical Center · DP1 · FY2025 · DK
Mitochondrial metabolite compartmentalization in health and disease
$875,000Shingo Kajimura · Beth Israel Deaconess Medical Center · DP1 · FY2021 · DK
Mitochondrial metabolite compartmentalization in health and disease
$875,000Shingo Kajimura · Beth Israel Deaconess Medical Center · DP1 · FY2023 · DK
Mitochondrial metabolite compartmentalization in health and disease
$875,000Shingo Kajimura · Beth Israel Deaconess Medical Center · DP1 · FY2022 · DK
Dissecting the host-plasmid interplay to unravel the spread of ESBL-producing K. pneumoniae
$874,998Barry Neal Kreiswirth · Hackensack University Medical Center · U01 · FY2025 · AI
In vivo hematopoietic stem cell genetic modification by viral particles
$874,907Daniel Evan Bauer · Boston Children'S Hospital · R01 · FY2025 · HL
Environmental Epigenomics and Precision Environmental Health
$874,519Dana Dolinoy · University Of Michigan At Ann Arbor · R35 · FY2023 · ES