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84,503 grants matching “bioinformatics”
Screen and functional validation of Pediatric Cardiomyopathy genetic variants in Drosophila
$650,351Zhe Han · University Of Maryland Baltimore · R01 · FY2023 · HD
Screen and functional validation of Pediatric Cardiomyopathy genetic variants in Drosophila
$650,351Zhe Han · University Of Maryland Baltimore · R01 · FY2025 · HD
Epigenetic Profiling of circulating cell-free DNA for the Monitoring of Graft-Versus-Host Disease after Hematopoietic Cell Transplantation
$650,319Iwijn De Vlaminck · Cornell University · R01 · FY2024 · AI
Autophagy-dependent exosome loading and biogenesis in AD and FTD
$650,216Eric J Huang · University Of California, San Francisco · R01 · FY2020 · AG
Autophagy-dependent exosome loading and biogenesis in AD and FTD
$650,216Eric J Huang · University Of California, San Francisco · R01 · FY2021 · AG
Lifecourse Exposures & Diet: Epigenetics, Maturation & Metabolic Syndrome
$650,136Karen Eileen Peterson · University Of Michigan At Ann Arbor · P01 · FY2014 · ES
Collaborative Research: AToL: ACCESS DNA viruses: A Comprehensive survey of Circular Eukaryotic Single-Stranded DNA viruses in Invertebrates and Fungi
$650,121Siobain M Duffy · Rutgers University New Brunswick · · FY2012 · BIO
A Collaborative Search for New Genes for Non-Syndromic Deafness
$650,100Mustafa Tekin · University Of Miami School Of Medicine · R01 · FY2017 · DC
The Genetic and Genomic Study of MicroRNA in Bipolar and Schizophrenia
$650,074Chunyu Liu · University Of Chicago · R01 · FY2008 · MH
Metapangenomics of the Oral Microbiome
$650,052Gary G Borisy · Ada Forsyth Institute, Inc. · R01 · FY2021 · DE
ROCHESTER NATHAN SHOCK CENTER
$650,001University Of Rochester · P30 · FY2000 · AG
Genomic Characterization and Surveillance of Microbial Threats in West Africa
$650,000Christian T. Happi · Redeemer'S University · U54 · FY2018 · HG
Institute of Human Virology H3Africa Biorepository (I-HAB)
$650,000Alash'le G. Abimiku · Institute Of Human Virology · U24 · FY2020 · HG
DartCF: The Dartmouth Cystic Fibrosis Research Center
$650,000Dean R Madden · Dartmouth College · P30 · FY2024 · DK
Canine models for characterization, diagnosis, and treatment of human cancers using comparative canine-human transcriptomics
$650,000Gregory Tawa · National Center For Advancing Translational Sciences · ZIA · FY2024 · TR
IMPROVING WHEAT YIELD POTENTIAL TO MEET FUTURE DEMANDS FOR FOOD WHILE REDUCING LAND USE AND PROMOTING SUSTAINABLE FARMING REQUIRES CONCERTED EFFORTS OF RESEARCH AND BREEDING COMMUNITY AIMED AT 1) DISCOVERING GENETIC VARIANTS CONTROLLING PHYSIOLOGICAL AND DEVELOPMENTAL PROCESSES THAT CONTRIBUTE TO TRAITS OF AGRONOMIC IMPORTANCE, ESPECIALLY PRODUCTIVITY AND NUTRITIONAL VALUE TRAITS, 2) EVALUATING THE PHENOTYPIC EFFECTS OF INDIVIDUAL AND STACKED BENEFICIAL VARIANTS IN ADAPTED GERMPLASM UNDER REALISTIC AGRICULTURAL SETTINGS, AND THEN 3) USING THIS KNOWLEDGE TO REDESIGN WHEAT PLANT BY STRATEGICALLY COMBINING THOSE ALLELES WHOSE INTERACTION GREATLY ENHANCES TRAIT EXPRESSION. WHILE PROGRESS WAS MADE TOWARDS IDENTIFYING THE GENETIC BASIS OF MANY AGRONOMIC TRAITS, WE STILL NEED TO EXPAND GENETIC DIVERSITY ACCESSIBLE FOR BREEDING BY EITHER INTRODUCING NEW DIVERSITY FROM THE ANCESTRAL POPULATIONS OF WILD RELATIVES OR LANDRACES, OR BY CREATING NOVEL VARIANTS BY MUTAGENESIS OR GENE EDITING. WE STILL HAVE LIMITED UNDERSTANDING OF HOW DISCOVERED GENES AND THEIR ALLELES WILL PERFORM IN DIVERSE GENETIC BACKGROUNDS OR ENVIRONMENTS, OR HOW TRAITS AFFECTING DIFFERENT ASPECTS OF WHEAT BIOLOGY WILL INTERACT WITH EACH OTHER ONCE COMBINED. TO ADDRESS THESE CHALLENGES, URGENT INTEGRATION OF INNOVATIVE TECHNOLOGIES AND BREEDING STRATEGIES INTO THE TRANSLATIONAL ACTIVITIES IS REQUIRED.OVER THE LAST DECADE, BY THE EFFORTS OF NATIONAL AND INTERNATIONAL WHEAT RESEARCH PROGRAMS, THE GENETIC BASIS OF MANY AGRONOMIC TRAITS HAS BEEN ESTABLISHED. THE RELEASE OF THE ANNOTATED WHEAT GENOME SEQUENCE AND DEVELOPMENT OF COMPARATIVE GENOMICS TOOLS AND RESOURCES PROVIDED A POWERFUL FRAMEWORK FOR EXTRAPOLATING GENE MAPPING INFORMATION FROM OTHER CROPS INTO WHEAT. THESE DISCOVERIES NOW PROVIDE UNIQUE OPPORTUNITY TO REDESIGN BIOLOGICAL PATHWAYS UNDERLYING MAJOR AGRONOMIC TRAITS IN WHEAT BY INTRODUCING THE FAVOURABLE ALLELES OF CAUSAL GENES INTO THE BREEDING PIPELINES.MANY STUDIES DEMONSTRATED THAT THE CRISPR-CAS SYSTEM CAN TAKE FULL ADVANTAGE OF THESE NEW GENOMIC RESOURCES AND FACILITATE THE CHARACTERIZATION AND DEPLOYMENT OF THE NOVEL GENE VARIANTS. OUR PROJECT WILL ESTABLISH PLANT BREEDING PARTNERSHIP INCLUDING KANSAS STATE UNIVERSITY (KSU) AND THE UNIVERSITY OF SASKATCHEWAN (CANADA) WITH THE AIM TO INTEGRATE THE CRISPR-CAS-BASED TECHNOLOGY INTO THE WHEAT PRE-BREEDING PIPELINES AND IMPROVE WHEAT PRODUCTIVITY AND NUTRITIONAL QUALITY TRAITS IN ADAPTED GERMPLASM. THIS PROJECT WILL BUILD ON THE RESOURCES GENERATED IN THE ON-GOING GENE EDITING PROJECTS CONDUCTED BY THE PROJECT DIRECTORS AND COLLABORATORS. THE PROJECT ACTIVITIES WILL BE INTEGRATED WITH THE WHEAT CAP AND IWYP PROJECTS, AND THE FUTURE NIFA IWYP WINTER WHEAT BREEDING INNOVATION HUB IN MANHATTAN (KS).HERE, WE WILL CREATE VARIATION IN THE PROTEIN CODING OR REGULATORY REGIONS OF GENES THAT CAN INFLUENCE PLANT GROWTH, SPIKE AND GRAIN DEVELOPMENT, AND NUTRIENT ACCUMULATION IN GRAIN. WE WILL MAKE USE OF CRISPR-EDITED WHEAT LINES GENERATED BY OUR TEAM IN A PREVIOUS PROJECT WITH MUTATIONS IN THE GENES AFFECTING GRAIN SIZE AND WEIGHT, AND NUMBER OF GRAINS PER HEAD.WE WILL TEST A NEW STRATEGY TO CREATE NOVEL PHENOTYPIC VARIATION BY MUTATING THE REGULATORY REGIONS OF GENES CONTROLLING NITROGEN UPTAKE, CARBON FIXATION, GROWTH AND NUTRIENT REMOBILIZATION. CURRENTLY, WE HAVE LIMITED UNDERSTANDING OF THE RANGE OF POSSIBLE QUANTITATIVE VARIATION FOR THESE TRAITS THAT CAN BE GENERATED BY EDITING THE REGULATORY REGIONS OF GENES. IN THIS PROJECT, WE WILL INVESTIGATE PHENOTYPES PRODUCED BY THE CRISPR-CAS9-EDITED PROMOTER VARIANTS AND ASSESS THEIR UTILITY FOR WHEAT IMPROVEMENT. BY COMBINING AND TESTING DIFFERENT REGULATORY VARIANTS OF GENES SHOWING DIFFERENT LEVELS OF EXPRESSION, WE WILL IDENTIFY OPTIMAL COMBINATIONS OF VARIANTS SUPPORTING EFFECTIVE NITROGEN UPTAKE AND PLANT GROWTH.THE IDENTIFICATION AND ASSEMBLY OF POSITIVELY INTERACTING COMBINATIONS OF GENES AFFECTING WHEAT PRODUCTIVITY TRAITS HAS A POTENTIAL TO SUBSTANTIALLY INCREASE YIELD. ONE OF THE PRE-REQUISITES FOR THE IMPLEMENTATION OF THIS STRATEGY IS THE IDENTIFICATION OF ALLELES THAT DO NOT SHOW NEGATIVE INTERACTION. BY TRANSFERRING THE CRISPR-EDITED GENE VARIANTS INTO THE ADAPTED GERMPLASM ALREADY SELECTED FOR OTHER BENEFICIAL AND COMPLEMENTARY TRAITS (E.G. HIGH BIOMASS, IMPROVED PLANT ARCHITECTURE, ETC.), THIS PROJECT WILL INVESTIGATE THE UTILITY OF QUANTITATIVE TRAIT VARIATION INDUCED BY GENE EDITING FOR WHEAT IMPROVEMENT, AND IDENTIFY THE COMBINATIONS OF GERMPLASM AND CRISPR-CAS INDUCED ALLELES CAPABLE OF SUPPRESSING NEGATIVE INTERACTION AMONG THE WHEAT PRODUCTION TRAITS.WITH GENE EDITING BECOMING ONE OF THE VALUABLE ASSETS IN THE BREEDER'S CROP IMPROVEMENT TOOLBOX, TRAINING OPPORTUNITIES FOR GRADUATE STUDENTS AND POSTDOCTORAL RESEARCHERS THAT INTEGRATE GENE EDITING SYSTEM WITH BREEDING METHODOLOGIES, BIOINFORMATICS, COMPARATIVE GENOMICS, AND MOLECULAR GENETICS ARE REQUIRED.IN OUR PROJECT, WE WILL DEVELOP EDUCATIONAL MODULES FOR POSTDOCTORAL RESEARCHERS AND PHD STUDENTS TO PROVIDE TRAINING IN APPLICATIONS OF GENE EDITING TECHNOLOGY FOR CROP IMPROVEMENT, REGULATORY ASPECTS OF CROP BIOTECHNOLOGY AND SCIENCE COMMUNICATION. THESE TRAINING IS INTENDED TO PREPARE THE NEW GENERATION OF CROP SCIENTISTS TO FACE THE CHALLENGES OF COMMUNICATING THEIR DISCOVERIES TO THE PUBLIC, CONSUMER GROUPS AND POLICYMAKERS TO ENSURE THAT THE SCIENCE-BASED POLICIES PREVAIL.
$650,000Kansas State University · · FY2020 · National Institute of Food and Agriculture
ABCD-USA Consortium: Coordinating Center
$650,000Terry L Jernigan · University Of California, San Diego · U24 · FY2020 · DA
DCCPS Basic and Behavioral Research Network
$650,000David Heimbrook · Leidos Biomedical Research, Inc. · N01 · FY2014 · CA
P30 Core Grant For Vision Research
$650,000Claire H Mitchell · University Of Pennsylvania · P30 · FY2025 · EY
ALTHOUGH PROPER EPIGENETIC MARKS WITHIN THE PREIMPLANTATION EMBRYO ARE ESSENTIAL FOR NORMAL DEVELOPMENT, REGULATION OF EPIGENETIC MODIFICATIONS DURING EARLY EMBRYOGENESIS IS POORLY UNDERSTOOD. OUR PREVIOUS STUDIES PINPOINT THAT THE OOCYTE-SPECIFIC TET FAMILY GENE (TET3L) IS CRITICAL FOR NORMAL FERTILIZATION BY REGULATING EPIGENETIC CHANGES DURING EARLY EMBRYOGENESIS. UNDERSTANDING THE ACTIONS OF THE TET FAMILY DURING EARLY EMBRYOGENESIS WILL ALLOW US TO EXPAND OUR KNOWLEDGE OF HOW EPIGENETIC MARKS ARE REGULATED DURING EMBRYOGENESIS AND USE THE KNOWLEDGE TO DESIGN NOVEL STEM CELL LINES. WE PROPOSE TO IDENTIFY HOW TET3L AFFECTS CELLULAR SPECIFICATION DURING EMBRYOGENESIS, THE UNIQUENESS OF TET3L AMONG DIFFERENT TET FAMILY MEMBERS, AND THE PATHWAY OF REPROGRAMMING INITIATED BY TET3L. WE WILL APPLY ADVANCED MOLECULAR AND BIOINFORMATIC ANALYSIS TECHNOLOGIES TO OBTAIN THE ANSWERS. THROUGH THIS RESEARCH, WE EXPECT TO ELUCIDATE THE IMPACT OF TET3L ON VARIOUS EPIGENETIC MARKS WHICH WILL BE THEFIRST REPORT IN NON-RODENT SPECIES. UNDERSTANDING THE ACTION OF KEY EPIGENETIC REGULATORS, SUCH AS THE TET FAMILY, DURING EMBRYOGENESIS WILL ALLOW US TO IMPROVE EMBRYO VIABILITY IN DOMESTIC SPECIES AND OFFER A NOVEL APPROACH TO GENERATE STEM CELLS FROM SOMATIC CELLS.
$650,000University Of Missouri System · · FY2022 · National Institute of Food and Agriculture
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** DISEASE OUTBREAKS ARE A MAJOR IMPEDIMENT TO RELIABLE AQUACULTURE PRODUCTION. OUR PRIOR AFRI FUNDING HAS LED TO THE DEVELOPMENT, SCIENTIFIC VALIDATION, AND NOVEL FORMULATION OF A PROBIOTIC BACTERIUM,PHAEOBACTER INHIBENSS4, THAT IS NOW COMMERCIALLY AVAILABLE FOR OYSTER LARVICULTURE. S4 TREATMENTS IN HATCHERIES PROTECT AGAINST PROMINENT PATHOGENS, INCLUDINGVIBRIO CORALLIILYTICUSRE22.RECENT RESULTS SUGGEST THAT S4 HAS BROADER PROBIOTIC ACTIVITY AGAINST BACTERIAL AQUACULTURE DISEASES. OUR DATA ALSO REVEAL THAT RE22 KILLS LARVAE USING TWO PREVIOUSLY UNRECOGNIZED VIRULENCE MECHANISMS, TYPE VI AND TYPE III SECRETION SYSTEMS, AND THAT S4 DOWNREGULATES THESE PATHWAYS.OUR GOAL IS TO DEVELOP NOVEL LOW-COST AND 'GREEN' TOOLS FOR AQUACULTURE. THIS PROJECT WILL TEST THE HYPOTHESIS THAT SPECIFIC MOLECULAR MECHANISMS EMPLOYED BY THE PROBIONT AND PATHOGEN DETERMINE THE OUTCOMES OF INTERSPECIES INTERACTIONS AND, ULTIMATELY, THE SURVIVAL OF THE HOST. IN OBJECTIVES 1 AND 2, WE ADDRESS KNOWLEDGE GAPS REGARDING THE MOLECULAR MECHANISMS USED BY THE PATHOGEN RE22 TO KILL HOSTS. USING A GENOMICS AND BIOINFORMATICS DRIVEN APPROACH, WE WILL DISSECT THE TYPE VI AND TYPE III SECRETION SYSTEMS OF RE22 TO REVEAL THE CRITICAL COMPONENTS OF THESE VIRULENCE NANOMACHINES. MUTAGENESIS WILL BE USED TO VERIFY THE ROLES OF SPECIFIC GENES. IN OBJECTIVE 3, WE WILL USE AN INNOVATIVE TRANSCRIPTOMICS DRIVEN APPROACH TO TEST HOW PROBIONT S4 MODULATES PATHOGENESIS PATHWAYS IN OTHER AQUACULTURE PATHOGENS. THIS PROJECT ADDRESSES THE PROGRAM PRIORITY AREAS OF (1) DISEASE PREVENTION, (2) THERAPEUTIC INTERVENTIONS FOR DISEASE REDUCTION, AND (3) CREATING NEW KNOWLEDGE IN THE CELLULAR, MOLECULAR, AND GENOMIC/GENETIC ASPECTS OF HEALTH AND DISEASE.
$650,000University Of Rhode Island · · FY2023 · National Institute of Food and Agriculture
Functional RNA elements in the human genome
$650,000Xiang-Dong Fu · University Of California, San Diego · R01 · FY2009 · HG
KULMAP: Human Kidney, urinary tract and lung mapping center
$650,000Kun Zhang · University Of California, San Diego · U54 · FY2018 · HL
Integrating Data Science and Hands-on Experience into the Community College Biotechnology Classroom with Applications to Antibody Engineering
$650,000Dylan A Bulseco · Institute For Future Intelligence, Inc. · · FY2023 · EDU
Supporting Retention and Graduation of Biology, Chemistry, and Mathematics Majors in Rural Central Louisiana
$650,000Guoyi Ke · Louisiana State University At Alexandria · · FY2021 · EDU