Sort
17,828 grants matching “crispr”
2014 Waterman Award
$1,000,000Feng Zhang · Broad Institute, Inc. · · FY2014 · BIO
EDGE FGT: NSF-BSF: Development of Viral Vectors for Amphibian Gene Delivery and Manipulation
$1,000,000Darcy B Kelley · Columbia University · · FY2021 · BIO
Beginnings: Innovative, Immersive Training Program in Synthetic Biology and Biomanufacturing
$999,850Rana Khan · Hood College · · FY2025 · EDU
THIS PROJECT WILL GENETICALLY IMPROVE SEED SIZE AND SEED OIL CONTENT OF FIELD PENNYCRESS (THLASPI ARVENSEL.; PENNYCRESS) FOR ITS USE AS A NEW WINTER ANNUAL CASH COVER CROP FOR THE U.S. MIDWEST. PENNYCRESS IS A WINTER HARDY COVER CROP THAT PROVIDES ECOSYSTEM SERVICES SUCH AS REDUCED SOIL EROSION AND NUTRIENT LOSS IN BETWEEN FALL CORN HARVEST AND SPRING SOYBEAN PLANTING. UNLIKE TRADITIONAL COVER CROPS, FIELD PENNYCRESS PRODUCES A MATURE OILSEED IN LATE SPRING, ALLOWING FARMERS TO HARVEST TWO CASH CROPS IN ONE YEAR. WILD-DERIVED PENNYCRESS LINES HAVE BEEN SHOWN TO YIELD 1,500 KG/HA ON AVERAGE. PENNYCRESS SEEDS CONTAIN APPROXIMATELY 33% OIL BY WEIGHT, AND THE OIL IS AN EXCELLENT BIOFUELS FEEDSTOCK. AFTER PRESSING, THE REMAINING PENNYCRESS MEAL COULD BE USED AS ANIMAL FEED. HOWEVER, DESPITE THESE ENVIRONMENTAL AND ECONOMIC BENEFITS, PENNYCRESS IS CURRENTLY LIMITED BY ITS SMALL SEED SIZE (1 MG/SEED), WHICH CAN COMPLICATE PLANTING, HARVESTING, AND HANDLING THE SEED. INCREASING PENNYCRESS SEED SIZE WOULD ALSO INCREASE THE EFFICIENCY OF OIL EXTRACTION. IN ADDITION TO IMPROVING THE SEED SIZE, INCREASED OIL CONTENT IN THE SEED WOULD ALSO IMPROVE THE ECONOMICS OF GROWING AND PROCESSING OF PENNYCRESS AS A BIOFUELS FEEDSTOCK.WITH PREVIOUS USDA NIFA FUNDING, WE DEVELOPED SEVERAL EMS-INDUCED PENNYCRESS MUTANT LINES EXHIBITING KEY DOMESTICATION TRAITS SUCH AS REDUCED SEED POD SHATTER, EARLIER FLOWERING, AND IMPROVED FATTY ACID PROFILES. WE HAVE ALSO DEVELOPED AND DEMONSTRATED THE UTILITY OF PENNYCRESS AGROBACTERIUM-MEDIATED PLANT TRANSFORMATION AND CRISPR-CAS9 GENOME EDITING, GENERATING PENNYCRESS LINES WITH UNDETECTABLE LEVELS OF ERUCIC ACID IN SEED OIL. WITH PENNYCRESS DOMESTICATION WELL UNDER WAY, OUR NEW GOAL IS TO IDENTIFY, CHARACTERIZE, AND INTROGRESS INTO BREEDING LINES TRAITS THAT WILL IMPROVE PENNYCRESS EFFICIENCY AND UTILITY AS A BIOFUEL FEEDSTOCK SPECIES AND MAKE THE SEED EASIER FOR PRODUCERS TO HANDLE, NAMELY: 1) INCREASED SEED SIZE AND 2) INCREASED SEED OIL CONTENT. THESE TWO TRAITS WILL BE KEY FOR RAPID COMMERCIALIZATION OF THE SPECIES. IN ADDITION TO IDENTIFYING AND CHARACTERIZING EMS-INDUCED MUTANT LINES WITH INCREASED SEED SIZE AND OIL CONTENT, WE WILL ALSO EVALUATE UNTAPPED GENETIC VARIATION FOR THESE TRAITS IN WILD-DERIVED PENNYCRESS LINES USING QUANTITATIVE TRAIT LOCI (QTL) MAPPING. FINALLY, WE WILL INTROGRESS IDENTIFIED QTLS AND BENEFICIAL MUTATIONS INTO ELITE PENNYCRESS LINES USING MARKER ASSISTED SELECTION. THIS RESEARCH WILL AID IN THE GENERATION OF HIGH-YIELDING, ELITE PENNYCRESS VARIETIES WITH LARGER SEED SIZE AND HIGHER OIL CONTENT. PRODUCERS HAVE EXPRESSED INTEREST IN PLANTING PENNYCRESS AS A CASH COVER CROP, HOWEVER THEY HAVE BEEN CONCERNED ABOUT THE SMALL SEED SIZE. LARGER SEEDED PENNYCRESS VARIETIES WILL BE EASIER TO HANDLE WITH CONVENTIONAL EQUIPMENT AND WILL LEAD TO INCREASED ADOPTION OF PENNYCRESS AS A WINTER OILSEED COVER CROP FOR THE U.S. MIDWEST. INCREASING THE SEED OIL CONTENT WILL GREATLY IMPROVE THE ECONOMICS OF GROWING AND PROCESSING PENNYCRESS. BY INTEGRATING NEW PENNYCRESS VARIETIES INTO THE CORN-SOYBEAN ROTATION SYSTEM, U.S. PRODUCERS CAN INCREASE SUSTAINABILITY BY REDUCING SOIL EROSION, NUTRIENT LOSS, AND HERBICIDE APPLICATIONS. ESTABLISHING PENNYCRESS AS AN OFF-SEASON CROPPING SYSTEM WILL IMPROVE RURAL ECONOMIES BY CREATING NEW JOBS AND DEVELOPING NEW PRODUCTS THAT REPLACE FOSSIL FUELS WITH LOCALLY-PRODUCED BIOFUELS.
$999,842Regents Of The University Of Minnesota · · FY2019 · National Institute of Food and Agriculture
FDT-BioTech: VAScTWIN: Multiscale Digital Twin for Predictive Modeling and Genetic Control of Cardiac Vascular Regeneration
$999,745Aishwarya R Pawar · Iowa State University · · FY2025 · MPS
CCR, LGI, Flow Cytometry Core
$999,466Ferenc Livak · Division Of Basic Sciences - Nci · ZIC · FY2018 · CA
Coordinate Regulation of Virulence in Vibrio Cholerae
$999,436John J Mekalanos · Harvard Medical School · R37 · FY2024 · AI
Coordinate Regulation of Virulence in Vibrio Cholerae
$999,436John J Mekalanos · Harvard Medical School · R37 · FY2025 · AI
Protein Modifications Involved in Cell Signaling
$999,153Aleksandra Nita-Lazar · National Institute Of Allergy And Infectious Diseases · ZIA · FY2023 · AI
Genome instability in cancer: telomeres and DNA repair
$998,533Titia De Lange · Rockefeller University · R35 · FY2025 · CA
The von Hippel-Lindau Tumor Suppressor Gene and Kidney Cancer: Insights into Oxygen Sensing and Treating Cancers Caused by Undruggable Mutations
$997,800William G Kaelin · Dana-Farber Cancer Inst · R35 · FY2025 · CA
The von Hippel-Lindau Tumor Suppressor Gene and Kidney Cancer: Insights into Oxygen Sensing and Treating Cancers Caused by Undruggable Mutations
$997,800William G Kaelin · Dana-Farber Cancer Inst · R35 · FY2023 · CA
Motor proteins and cytoskeletal dynamics in lymphocytes, melanocytes and neurons
$997,625John A Hammer · National Heart, Lung, And Blood Institute · ZIA · FY2018 · HL
Discovery, Regulation and Function of the PI 3-Kinase and AKT Pathway in Cancer
$997,501Alex Toker · Beth Israel Deaconess Medical Center · R35 · FY2024 · CA
Mechanisms of Therapy and Model Development in Viral Hepatitis and Liver Diseases
$997,377T. Jake Liang · National Institute Of Diabetes And Digestive And Kidney Diseases · ZIA · FY2019 · DK
Identifying Metabolic Dependencies of Pancreatic Cancers
$996,661Alec Kimmelman · New York University School Of Medicine · R35 · FY2022 · CA
Differential function and tumor vulnerabilities revealed by RAS membrane trafficking
$996,661Mark Reid Philips · New York University School Of Medicine · R35 · FY2022 · CA
Identifying Metabolic Dependencies of Pancreatic Cancers
$996,661Alec Kimmelman · New York University School Of Medicine · R35 · FY2023 · CA
Decoding And Targeting The LKB1-AMPK Signaling Pathway In Cancer
$996,270Reuben J Shaw · Salk Institute For Biological Studies · R35 · FY2017 · CA
Laboratory of Oral Connective Tissue Biology
$996,009Martha Somerman · National Institute Of Arthritis And Musculoskeletal And Skin Diseases · ZIA · FY2016 · AR
Technology Development for 3D Electron Microscopy
$994,049Sriram Subramaniam · Division Of Basic Sciences - Nci · ZIA · FY2018 · CA
Optimization of an in vivo base editing strategy to treat SOD1-linked ALS
$993,968Thomas Gaj · University Of Illinois At Urbana-Champaign · U01 · FY2025 · NS
High-throughput development and characterization of compact tools for transcriptional and chromatin perturbations
$992,642Lacramioara Bintu · Stanford University · R01 · FY2022 · HG
High-throughput development and characterization of compact tools for transcriptional and chromatin perturbations
$992,590Lacramioara Bintu · Stanford University · R01 · FY2023 · HG
Bay Area Cancer Target Discovery and Development
$992,465Michael T McManus · University Of California, San Francisco · U01 · FY2025 · CA