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17,828 grants matching “crispr”
Core D GET iN
$121,273Irina V Budunova · Northwestern University At Chicago · P30 · FY2019 · AR
Core D GET iN
$121,273Irina V Budunova · Northwestern University At Chicago · P30 · FY2020 · AR
Core D GET iN
$121,272Irina V Budunova · Northwestern University At Chicago · P30 · FY2021 · AR
Functional specialization of compartmentalized beta2-adrenergic signaling in neurons
$121,230Nikoleta Georgieva Tsvetanova · University Of California, San Francisco · K99 · FY2017 · MH
Functional specialization of compartmentalized beta2-adrenergic signaling in neurons
$121,230Nikoleta Georgieva Tsvetanova · University Of California, San Francisco · K99 · FY2016 · MH
Understanding and targeting non-genetic mechanisms of drug resistance
$121,133Jing Lin Xie · Stanford University · K99 · FY2023 · AI
Understanding and targeting non-genetic mechanisms of drug resistance
$121,133Jing Lin Xie · Stanford University · K99 · FY2024 · AI
The Role of Retrotransposon Activity in Mammalian Pre-Implantation Development
$120,960Andrew Joseph Modzelewski · University Of California Berkeley · K99 · FY2018 · HD
The Role of Retrotransposon Activity in Mammalian Pre-Implantation Development
$120,960Andrew Joseph Modzelewski · University Of California Berkeley · K99 · FY2019 · HD
Epigenomic regulation of oxidative stress-producing innate immunity in neuroinflammation
$120,864Andrew S Mendiola · J. David Gladstone Institutes · K99 · FY2022 · NS
Epigenomic regulation of oxidative stress-producing innate immunity in neuroinflammation
$120,864Andrew S Mendiola · J. David Gladstone Institutes · K99 · FY2023 · NS
New Murine Models for Study of Atrophic Fracture Nonunion
$120,725Matthew J. Silva · Washington University · R21 · FY2021 · AR
Therapeutic rescue of Polycystin-1 protein expression by targeting PKD1 upstream open reading frames
$120,600Whitney Elise Besse · Yale University · R03 · FY2024 · DK
USING SINGLE-CELL CRISPR SCREEN TO DECIPHER THE FUNCTION OF CORE TRANSCRIPTION FACTORS IN DRIVING NF1-DEFICIENT MPNST
$120,459The Geneva Foundation · · FY2022 · Defense Health Agency
Control of Mitochondrial Function by SPFH Proteins in Pathogenic Yeast
$120,421Jason Malcolm Rauceo · John Jay College Of Criminal Justice · SC3 · FY2022 · GM
Diagnostic Innovations for Pediatric Tuberculosis in Bolivia
$120,406Richard A Oberhelman · Tulane University Of Louisiana · R01 · FY2025 · AI
EAGER: Prokaryotic Adaptive Immunity and the CRISPR Associated Complex for Antiviral Defense (CASCADE)
$120,235Charles M Lawrence · Montana State University · · FY2012 · BIO
Transgenic and Chimeric Mouse Core
$120,063Stephen Aaron Liebhaber · University Of Pennsylvania · P30 · FY2017 · DK
EARLY PREGNANCY LOSS IS A MAJOR HURDLE THAT THE CATTLE INDUSTRY FACES.INDEED, NEARLY HALFOF BEEF COWS WILL BE NONPREGNANT AT DAY 30 FOLLOWING A SINGLE INSEMINATION. SIMILARLY, EARLY PREGNANCY LOSS IN LACTATING DAIRY COWS CAN BE AS GREAT AS 46%. THIS LEADS TO NEGATIVE ECONOMIC EFFECTS THAT STEM FROM AN INCREASED NUMBER OF NONPREGNANT COWS ACCRUING MAINTENANCE COSTS, FEWER TOTAL POUNDS OF CALF AT WEANING, REDUCED LIFETIME MILK PRODUCTION AND INCREASED CULLING IN DAIRY COWS. SUBSTANTIAL EMBRYONIC LOSSES (13-24%) OCCUR DURING BLASTOCYST HATCHING AND EMBRYO ELONGATION, BUT LITTLE PROGRESS HAS BEEN MADE TO ELUCIDATE FACTOR(S) RESPONSIBLE FOR THESE LOSSES. THIS PROPOSAL WORKS TO INVESTIGATE EMBRYONIC LOSSES OCCURRING AT THIS TIME POINT IN DEVELOPMENT.BASED ON PRELIMINARY DATA FROM OUR LAB INVESTIGATING AN EMBRYOKINE CALLED INTERLEUKIN-6 (IL6), THE PROJECT DIRECTOR DEVELOPED ACENTRAL HYPOTHESIS OF THIS PROPOSAL WHICHIS THATIL6 FUNCTIONS AS A KEY FACILITATOR OF EARLY EMBRYONIC DEVELOPMENT IN CATTLE AND THUS, IT IS ESSENTIAL FOR NORMAL DEVELOPMENT IN VIVO. SHEWILL OBJECTIVELY TEST THIS CENTRAL HYPOTHESIS USING CRISPR-CAS9 GENE EDITING AND RNA SEQUENCING TECHNOLOGY IN ADDITION TO OTHER BIOCHEMICAL AND MOLECULAR TECHNIQUES. COLLECTIVELY, THE EXPERIMENTS CONTAINED WITHIN THIS PROPOSAL WILL PROVIDE NOVEL INSIGHTS INTO INNER CELL MASS DEVELOPMENT AND BLASTOCYST ELONGATION IN CATTLE.
$120,000Virginia Polytechnic Institute & State University · · FY2021 · National Institute of Food and Agriculture
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** NOROVIRUS (NOV) IS AN EXTREMELY PREVALENT FOODBORNE PATHOGEN THAT CAUSES SIGNIFICANT HUMAN AND ECONOMIC LOSSES EVERY YEAR. CURRENT DETECTION METHODS, RELYING ON POLYMERASE CHAIN REACTION (PCR) TECHNOLOGY, ARE COMPLEX AND OFTEN INACCESSIBLE. THIS PROJECT PROPOSES A NOVEL SOLUTION: A CRISPR-CAS14 G-QUADRUPLEX (CRISPR-G4) BIOSENSOR FOR ON-SITE DETECTION OF NOV IN LEAFY GREENS. OUR SYSTEM FIRST AMPLIFIES NOV RNA ISOTHERMALLY USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP). WE WILL THEN UTILIZE A CRISPR-CAS14 SYSTEM FOR EXTREMELY SPECIFIC AND ROBUST TARGET RECOGNITION. THIS SYSTEM PROVIDES A FRONTIER INTO THE NEXT GENERATION OF CRISPRBASED BIOSENSORS, DRAWING ON THE COMPACT SIZE AND BIOSENSING CAPABILITIES OF THE CAS14 ENZYME. THE SYSTEM IS THEN COMBINED WITH G4S TO PRODUCE A SIMPLE COLORIMETRIC READOUT. THIS APPROACH REQUIRES NO SPECIALIZED EQUIPMENT, ENABLING RAPID AND AFFORDABLE ON-SITE TESTING. WE PROPOSE THREE OBJECTIVES, INCLUDING:1) THE DESIGN OF THE CRISPR-CAS14 SYSTEM FOR THE DETECTION OF NOV RNA,2) THE DEVELOPMENT OF THE CRISPR-G4 SYSTEM,3) VALIDATION OF THE EFFICACY IN DETECTING NOV IN LEAFY GREENS.BASED ON OUR EXPERTISE AND PREVIOUS LITERATURE, WE ANTICIPATE THAT THE PROPOSED BIOSENSOR WILL PROVIDE EXTREMELY SENSITIVE AND SPECIFIC RECOGNITION OF NOV IN SPINACH AND LETTUCE AND COULD POTENTIALLY BE EXTENDED FOR SIMILAR SYSTEMS. THE PROJECT ALIGNS SEAMLESSLY WITH THE FOOD SAFETY NUTRITION, AND HEALTH PRIORITY AREA DUE TO THE POTENTIAL OF OUR PROPOSED SENSOR TO COMBAT FOODBORNE ILLNESSES. WE ANTICIPATE THAT OUR SENSOR WILL SUBSTANTIALLY ADDRESS THE URGENT NEED FOR ACCESSIBLE NOV TESTING, POTENTIALLY REDUCING FOODBORNE ILLNESSES AND ECONOMIC LOSS.
$120,000Virginia Polytechnic Institute & State University · · FY2024 · National Institute of Food and Agriculture
Gene regulation for stem cell differentiation
$119,962Jamy C. Peng · St. Jude Children'S Research Hospital · R01 · FY2021 · GM
Center for Medical Genomics
$119,951Yunlong Liu · Indiana University Indianapolis · P30 · FY2021 · CA
Center for Medical Genomics
$119,951Yunlong Liu · Indiana University Indianapolis · P30 · FY2022 · CA
Center for Medical Genomics
$119,951Yunlong Liu · Indiana University Indianapolis · P30 · FY2023 · CA
Center for Medical Genomics
$119,951Yunlong Liu · Indiana University Indianapolis · P30 · FY2020 · CA