Sort
17,828 grants matching “crispr”
Project 4: Mechanisms and Impacts of PCB Resistance in Fish
$180,512Mark E Hahn · Boston University Medical Campus · P42 · FY2017 · ES
Building a Comprehensive Discovery System for Understanding Bacterial Multidrug Resistance and Pathogenesis Using Tn7-like Elements
$180,496Joseph E Peters · Cornell University · R21 · FY2020 · AI
RII Track-4: The Integration of Plasmonic Nanoantenna and Super-hydrophobic Surface for Ultrasensitive Fluorescence CRISPR Biosensing
$180,493Shengjie Zhai · University Of Nevada Las Vegas · · FY2022 · O/D
Core D: CRISPRi/a Core
$180,271Martin Kampmann · Weill Medical Coll Of Cornell Univ · U54 · FY2020 · NS
Synthetic organogenesis: new paradigms in reconstituting human organ development in vitro
$180,220Mijo Simunovic · Columbia Univ New York Morningside · DP2 · FY2022 · HD
Molecular and genetic decoding of neuron-glial interactions
$180,201Lu Sun · Ut Southwestern Medical Center · DP2 · FY2023 · MH
Transgenic & Knock-out Mouse
$180,065Pamela L Mellon · University Of California, San Diego · P30 · FY2020 · DK
Functional Genomics
$180,051Nevan J Krogan · University Of California, San Francisco · U54 · FY2020 · CA
Functional Genomics Shared Resource
$180,039Dan Theodorescu · University Of Colorado Denver · P30 · FY2018 · CA
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** BOVINE MASTITIS IS A CONSTANT PROBLEM IN THE DAIRY INDUSTRY. MILK QUALITY IS AFFECTED, PRODUCTION IS REDUCED, AND ANTIBIOTICS ARE NEEDED FOR TREATMENT WHEN DAIRY COWS ARE AFFECTED BY MASTITIS. ONE OF THE MOST PREVALENT MASTITIS CAUSING BACTERIA IS STAPHYLOCOCCUS AUREUS (S. AUREUS). A POSSIBLE ALTERNATIVE TO ANTIBIOTICS IS THE USE OF BACTERIOPHAGES (PHAGES), WHICH ARE VIRUSES THAT ONLY INFECT BACTERIA. A MAJOR LIMITATION FOR PHAGES TO BE USED AS A TREATMENT FOR BOVINE MASTITIS IS WHEY PROTEIN'S ANTIVIRAL ACTIVITY. THE OVERALL GOAL IS TO INCREASE BACTERIOPHAGE (PHAGE) INFECTIVITY, THUS INCREASING ITS THERAPEUTIC POTENTIAL, OF MASTITIS CAUSING S. AUREUS BY REDUCING ITS INTERACTION WITH MILK ANTIVIRAL MECHANISMS. WE WILL DEVELOP A CELL-FREE TRANSCRIPTION-TRANSLATION (TXTL) SYSTEM DERIVED FROM STAPHYLOCOCCUS AUREUS. THE TXTL SYSTEM DOES NOT PRODUCE ENDOTOXINS OR EXOTOXINS DURING PHAGE PRODUCTION, WHICH IS A MAJOR PROBLEM IN THE CURRENT IN VIVO METHOD OF PHAGE PRODUCTION. IT WILL BE USED TO PRODUCE GREEN FLORESCENT PROTEIN AND WHOLE PHAGES FROM CIRCULARIZED PLASMID DNA. MILK WHEY PROTEIN ANTIVIRAL ACTIVITY IS A HINDRANCE TO PHAGE TREATMENT FOR MASTITIS. WE WILL BE USING THE CRISPR-CAS9 ENGINEERING METHOD TO GENETICALLY MODIFY THE SURFACE OF PHAGE CAPSIDS TO OBTAIN THE POLYMER POLYETHYLENE GLYCOL (PEG) TO CONCEAL THE PHAGE FROM THE ANTIVIRAL ACTIVITY IN RAW MILK LONG ENOUGH TO ELIMINATE S. AUREUS CONTAMINATION.THE RESULTS OF THIS PROJECT WILL BE INSTRUMENTAL IN ADVANCING THE USE OF PHAGES AS AN ANTIMICROBIAL THERAPY IN ANIMAL HUSBANDRY. THE TXTL SYSTEM WILL INCREASE THE SPEED IN WHICH WE CAN PRODUCE AND EVALUATE VIRULENT PHAGES FOR THE USE OF TREATMENT. THE ENGINEERED PHAGES FOR THE TREATMENT OF S. AUREUS IN RAW MILK SAMPLES WILL BRING US ONE STEP CLOSER TO REDUCING THE USE OF ANTIBIOTICS IN THE USE OF TREATING INFECTIONS IN THE DAIRY INDUSTRY AND REDUCE THE SPREAD OF ANTIMICROBIAL RESISTANT BACTERIA.
$180,000Cornell University · · FY2023 · National Institute of Food and Agriculture
Hepatocyte-targeted somatic-cell genetic complementation in mice
$180,000Edward E Schmidt · Montana State University - Bozeman · R21 · FY2020 · OD
Exploiting iPLA2β-modified macrophages as immunotherapy for T1D
$179,955Sasanka Ramanadham · University Of Alabama At Birmingham · R21 · FY2023 · AI
Targeting SETBP1 in Myeloproliferative Disorders
$179,928Elliot Stieglitz · University Of California, San Francisco · K08 · FY2020 · HL
Targeting SETBP1 in Myeloproliferative Disorders
$179,928Elliot Stieglitz · University Of California, San Francisco · K08 · FY2021 · HL
Targeting SETBP1 in Myeloproliferative Disorders
$179,928Elliot Stieglitz · University Of California, San Francisco · K08 · FY2019 · HL
Targeting SETBP1 in Myeloproliferative Disorders
$179,928Elliot Stieglitz · University Of California, San Francisco · K08 · FY2017 · HL
Project 5: A Novel Mechanism of Ortho-PCB-induced Toxicity: Targeting Nuclear Receptors in Brain of Fish
$179,918John J Stegeman · Boston University Medical Campus · P42 · FY2017 · ES
Identifying Novel Radiation Sensitizers in Cervical Cancer
$179,870Lauren Elizabeth Colbert · University Of Tx Md Anderson Can Ctr · R21 · FY2024 · CA
EAGER: Systematic Approach to Improve Probability of Metabolic Engineering Success
$179,779Mattheos Koffas · Rensselaer Polytechnic Institute · · FY2014 · BIO
Regulation and function of developmentally programmed 3 CpG island methylation
$179,750Lanlan Shen · Baylor College Of Medicine · R21 · FY2021 · HD
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** SILK HAS BEEN AN IMPORTANT MATERIAL THROUGHOUT HISTORY, USED IN TEXTILES, COSMETICS, AND MEDICAL APPLICATIONS. CURRENTLY, THE GLOBAL SILK MARKET IS EXPANDING AS GROUPS AIM TO FIND A MOREENVIRONMENTALLY FRIENDLY ALTERNATIVETO SYNTHETIC MATERIALS.HOWEVER, THE UNITED STATES HAS FACED CHALLENGES IN DEVELOPING A SUCCESSFUL SILK MANUFACTURING INDUSTRY, MISSING OUT ON THE EXPANDING GLOBAL MARKET. HIGHLIGHTINGTHE NEED FOR INNOVATIVE APPROACHES TO SILK PRODUCTION.TRADITIONALLY, SILK MANUFACTURING RELIES ON THE DOMESTICATED SILKWORM, BOMBYX MORI. CURRENT SILK FARMING PRACTICES ARE LABOR-INTENSIVE AND NOT WELL-SUITED FOR HIGH LABOR COST AREAS LIKE THE USA. ADDITIONALLY, OUTDOOR SILK FARMING FACES CHALLENGES SUCH AS INCONSISTENT QUALITY AND SUPPLY CHAIN ISSUES, WHICH CAN BE EXASERBATED BY CLIMATE CHANGE. FURTHERMORE, RECENT ADVANCEMENTS IN GENETIC ENGINEERING, LIKE CRISPR TECHNOLOGIES, HAVE OPENED NEW POSSIBILITIES FOR USING SILKWORMS TO PRODUCE VALUABLE PROTEINS. HOWEVER, PRODUCING LARGE PROTEINS WITH COMMON METHODS, SUCH AS USING E. COLI BACTERIA, IS DIFFICULT. SILK GLANDS, ON THE OTHER HAND, ARE WELL-SUITED FOR THIS TASK.IN MY PROJECT, I AIM TO LEVERAGE THE INDIAN MEAL MOTH, PLODIA INTERPUNCTELLA (PI), AS AN ALTERNATIVE SILK PRODUCER FOR BOTH NUTURAL AND MODIFIED SILK. PI CAN BE EASILY RAISED INDOORS WITH CONTROLLED CONDITIONS AND STANDARDIZED FOOD SOURCES. IT IS ALSO A GOOD MODEL FOR GENETIC ENGINEERING, SUPPORTED BY EXTENSIVE GENETIC DATA. BY UNDERSTANDING HOW TO ENHANCE SILK PRODUCTION AND DEVELOPING METHODS FOR COLLECTING SILK AND EXPRESSING MODIFIED SILK PROTEINS, PI COULD HELP THE USA ENTER THE GLOBAL SILK MARKET. THIS APPROACH WOULD OFFER BETTER STANDARDIZATION AND INDUSTRIAL MANUFACTURING PROCESSES WHILE MINIMIZING THE RELEASE OF GENETICALLY MODIFIED ORGANISMS INTO THE ENVIRONMENT.
$179,742University Of Florida · · FY2024 · National Institute of Food and Agriculture
A biotherapeutic CRISPR-delivery platform to eradicate Clostridium difficile
$179,732Jan-Peter Van Pijkeren · University Of Wisconsin-Madison · R21 · FY2016 · AI
Molecular tools for targeting RNA
$179,720Patrick Hsu · University Of California Berkeley · R01 · FY2022 · GM
Motoki Project
$179,494Motoki Takaku · University Of North Dakota · P20 · FY2022 · GM
Targeting RHNO1 in Ovarian Cancer
$179,403Adam R Karpf · University Of Nebraska Medical Center · R21 · FY2023 · CA