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17,054 grants matching “genome editing”
Synthetic organogenesis: new paradigms in reconstituting human organ development in vitro
$180,220Mijo Simunovic · Columbia Univ New York Morningside · DP2 · FY2022 · HD
NRSA Training Core
$180,193Eric N Olson · Ut Southwestern Medical Center · U54 · FY2017 · HD
NRSA Training Core
$180,193Eric N Olson · Ut Southwestern Medical Center · U54 · FY2018 · HD
Administrative Core
$180,143Johann Eli Gudjonsson · University Of Michigan At Ann Arbor · P30 · FY2025 · AR
NRSA Training Core
$180,055Eric N Olson · Ut Southwestern Medical Center · U54 · FY2016 · HD
Functional Genomics
$180,051Nevan J Krogan · University Of California, San Francisco · U54 · FY2020 · CA
Functional Genomics Shared Resource
$180,039Dan Theodorescu · University Of Colorado Denver · P30 · FY2018 · CA
Gene Vector Virus Core
$180,022Javier Alcudia · Stanford University · P30 · FY2016 · NS
Role of Dopamine Neuron-Specific Gene Enhancers in Cocaine Relapse
$180,009Luis Miguel Tuesta · University Of Miami School Of Medicine · K01 · FY2020 · DA
Hepatocyte-targeted somatic-cell genetic complementation in mice
$180,000Edward E Schmidt · Montana State University - Bozeman · R21 · FY2020 · OD
SOYBEAN IS ONE OF THE MOST IMPORTANT CROP PLANTS WORLDWIDE AS IT IS A PRIMARY SOURCE OF PROTEIN AND VEGETABLE OIL IN FEED USED TO PRODUCE BEEF, HOGS, AND CHICKENS, AS WELL AS BEING CONSUMED DIRECTLY BY HUMANS. HOWEVER, THERE ARE A VARIETY OF DISEASES AND PESTS THAT JEOPARDIZE OVERALL SOYBEAN HEALTH AND ULTIMATELY DECREASE SOYBEAN YIELDS. ONE OF THE MOST DAMAGING PATHOGENS OF SOYBEAN IN NORTH AMERICA IS THE SOYBEAN CYST NEMATODE (SCN), WHICH INFECTS THE PLANT'S ROOTS. CURRENT METHODS TO PREVENT SOYBEAN CYST NEMATODE INFECTION ONLY PROVIDE LIMITED PROTECTION AND THE OVERUSE OF THESE STRATEGIES HAVE RESULTED IN DECREASED EFFICIENCY AS THE NEMATODES HAVE DEVELOPED WAYS TO OVERCOME THEM. DUE TO THIS, NEW METHODS TO PREVENT SOYBEAN CYST NEMATODE INFECTION ARE IN HIGH DEMAND. WITHOUT NEW MANAGEMENT STRATEGIES, A WIDE ARRAY OF PRODUCTS THAT COME FROM SOYBEAN ARE AT RISK OF BECOMING SCARCE OR UNAVAILABLE. TO DEVELOP NEW METHODS FOR COMBATTING SCN, IT IS IMPORTANT TO UNDERSTAND HOW SCN DEVELOPS AND MAINTAINS INFECTION IN SOYBEAN ROOTS. IT IS WIDELY KNOWN IN THE RESEARCH COMMUNITY THAT SCN DELIVERS SALIVARY SECRETIONS INTO PLANT CELLS. IN THESE SALIVARY SECRETIONS ARE PROTEINS KNOWN AS EFFECTORS. EFFECTOR PROTEINS ARE KNOWN TO HIJACK AND REPROGRAM HOST PLANT CELLS FOR THE PATHOGEN'S BENEFIT. AS THE EFFECTOR PROTEINS SECRETED BY SCN ARE KNOWN TO BE ONE OF THE MOST IMPORTANT ASPECTS OF INFECTION, I HAVE SELECTED 11 UNCHARACTERIZED SCN EFFECTOR PROTEINS WITH THE OVERALL GOAL OF UNDERSTANDING THE SPECIFIC FUNCTIONS OF THESE PROTEINS AS WELL AS IDENTIFYING THE PLANT PATHWAYS THEY DISRUPT. I WILL FIRST BE INVESTIGATING IF THE SCN EFFECTOR PROTEINS INTERFERE WITH PLANT IMMUNE RESPONSES. IF A PROTEIN DOES INTERFERE WITH PLANT IMMUNITY, I WILL THEN IDENTIFY THE SPECIFIC PLANT PROTEIN THAT IT TARGETS. IDENTIFYING THE PLANT PROTEIN(S) TARGETED BY THE EFFECTOR PROTEIN WILL BE THE FIRST STEP TO UNDERSTANDING HOW THE PLANT IS IMPACTED BY THE NEMATODE. AFTER IDENTIFYING THE SCN EFFECTOR PROTEIN TARGETS, I WILL REMOVE THE GENE THAT PRODUCES THE PROTEIN FROM THE PLANT GENOME. THIS WILL BE ACCOMPLISHED USING GENOME EDITING IN SOYBEAN ROOTS. THE ROOTS THAT LACK THE EFFECTOR PROTEIN TARGET WILL BE INFECTED WITH SCN IN THE LABORATORY TO DETERMINE THE ROLE THAT THE PLANT PROTEIN PLAYS IN THE INFECTION PROCESS. IT IS POSSIBLE THAT REMOVING A GENE FROM THE PLANT GENOME WILL DECREASE THE NEMATODE'S VIRULENCE WITHOUT HARMING THE PLANT. RESULTS FROM THIS RESEARCH WILL ESTABLISH AN IMPORTANT FOUNDATION FOR EXPLORING THE PLANT PATHWAYS AND PROTEINS THAT SHOULD BE OF INTEREST WHEN DEVELOPING NEW SOYBEAN VARIETIES THAT ARE RESISTANT TO SCN INFECTION. ADDITIONALLY, THE FINDINGS OF THIS RESEARCH HAVE GREAT POTENTIAL TO PROVIDE INSIGHT INTO HOW OTHER PLANT-PARASITIC NEMATODES ESTABLISH INFECTION. THROUGHOUT THIS PROJECT, I WILL BE MENTORED BY DR. ROGER INNES, A LEADER IN PLANT-PATHOGEN INTERACTIONS, AND DR. THOMAS BAUM, AND EXPERT IN NEMATOLOGY, WHICH WILL ALLOW FOR A DEEP UNDERSTANDING OF THE RELATIONSHIP BETWEEN THE EFFECTOR PROTEINS SECRETED BY SCN AND THE HOST PLANT.TOGETHER, THESE EXPERIMENTS WILL PROVIDE A FOUNDATION OF KNOWLEDGE THAT IS ESSENTIAL FOR DEVELOPING NEW VARIETIES OF SOYBEAN THAT ARE RESISTANT TO SCN, WHICH WILL IMPROVE FOOD SECURITY IN THE USA AND THE WORLD.
$180,000Trustees Of Indiana University · · FY2022 · National Institute of Food and Agriculture
Molecular Biology Core
$179,955Juanita L Merchant · University Of Michigan At Ann Arbor · P30 · FY2020 · DK
Collaborative Research: Deep-sequencing analysis of edited metabolic pathways to uncover, model, and overcome the epistatic constraints upon optimization
$179,867Jeremy Draghi · Virginia Polytechnic Institute And State University · · FY2019 · BIO
Molecular tools for targeting RNA
$179,720Patrick Hsu · University Of California Berkeley · R01 · FY2022 · GM
Role of Dopamine Neuron-Specific Gene Enhancers in Cocaine Relapse
$179,655Luis Miguel Tuesta · University Of Miami School Of Medicine · K01 · FY2021 · DA
Role of Dopamine Neuron-Specific Gene Enhancers in Cocaine Relapse
$179,655Luis Miguel Tuesta · University Of Miami School Of Medicine · K01 · FY2022 · DA
Rat Models for Sex Steroid Action
$179,501Michael J Soares · University Of Kansas Medical Center · R21 · FY2013 · OD
Experimental and preclinical modeling of NUP98-rearranged acute leukemia
$179,500Charles G. Mullighan · St. Jude Children'S Research Hospital · U54 · FY2020 · CA
Motoki Project
$179,494Motoki Takaku · University Of North Dakota · P20 · FY2022 · GM
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** AS CROP YIELDS BECOME MORE VARIABLE IN RESPONSE TO GLOBAL CLIMATE CHANGE, IT IS IMPERATIVE TO PRIORITIZE THE PROTECTION OF COMMODITIES AFTER HARVEST FROM STORED PRODUCT INSECT DAMAGE TO PRESERVE FOOD SECURITY. POST-HARVEST FOOD SECURITY RELIES ON INTEGRATED PEST MANAGEMENT TECHNIQUES TO REDUCE PEST POPULATION LEVELS WHICH INCLUDES SANITATION, THE APPLICATION OF INSECTICIDES OR FUMIGANTS TO REDUCING PEST POPULATIONS, AND BEHAVIORAL BASED MANAGEMENT APPLICATIONS. BEHAVIORALLY BASED MANAGEMENT PRACTICES EXPLOIT BEHAVIOR TO DETECT AND MANIPULATE PEST POPULATIONS USING VOLATILE ORGANIC COMPOUNDS, SUCH AS PHEROMONES AND KAIROMONES PRODUCED BY CONSPECIFICS AND FOOD SOURCES, RESPECTIVELY. ONE OF THE MOST DAMAGING COSMOPOLITAN STORED PRODUCT PESTS PLODIA INTERPUNCTELLA (INDIANMEAL MOTH; IMM) STRONGLY RELIES ON PHEROMONES AND KAIROMONES TO LOCATE MATES AND HABITATS FOR FEEDING AND OVIPOSITION. IN SOME ENVIRONMENTS, FLOOD THE ENVIRONMENT WITH PHEROMONE TO MAKE IT DIFFICULT TO FIND MATES IS COMMONLY USED TO REDUCE PEST POPULATION. THIS TACTIC IS KNOWN AS MATING DISRUPTION AND ALTHOUGH IT CAN BE VERY EFFECTIVE IN SOME ENVIRONMENTS, ITS EFFICACY CAN BE VARIABLE DUE TO GENETIC DIFFERENCES THAT ALLOW FOR SOME MALES TO OVERCOME THE INCREASED PHEROMONE AMONG MOTH POPULATIONS. IMM USES OLFACTORY PROTEIN RECEPTORS EXPRESSED IN ANTENNAE TO PERCEIVE THESE COMPOUNDS; HOWEVER, LITTLE WORK HAS BEEN DONE TO IDENTIFY AND CHARACTERIZE THE FUNCTION OF THE RECEPTOR GENES THAT INFLUENCE IMM ATTRACTION TO VOLATILES.TO UNDERSTAND HOW IMM PERCEIVES AND RESPONDS TO PHEROMONES AND FOOD VOLATILES, WE WILL FIRST CURATE GENES WITH PREDICTED INVOLVEMENT IN OLFACTION FROM A GENOME ASSEMBLY GENERATED BY THE USDA-ARS AG100PEST INITIATIVE. GENES WILL BE PREDICTED USING A COMPUTATIONAL PIPELINE, BUT THESE PREDICTIONS WILL BE REFINED AND EXPANDED USING GENE MODELS FROM OTHER CLOSELY RELATED MOTH SPECIES AS GUIDES. IN ADDITION, GENE EXPRESSION DATASETS FROM IMM ANTENNAE AND OTHER CHEMOSENSORY ORGANS WILL BE USED TO FIND NEW GENE MODELS IN THIS SPECIES THAT MAY NOT BE FOUND IN OTHER MOTH SPECIES. GENES WHOSE EXPRESSION LEVELS CORRELATE WITH RESPONSES TO PHEROMONE AND FOOD OILS WILL BE IDENTIFIED USING HIGH THROUGHPUT GENE EXPRESSION EXPERIMENTS (RNA-SEQ). IN BRIEF, THE RESPONSIVENESS OF MATED AND UNMATED MALES AND FEMALES TO PHEROMONES WILL BE MONITORED OVER THEIR LIFE SPAN TO IDENTIFY PERIODS OF RESPONSIVENESS AND LOW RESPONSIVENESS. GENE EXPRESSION OF INDIVIDUALS FROM HIGHLY RESPONSIVE TREATMENTS WILL BE COMPARED TO LOWLY RESPONSIVE TREATMENTS TO IDENTIFY GENES THAT ARE IMPORTANT FOR THE PERCEPTION OF PHEROMONES. IN ADDITION, A SIMILAR EXPERIMENT WILL BE PERFORMED USING VOLATILES COLLECTED FROM FLOUR, WHICH IS A HIGHLY ATTRACTIVE FOOD SOURCE TO IDENTIFY GENES THAT ARE IMPORTANT FOR THE PERCEPTION OF PHEROMONES. THE FUNCTION OF AT LEAST TWO GENES WILL BE VALIDATED USING GENE EDITING APPROACHES TO DETERMINE WHETHER DISRUPTING GENE FUNCTION WILL DISRUPT IMM'S ABILITY TO PERCEIVE AND RESPOND,TO PHEROMONES AND FOOD VOLATILES.INTERFERING WITH AN INSECT'S ABILITY TO FIND FOOD OR ITS MATES MAY BE EXPLOITED TO IMPROVE TRAPS USED FOR MONITORING INSECT ACTIVITY OR TO ALTER INSECT MOVEMENT AND BEHAVIOR THROUGH NEW STRATEGIES OF PREVENTION BY MANIPULATING THE INSECT'S MOVEMENT AWAY FROM STORAGE STRUCTURES GRAIN BINS, ELEVATORS, FOOD PROCESSING FACILITIES, AND WAREHOUSES AND TOWARD A DESIRED AREA LIKE A TRAP. FURTHERMORE, THIS RESEARCH CAN ALSO HELP DETERMINE WHETHER GENETIC DIFFERENCES AMONG POPULATIONS ARE RESPONSIBLE FOR THE VARIABLE EFFICACY OF BEHAVIORALLY BASED MANAGEMENT. OVERALL, THIS STUDY HIGHLIGHTS THE IMPORTANCE OF INTEGRATED PEST MANAGEMENT TECHNIQUES FOR POST-HARVEST FOOD SECURITY AND SHEDS LIGHT ON THE GENETIC FACTORS THAT INFLUENCE INSECT BEHAVIOR.
$179,395Kansas State University · · FY2023 · National Institute of Food and Agriculture
The role of macroH2A-ZMYM3 in genome stability and tumorigenesis
$179,388Justin Wai Chung Leung · Univ Of Arkansas For Med Scis · K22 · FY2020 · CA
The role of macroH2A-ZMYM3 in genome stability and tumorigenesis
$179,388Justin Wai Chung Leung · Univ Of Arkansas For Med Scis · K22 · FY2019 · CA
Therapeutic potential of targeting DNA repair deficiency in TSC
$179,252Jeffrey Paul Mackeigan · Michigan State University · R21 · FY2022 · CA
Higher Order Chromatin and Genetic Risk for Alzheimer's Disease
$179,162Panagiotis Roussos · Icahn School Of Medicine At Mount Sinai · R01 · FY2018 · AG
Genome Engineering and iPSC Center
$179,132Timothy J Eberlein · Washington University · P30 · FY2025 · CA