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17,828 grants matching “crispr”
A New IFN-γ Activated Cell Death Pathway in Salmonella-infected Non-Phagocytic Cells
$274,500Siddharth Balachandran · Research Inst Of Fox Chase Can Ctr · R21 · FY2020 · AI
Experimental identification of functional GWAS variants linked to COVID-19 severity in immune cells
$274,500Benjamin Joachim Schmiedel · La Jolla Institute For Immunology · R21 · FY2024 · AI
Enhancing E. coli Nissle colonization with CRISPR-Cas immunity
$274,401Matthew Harrison Foley · Ancilia, Inc. · R41 · FY2024 · DK
Project 3 - Novel Therapies for Spliceosomal-Mutant MDS.
$274,015Matthew J Walter · Washington University · P50 · FY2021 · CA
Steroid signaling in the choroid plexus of the aging brain
$273,807Polina V Lishko · University Of California Berkeley · R03 · FY2021 · AG
Transgenic Mouse, ICSI & IVF Core
$273,756Stefan Moisyadi · University Of Hawaii At Manoa · P30 · FY2019 · GM
Structure, Function and Application of CRISPR RNA-guided Immunity in Bacteria
$273,600Blake A Wiedenheft · Montana State University - Bozeman · R01 · FY2018 · GM
Structure, Function and Application of CRISPR RNA-guided Immunity in Bacteria
$273,600Blake A Wiedenheft · Montana State University - Bozeman · R01 · FY2015 · GM
Structure, Function and Application of CRISPR RNA-guided Immunity in Bacteria
$273,600Blake A Wiedenheft · Montana State University - Bozeman · R01 · FY2014 · GM
Structure, Function and Application of CRISPR RNA-guided Immunity in Bacteria
$273,600Blake A Wiedenheft · Montana State University - Bozeman · R01 · FY2016 · GM
Structure, Function and Application of CRISPR RNA-guided Immunity in Bacteria
$273,600Blake A Wiedenheft · Montana State University - Bozeman · R01 · FY2017 · GM
Structure, Function and Application of CRISPR RNA-guided Immunity in Bacteria
$273,600Blake A Wiedenheft · Montana State University - Bozeman · R01 · FY2019 · GM
Sequencing Technologies & Analysis Shared Resource
$273,281William Richard McCombie · Cold Spring Harbor Laboratory · P30 · FY2025 · CA
Sequencing Technologies & Analysis Shared Resource
$273,281William Richard McCombie · Cold Spring Harbor Laboratory · P30 · FY2022 · CA
Sequencing Technologies & Analysis Shared Resource
$273,281William Richard McCombie · Cold Spring Harbor Laboratory · P30 · FY2021 · CA
Sequencing Technologies & Analysis Shared Resource
$273,281William Richard McCombie · Cold Spring Harbor Laboratory · P30 · FY2023 · CA
Molecular Screening Shared Resource (MSSR)
$273,137Robert Damoiseaux · University Of California Los Angeles · P30 · FY2020 · CA
Mechanisms of CRISPR Interference
$273,121Erik J Sontheimer · Northwestern University · R01 · FY2013 · GM
Cell intrinsic and extrinsic effects of zinc metabolism in therapy resistant melanoma
$273,065Katherine Marie Aird · Wistar Institute · R01 · FY2025 · CA
Determination of chromatin protein dynamics in CD8 T cells
$273,000Yongqiang Feng · St. Jude Children'S Research Hospital · R21 · FY2023 · AI
Novel transgenesis and expression technology for nematodes
$272,750Michael L Nonet · Washington University · R01 · FY2022 · GM
Early in vivo expressed antigens and their role in virulence, immune response, and vaccines for coccidioidomycosis
$272,719Bridget Marie Barker · Northern Arizona University · U19 · FY2025 · AI
THE OBJECTIVE OF THIS PROPOSAL IS TO UNDERSTAND THE MECHANISMS USED BY MYCOBACTERIA TO ADAPT TO LIFE UNDER LSMMG AND TO DETERMINE WHETHER THEIR ABILITY TO FORM BIOFILM IS ALTERED UNDER LSMMG. M. MARINUM WILL BE THE MODEL ORGANISM. IT IS A GOOD MODEL FOR THE MORE POTENT HUMAN PATHOGENIC MYCOBACTERIA AS IT IS A BSL2 ORGANISM. M. MARINUM WILL BE GROWN IN HIGH ASPECT RATIO VESSELS USING THE ROTATING CELL CULTURE SYSTEM TO SIMULATE MICROGRAVITY. PREVIOUSLY WE WERE FUNDED BY NASA FOR 1 YEAR TO EXAMINE HOW M. MARINUM ALTERED GROWTH AND GENE EXPRESSION UNDER LSMMG. OUR PREVIOUS TRANSCRIPTOME ANALYSIS SUGGESTS THAT THE REL AND SIGMA FACTOR H (SIGH) STRESS RESPONSE PATHWAYS ARE ACTIVATED WHEN M. MARINUM ARE SUBJECTED TO LSMMG. WE PLAN TO EXTEND THESE STUDIES WITH THIS PROPOSAL. AIM 1 WILL DECREASE TRANSCRIPTION FROM THE REL GENE AND DETERMINE HOW THE LOSS OF THIS PATHWAY ALTERS M. MARINUM SURVIVAL AND ANTIBIOTIC RESISTANCE UNDER LSMMG. WE WILL USE CRISPR INTERFERENCE (CRISPRI) TO DISRUPT REL TRANSCRIPTION AND RNASEQ TO EXAMINE THE TRANSCRIPTOME. THIS WILL CONFIRM OUR HYPOTHESIS THAT THE REL STRINGENT RESPONSE IS NECESSARY FOR THE SURVIVAL OF LSMMG-GROWN M. MARINUM. WE ARE CURRENTLY STUDYING LSMMG AND THE SIGH TRANSCRIPTOME USING A SMALL GRANT FROM THE LASPACE CONSORTIUM. AIM 2 WILL EXAMINE BIOFILM FORMATION UNDER LSMMG USING MICROCARRIERS FOR BACTERIA ATTACHMENT IN THE HARVS. WE WILL ALSO EXAMINE THE TRANSCRIPTOME RESPONSE OF THE BACTERIA IN BIOFILMS. WE WILL USE WILD-TYPE AND CRISPRI-MODIFIED M. MARINUM WITH DECREASED SIGH OR REL TRANSCRIPTS IN AIM 2. WE HYPOTHESIZE THAT BIOFILM FORMATION WILL BE ENHANCED UNDER LSMMG AND THAT THE REL AS WELL AS SIGH STRESS RESPONSE PATHWAYS WILL BE REQUIRED FOR BIOFILM FORMATION. THIS WILL EXTEND OUR UNDERSTANDING OF MYCOBACTERIA SUBJECTED TO SPACE TRAVEL AND POTENTIALLY REVEAL MECHANISMS TO TARGET MYCOBACTERIUM INFECTIONS IN SPACE AND ON EARTH. THE PROPOSED WORK IS THEREFORE CONSISTENT WITH THE OBJECTIVES OF THE NASA SPACE BIOLOGY PROGRAM.
$272,585Louisiana State University · · FY2020 · National Aeronautics and Space Administration
Core 2: In vivo cancer modeling and screening core facility
$272,379Sidi Chen · Yale University · U54 · FY2019 · CA
Delineation of CRISPR-Cas invader defense pathways in Streptococcus thermophilus
$272,275Michael P Terns · University Of Georgia · R01 · FY2013 · GM