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17,828 grants matching “crispr”
In vivo Screening Methods Targeting Levels of Tau Protein within Mouse Brains
$280,875Jiyoen Kim · Baylor College Of Medicine · R21 · FY2025 · AG
Project 4. Targeting genomic instability and evolution in myeloma
$280,769Nikhil C. Munshi · Dana-Farber Cancer Inst · P01 · FY2019 · CA
Project 4. Targeting genomic instability and evolution in myeloma
$280,767Nikhil C. Munshi · Dana-Farber Cancer Inst · P01 · FY2021 · CA
Project 4. Targeting genomic instability and evolution in myeloma
$280,767Nikhil C. Munshi · Dana-Farber Cancer Inst · P01 · FY2020 · CA
Mechanisms of proinflammatory glial activation in retinal degeneration
$280,704Marcello Michael Distasio · Yale University · K08 · FY2025 · EY
Mechanisms of proinflammatory glial activation in retinal degeneration
$280,704Marcello Michael Distasio · Yale University · K08 · FY2024 · EY
Mechanisms and Functional Consequences of Selective miRNA transfer via extracellular vesicles
$280,644James G. Patton · Vanderbilt University · P01 · FY2022 · CA
Project 3. Oncogenomics to identify and validate novel targeted therapies in myeloma
$280,606Kenneth C Anderson · Dana-Farber Cancer Inst · P01 · FY2019 · CA
Project 3. Oncogenomics to identify and validate novel targeted therapies in myeloma
$280,604Kenneth C Anderson · Dana-Farber Cancer Inst · P01 · FY2021 · CA
Project 3. Oncogenomics to identify and validate novel targeted therapies in myeloma
$280,604Kenneth C Anderson · Dana-Farber Cancer Inst · P01 · FY2020 · CA
Project 3. Oncogenomics to identify and validate novel targeted therapies in myeloma
$280,604Kenneth C Anderson · Dana-Farber Cancer Inst · P01 · FY2018 · CA
Acoustic microvortices instrumentation for dosage controlled, high efficiency cell engineering
$280,482Abraham P Lee · University Of California-Irvine · R01 · FY2022 · GM
Virus, Vector and Cell Culture Core
$280,416Erle S Robertson · University Of Pennsylvania · P01 · FY2025 · CA
Virus, Vector and Cell Culture Core
$280,416Erle S Robertson · University Of Pennsylvania · P01 · FY2024 · CA
Biological Core
$280,415Carolyn Ann Worby · University Of Kentucky · P01 · FY2016 · NS
Project 1:Targeting HIF2 and VHL Synthetic Lethal Interactions in Kidney Cancer
$280,057William G Kaelin · Beth Israel Deaconess Medical Center · P50 · FY2024 · CA
THE DEVELOPMENT OF CRISPR-CAS9 AND RELATED TECHNOLOGIES FOR EDITING MAMMALIAN CELLS AND EMBRYOS HAS RESULTED IN UNPRECEDENTED OPPORTUNITIES FOR IMPROVING LIVESTOCK GENETICS. TO TAKE FULL ADVANTAGE OF GENE EDITING TECHNOLOGIES APPLIED TO ANIMALS SUCH AS INTROGRESSION OF ALLELES FOR IMPROVED GENETIC ADVANCE MULTIPLE EDITS ON THE SAME INDIVIDUAL ARE OFTEN REQUIRED WHICH CURRENTLY CONSTITUTES A TECHNOLOGICAL BARRIER FOR FOOD ANIMAL SPECIES. WE HAVE RECENTLY BEEN ABLE TO ISOLATE AND MAINTAIN STABLE BOVINE EMBRYONIC STEM CELLS (BESC) FULFILLING AN UNMET NEED FOR LIVESTOCK SPECIES THAT HAD BEEN PURSUED FOR THE LAST 30 YEARS. WE HAVE FURTHER OPTIMIZED THE CULTURE CONDITIONS FOR BOVINE ESCS AND EXTENDED THE TECHNOLOGY TO SHEEP AND GOAT SPECIES. ESCS REPRESENT AN IDEAL PLATFORM FOR INTRODUCING MULTIPLE GENETIC MODIFICATIONS AS THEY CAN BE CULTURED INDEFINITELY MAINTAIN GENOMIC STABILITY AND ARE AMENABLE TO SCNT/CLONING FOR PRODUCING ESC-DERIVED OFFSPRING. THE GOAL OF THE PROPOSED PROJECT IS TO DEVELOP A PLATFORM FOR EFFICIENT MULTIPLEX GENOME EDITING IN CATTLE GOAT AND SHEEP. WE WILL PURSUE THE FOLLOWING SPECIFIC OBJECTIVES: 1) TO OPTIMIZE CRISPR-CAS9 DELIVERY AND GENOME EDITING REAGENTS FOR EFFICIENT GENE DISRUPTION ALLELE INTROGRESSION AND TARGETED GENE INSERTION IN BOVINE OVINE AND CAPRINE ESCS; AND 2) TO OPTIMIZE CONDITIONS FOR MULTIPLEXED GENE EDITING IN CATTLE ESCS. OUTCOMES OF THIS PROPOSAL WILL INCLUDE STEM CELL-BASED PLATFORMS FOR RAPID AND EFFICIENT INTRODUCTION OF MULTIPLE GENOMIC MODIFICATIONS IN THREE IMPORTANT FOOD ANIMAL SPECIES.
$280,056University Of California, Davis · · FY2020 · National Institute of Food and Agriculture
Project 1:Targeting HIF2 and VHL Synthetic Lethal Interactions in Kidney Cancer
$279,906William G Kaelin · Beth Israel Deaconess Medical Center · P50 · FY2023 · CA
Understanding the Healthy Gut Virome and its Role in Human Health
$279,780Mark J Young · Montana State University - Bozeman · R01 · FY2016 · GM
Project II
$279,650John C Schimenti · Magee-Women'S Res Inst And Foundation · P50 · FY2025 · HD
A developmental engineering toolbox for large-scale tissue engineering
$279,481Alex Hughes · University Of Pennsylvania · R35 · FY2019 · GM
Tumor Microenvironment Determinants of TKI Response in Oncogene-Driven Lung Cancer
$279,465Erin L Schenk · University Of Colorado Denver · K08 · FY2025 · CA
Tumor Microenvironment Determinants of TKI Response in Oncogene-Driven Lung Cancer
$279,465Erin L Schenk · University Of Colorado Denver · K08 · FY2024 · CA
Ferroptosis resistance as a key driver in acquired radiation resistance
$279,167Boyi Gan · University Of Tx Md Anderson Can Ctr · U54 · FY2025 · CA
Ferroptosis resistance as a key driver in acquired radiation resistance
$279,166Boyi Gan · University Of Tx Md Anderson Can Ctr · U54 · FY2023 · CA