Sort
17,054 grants matching “genome editing”
GRAIN LEGUMES CONSTITUTE MAJOR SOURCES OF PLANT DIETARY PROTEIN FOR HUMAN AND FEED CONSUMPTION. CURRENTLY, GENERATING NOVEL TRAITS IN THESE CROPS IS LARGELY LIMITED TO CLASSICAL MUTAGENESIS AND GENOMICS-ASSISTED BREEDING METHODS. GENE-EDITING OFFERS GREAT POTENTIAL FOR ACCELERATING CROP IMPROVEMENT THROUGH EFFICIENT AND PRECISE GENETIC MODIFICATIONS. TWO KEY TECHNOLOGICAL BARRIERS, LACK OF EFFICIENT REAGENT DELIVERY METHODS AND POOR PLANT REGENERATION RATES, HAVE IMPEDED USE OF GENE EDITING IN THESE CROPS. AGROBACTERIUM-MEDIATED TRANSFORMATION HAS BEEN THE PREFERRED METHOD TO DELIVER GENE EDITING REAGENTS IN PLANT SPECIES, BUT HAS SHOWN LITTLE SUCCESS IN GRAIN LEGUMES DUE TO THE HOST RANGE LIMITATION AND STRONG GENOTYPE-DEPENDENCY. OUR LABORATORIES HAVE BEEN DEVELOPING DIRECT DELIVERY METHODS USING BIOLISTICS OR NANOPARTICLES TO OVERCOME THESE LIMITATIONS. EFFICIENT GENE EDITING HAS BEEN ACHIEVED BY DIRECTLY DELIVERING CRISPR/CAS9 DNA OR RIBONUCLEOPROTEINS VIA THE BIOLISTIC-BASED APPROACH. MOREOVER, WE RECENTLY DEVELOPED A NEW METHOD TO IMPROVE PLANT REGENERATION USING DEVELOPMENTAL REGULATORS (DRS), SUCH AS THE BABY BOOM (BBM) AND WUSCHEL (WUS) GENES, IN SOYBEAN. IN THIS PROPOSAL, WE AIM TO EXTEND OUR DIRECT DELIVERY AND IMPROVED PLANT REGENERATION METHODS TO TWO GRAIN LEGUMES, COMMON BEAN AND CHICKPEA. BY COMBINING THESE METHODS WITH CRISPR/CAS9 REAGENTS, WE WILL EXPAND THE USE OF GENETIC TRANSFORMATION AND GENE EDITING IN GRAIN LEGUMES.
$281,184Regents Of The University Of Minnesota · · FY2021 · National Institute of Food and Agriculture
Functional impact, mechanistic role, and targetability of ROS1 aberrations in cancer
$281,116Monika A Davare · Oregon Health & Science University · R01 · FY2024 · CA
Mapping endogenous protein dynamics in living cells
$280,502Bo Huang · University Of California, San Francisco · R01 · FY2020 · GM
Mapping endogenous protein dynamics in living cells
$280,502Bo Huang · University Of California, San Francisco · R01 · FY2021 · GM
Mapping endogenous protein dynamics in living cells
$280,502Bo Huang · University Of California, San Francisco · R01 · FY2022 · GM
Biological Core
$280,415Carolyn Ann Worby · University Of Kentucky · P01 · FY2016 · NS
Elucidating Mechanisms of Recognition and Excision of Damaged Bases by NEIL glycosylases
$280,388Sheila Sue David · University Of California At Davis · R01 · FY2021 · GM
THE DEVELOPMENT OF CRISPR-CAS9 AND RELATED TECHNOLOGIES FOR EDITING MAMMALIAN CELLS AND EMBRYOS HAS RESULTED IN UNPRECEDENTED OPPORTUNITIES FOR IMPROVING LIVESTOCK GENETICS. TO TAKE FULL ADVANTAGE OF GENE EDITING TECHNOLOGIES APPLIED TO ANIMALS SUCH AS INTROGRESSION OF ALLELES FOR IMPROVED GENETIC ADVANCE MULTIPLE EDITS ON THE SAME INDIVIDUAL ARE OFTEN REQUIRED WHICH CURRENTLY CONSTITUTES A TECHNOLOGICAL BARRIER FOR FOOD ANIMAL SPECIES. WE HAVE RECENTLY BEEN ABLE TO ISOLATE AND MAINTAIN STABLE BOVINE EMBRYONIC STEM CELLS (BESC) FULFILLING AN UNMET NEED FOR LIVESTOCK SPECIES THAT HAD BEEN PURSUED FOR THE LAST 30 YEARS. WE HAVE FURTHER OPTIMIZED THE CULTURE CONDITIONS FOR BOVINE ESCS AND EXTENDED THE TECHNOLOGY TO SHEEP AND GOAT SPECIES. ESCS REPRESENT AN IDEAL PLATFORM FOR INTRODUCING MULTIPLE GENETIC MODIFICATIONS AS THEY CAN BE CULTURED INDEFINITELY MAINTAIN GENOMIC STABILITY AND ARE AMENABLE TO SCNT/CLONING FOR PRODUCING ESC-DERIVED OFFSPRING. THE GOAL OF THE PROPOSED PROJECT IS TO DEVELOP A PLATFORM FOR EFFICIENT MULTIPLEX GENOME EDITING IN CATTLE GOAT AND SHEEP. WE WILL PURSUE THE FOLLOWING SPECIFIC OBJECTIVES: 1) TO OPTIMIZE CRISPR-CAS9 DELIVERY AND GENOME EDITING REAGENTS FOR EFFICIENT GENE DISRUPTION ALLELE INTROGRESSION AND TARGETED GENE INSERTION IN BOVINE OVINE AND CAPRINE ESCS; AND 2) TO OPTIMIZE CONDITIONS FOR MULTIPLEXED GENE EDITING IN CATTLE ESCS. OUTCOMES OF THIS PROPOSAL WILL INCLUDE STEM CELL-BASED PLATFORMS FOR RAPID AND EFFICIENT INTRODUCTION OF MULTIPLE GENOMIC MODIFICATIONS IN THREE IMPORTANT FOOD ANIMAL SPECIES.
$280,056University Of California, Davis · · FY2020 · National Institute of Food and Agriculture
A new tool for the cell-specific identification of RNA binding protein targets
$279,984Michael Rosbash · Brandeis University · R01 · FY2016 · DA
EAGER: Generating diploid gametes in maize
$279,922Venkatesan Sundaresan · University Of California-Davis · · FY2025 · BIO
Mitochondria mediated intercellular metabolic coupling in bone marrow regeneration
$279,840Jose A. Cancelas · Cincinnati Childrens Hosp Med Ctr · R01 · FY2020 · DK
Mitochondria mediated intercellular metabolic coupling in bone marrow regeneration
$279,840Jose A. Cancelas · Cincinnati Childrens Hosp Med Ctr · R01 · FY2022 · DK
Mitochondria mediated intercellular metabolic coupling in bone marrow regeneration
$279,840Jose A. Cancelas · Cincinnati Childrens Hosp Med Ctr · R01 · FY2021 · DK
Understanding the Healthy Gut Virome and its Role in Human Health
$279,780Mark J Young · Montana State University - Bozeman · R01 · FY2016 · GM
Cancer Center Administration
$279,704David M Gosky · Ohio State University · P30 · FY2024 · CA
Cancer Center Administration
$279,702David M Gosky · Ohio State University · P30 · FY2023 · CA
Cancer Center Administration
$279,702David M Gosky · Ohio State University · P30 · FY2021 · CA
Cancer Center Administration
$279,702David M Gosky · Ohio State University · P30 · FY2022 · CA
Genome Editing Testing Section
$279,537Cathleen M Lutz · Jackson Laboratory · U42 · FY2019 · OD
MECHANISM OF INTERFERON ACTION
$279,537University Of California Santa Barbara · R01 · FY2001 · AI
MECHANISM OF INTERFERON ACTION
$279,534University Of California Santa Barbara · R01 · FY2000 · AI
New tools for the characterization of gene predictions in prokaryote genomes: app
$279,470Luciano Brocchieri · University Of Florida · R01 · FY2011 · GM
Disease Models and Mechanisms Core
$279,288Stefan Somlo · Yale University · P30 · FY2019 · DK
Triggering Innate Immunity in Tumor Cells to Overcome Resistance to Immunotherapy
$279,077Jeffrey J. Ishizuka · Yale University · K08 · FY2021 · CA
Triggering Innate Immunity in Tumor Cells to Overcome Resistance to Immunotherapy
$279,077Jeffrey J. Ishizuka · Yale University · K08 · FY2020 · CA