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17,054 grants matching “genome editing”
Swine model of monogenic diabetes with potential implications for T1D, T2D, and double diabetes
$300,000Adrienne Leigh Watson · Recombinetics, Inc. · R43 · FY2019 · DK
CCR5 immunotoxins as components of HIV cure regimens
$300,000Dennis J Hartigan-O'Connor · Tendel Therapies Inc. · R41 · FY2023 · AI
Plug-and-Play HLA for Safer and Personalized Off-the-Shelf Cell Therapies using big-DNA technology
$300,000Leslie Mitchell · Neochromosome, Inc. · R43 · FY2021 · AI
Plug-and-Play HLA for Safer and Personalized Off-the-Shelf Cell Therapies using big-DNA technology
$300,000David Minh Truong · Neochromosome, Inc. · R43 · FY2020 · AI
BROAD-SCALE PRODUCTION OF TRANSGENIC PLANTS CAN MITIGATE THE URGENT NEED FOR SUSTAINABLE AND HIGH-YIELDING CROPS. LEVERAGING NANOMATERIALS BELOW THE PLANT CELL WALL SIZE EXCLUSION LIMIT TO CREATE PLANT GENETIC TRANSFORMANTS WILL ENABLE AN EFFICIENT METHOD FOR PRODUCING GENE EDITED NON-TRANSGENIC CROPS TO MEET OUR FOOD AND ENERGY NEEDS.THE PROPOSED STUDY WILL VALIDATE NANOMATERIALS AS A PASSIVE GENE AND PROTEIN DELIVERY TOOL IN PLANTS, AND WILL ENABLE EFFICIENT GENE EDITING OF PLANTS AND PLASTIDS WITHOUT TRANSGENIC DNAINTEGRATION. THE SPECIFIC AIMS OF THIS PROJECT INCLUDE OBJECTIVE 1: TO SYNTHESIZE AND CHARACTERIZE NANOMATERIALS AND DETERMINE THEIR FATE WHEN INTERFACED WITH PLANTS, OBJECTIVE 2: TESTING AND CONFIRMING TTHAT NANOMATERIALS CAN PRODUCE TRANSGENIC PLANTS, AND OBJECTIVE 3: DELIVERY OF GENOME EDITING DNA VECTORS BY WHICH TO PERMANENTLY EDIT THE PLANT GENOME. KNOWLEDGE GAINED FROM A SYSTEMATIC STUDY OF NANOPARTICLE-PLANT INTERACTIONS WILL ALSO INFORM ORTHOGONAL AREAS OF RESEARCH THAT STAND TO BENEFIT FROM NANOSCALE TOOLS FOR PLANT BIOLOGY: PLASTID AND PHOTOSYNTHESIS GENETIC ENGINEERING, ENVIRONMENTAL IMPACTS OF NANOMATERIALS, AND DEVELOPMENT OF NANOSCALE TOOLS TIME-CONTROLLED RELEASE AND INCREASED UPTAKE OF NUTRIENTS IN PLANTS. FURTHERMORE, SUCCESS IN RNP DELIVERY COULD PRODUCE A HIGH-EFFICIENCY AND SPECIES-INDEPENDENT PLATFORM WITH PASSIVE (NON-BIOLISTIC) DELIVERY BY WHICH TO GENETICALLY MODIFY PLANTS WITHOUT DNA.
$300,000Regents Of The University Of California, The · · FY2019 · National Institute of Food and Agriculture
BTT EAGER: INDUCTION OF DOUBLE HAPLOIDS IN WHEAT USING CENH3 MUTANTS AND GENOME EDITING
$299,999University Of California, Davis · · FY2019 · National Institute of Food and Agriculture
EAGER: A Transient System for Cassava Genome Editing
$299,999Linda K Hanley-Bowdoin · North Carolina State University · · FY2014 · BIO
EAGER: Development of a Novel Method for Rapid and Efficient Genome Editing in Tomatoes
$299,999Neelima R Sinha · University Of California-Davis · · FY2016 · BIO
EAGER: Biomanufacturing: Optimizing the Isolation, Transfection, and Expansion of CAR-T Cells with Modified PES Membranes
$299,999Jacob Elmer · Villanova University · · FY2016 · ENG
Parametric design software for nanostructured CRISPR payloads
$299,999Steven L Armentrout · Parabon Nanolabs, Inc. · R43 · FY2023 · GM
Attenuated malaria sporozoite vaccine using a P. falciparum blood-stage auxotroph
$299,998Stephen Lev Hoffman · Sanaria, Inc. · R43 · FY2013 · AI
Attenuated malaria sporozoite vaccine using a P. falciparum blood-stage auxotroph
$299,998Stephen Lev Hoffman · Sanaria, Inc. · R43 · FY2014 · AI
ReDDDoT Phase 1: Planning Grant: Piloting an impact accelerator model for cultivating equity and ethics in genetics innovation
$299,993Marnie E Gelbart · Harvard University · · FY2024 · TIP
Core 2: Therapeutic Viral Vector Design and Development Core
$299,992Louise R. Rodino-Klapac · Research Inst Nationwide Children'S Hosp · P50 · FY2016 · AR
NSF2026: EAGER: The evolution and diversity of the human brain
$299,990Richard E Green · University Of California-Santa Cruz · · FY2020 · SBE
EAGER: Lambda-Red-enhanced homologous recombination in plants
$299,988Jose M Alonso · North Carolina State University · · FY2019 · BIO
EAGER: SYNPLASOMES - SYNthetic PLAnt epiSOMES for plant genome editing and engineering
$299,988Bernie Wone · University Of South Dakota Main Campus · · FY2024 · BIO
THE CRISPR/CAS NUCLEASES HAVE BEEN USED FOR EFFICIENT TARGETED GENOME EDITING IN VARIOUS ORGANISMS INCLUDING PLANTS. MOST PUBLISHED REPORTS USE AGROBACTERIUM-MEDIATED TRANSGENIC APPROACH TO INTRODUCE GENOME EDITING COMPONENTS INTO PLANTS. PRODUCTION OF TRANSGENIC PLANTS IS TIME CONSUMING, LABOR-INTENSIVE, AND SOME IMPORTANT CROP PLANTS ARE RECALCITRANT TO TRANSFORMATION. THEREFORE, AN EFFICIENT SYSTEM TO DELIVER GENE EDITING COMPONENTS INTO PLANT CELLS THAT CIRCUMVENTS THE REQUIREMENT OF TRANSFORMATION IS REQUIRED. IN OBJECTIVE 1, WE WILL ENGINEER AN RNA VIRUS-BASED TOBACCO RATTLE VIRUS (TRV) VECTORS FOR DELIVERY OF GENE EDITING COMPONENTS INTO THE GROWING PARTS OF THE PLANTS TO INDUCE HIGH EFFICIENCY OF HERITABLE GENE EDITING. SINCE RNA VIRUSES DO NOT INTEGRATE INTO THE PLANT GENOME, THEY ARE NOT TRANSMITTED THROUGH GERMLINE TO THE NEXT GENERATION AND THEREFORE, THE INDUCED MUTATION WILL BE FREE OF TRANSGENES. IN OBJECTIVE 2, WE WILL USE THE OPTIMIZED TRV VECTOR DEVELOPED IN OBJECTIVE 1 TO DELIVER GENE EDITING COMPONENTS AND ESTABLISH THE EFFICIENCY OF GERMINAL TRANSMISSION OF MUTATIONS USING NICOTIANA BENTHAMIANA AND TOMATO PLANT MODEL SYSTEMS.CONSIDERING DELIVERY OF GENOME EDITING COMPONENTS IS A MAJOR BARRIER IN TERMS OF EFFECTIVE USE OF CRISPR TECHNOLOGY IN MANY MAJOR AND MINOR CROP PLANTS, THE SUCCESSFUL DEVELOPMENT OF TRV-BASED SYSTEM WILL HAVE A MAJOR IMPACT ON BREEDING AND CROP IMPROVEMENT PROGRAMS. FURTHERMORE, THE ENGINEERING PRINCIPLES ADAPTED FOR DEVELOPING TRV SYSTEM WILL BE USEFUL TO DEVELOP OTHER VIRUS-BASED VECTORS.
$299,976University Of California, Davis · · FY2020 · National Institute of Food and Agriculture
EAGER: Biomanufacturing: Development of a Quantitative Framework of Directed Stem Cell Differentiation in Scalable Bioreactors
$299,973Emmanuel S Tzanakakis · Tufts University · · FY2015 · ENG
EAGER: Development of tools for probing the role of DNA methylation in maize
$299,967Nathan Springer · University Of Minnesota-Twin Cities · · FY2018 · BIO
GENOME EDITING IS A REVOLUTIONARY TECHNOLOGY THAT ALLOW ONE TO MAKE PRECISE CHANGES IN A PLANT'S GENETIC BLUEPRINT FOR AGRICULTURE. HOWEVER, ITS APPLICATION TO MANY MINOR CROPS LIKE ALFALFA, GRAPEVINES, LEMONS, LIMES AND ORANGES, IS LIMITED. THE LIMITATION IN USING GENE-EDITING TECHNIQUES IN THESE CROPS IS MAINLY DUE TO THE INABILITY OF STEM CELLS OF THESE PLANTS TO REFORM WHOLE PLANTS IN TISSUE CULTURE. THERE ARE ONLY A VERY FEW VARIETIES OF ALFALFA, ORANGES, LIMES, LEMONS AND GRAPEVINES FOR WHICH WE ARE CAPABLE OF FORMING WHOLE PLANTS FROM CELLS IN TISSUE CULTURE AND THE FEW VARIETIES THAT WE HAVE BEEN SUCCESSFUL WITH DO SO AT A VERY LOW FREQUENCY.WE RECENTLY DISCOVERED THAT A GENE SEQUENCE ENCODING A CHIMERIC PROTEIN THAT COMPRISES A GROWTH REGULATING FACTOR (GRF) AND ITS COFACTOR GRF INTERACTING FACTOR (GIF) (HENCEFORTH GRF-GIF) DRAMATICALLY INCREASED THE ABILITY OF WHEAT STEM CELLS TO REFORM WHOLE PLANTS IN TISSUE CULTURE. WHEN COMBINED WITH THE GENE EDITING SYSTEM CRISPR-CAS9, WE FOUND THAT THIS TECHNOLOGY ALSO SIGNIFICANTLY INCREASES THE TOTAL NUMBER OF GENE-EDITED PLANTS WE CAN PRODUCE USING TISSUE CULTURE. A NOVEL FEATURE OF THIS TECHNOLOGY IS THAT ALTHOUGH THE CHIMERIC GENE ENHANCES REGENERATION IN YOUNG CELLS, THE CHIMERIC GENE IS TURNED OFF IN OLDER TISSUE SO THAT THE PLANT GROWS NORMALLY TO MATURITY. IN WHEAT, THE TECHNOLOGY ALSO ALLOWS US TO MAKE GENE EDITS IN PLANTS WITHOUT THE USE OF A PLANT SELECTABLE MARKER GENE. THE GRF-GIF SYSTEM STIMULATES THE CELLS THAT CONTAIN IT TO REFORM PLANTS ON TISSUE CULTURE PLATES THAT LACK CERTAIN GROWTH FACTORS NORMALLY ADDED TO THE CULTURE MEDIUM AND WHICH ARE REQUIRED FOR THE CELLS TO REFORM PLANTS IN A PETRI DISH. PLANT SELECTABLE MARKER GENES ARE USED TO ALLOW PLANT CELLS TO GROW ON COMPOUND ON WHICH THEY NORMALLY CANNOT GROW. THESE SELECTIVE AGENTS ALLOWS ONLY CELLS CONTAINING THE NEW GENE EDIT ALONG WITH THE PLANT SELECTABLE MARKER GENE TO REFORM WHOLE PLANTS IN THE PRESENCE OF THE SELECTIVE AGENT. PLANT SELECTABLE MARKER GENES CURRENTLY USED ARE OFTEN THOSE THAT CONFER RESISTANT TO ANTIBIOTICS OR HERBICIDES. ALTHOUGH REGULATORY AGENCIES HAVE APPROVED GENETICALLY MODIFIED PLANTS CONTAINING THESE GENES FOR COMMERCIALIZATION, THE PUBLIC WOULD PREFER NOT TO HAVE THESE GENES IN THEIR FOOD SUPPLY.PROMISING PRELIMINARY RESULTS IN CITRUS SUGGEST THAT THIS TECHNOLOGY MAY BE APPLICABLE TO MULTIPLE PLANT SPECIES. THEREFORE THE OVERALL GOAL OF THIS PROPOSAL IS TO USE THIS TECHNOLOGY TO EXPAND THE BENEFITS OF GENE EDITING TO MINOR CROPS BY INCREASE THE EFFICIENCY WITH WHICH PLANT CELLS REFORM PLANTS ACROSS MULTIPLE VARIETIES OF ALFALFA, CITRUS AND VITIS VINIFERA. IN ADDITION, WE WANT TO USE THE ABILITY OF THE GRF-GIF TECHNOLOGY TO REPLACE ANTIBIOTIC AND HERBICIDE PLANT CELL SELECTION SYSTEM. LASTLY, WE WANT TO COMBINE GRF-GIF TECHNOLOGY WITH GENOME EDITING FOR EACH OF THE CROP TO ENHANCE THE PRODUCTION OF GENE-EDITED PLANTS. THIS PROJECT WILL OPEN THE DOOR TO MULTIPLE MINOR CROPS TO BENEFIT FROM THE POWER OF GENOME EDITING TECHNOLOGIES
$299,963University Of California, Davis · · FY2020 · National Institute of Food and Agriculture
EAGER: Repair and Recombination of Mitochondrial DNA
$299,944James E Haber · Brandeis University · · FY2021 · BIO
Ambystoma Genetic Stock Center - Revision to Include Curation and Informatics Component
$299,828Stephen Randal Voss · University Of Kentucky · P40 · FY2024 · OD
A Biosinpired Genetically-Expressible Dual-Modality Peptide-Based Probe for Super-Resolution Correlative Light and Electron Microscopy
$299,812Kevin Michael Lewis · Photon Biosciences, Llc · R41 · FY2024 · GM
Project 4: Prognostic Significance and Therapeutic Potential of DROSHA Mutations in Wilms Tumor
$299,672James F Amatruda · Ut Southwestern Medical Center · P50 · FY2016 · CA