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17,054 grants matching “genome editing”
THE FALL ARMYWORM (FAW) IS A POLYPHAGOUS PEST THAT CAUSES ECONOMIC LOSSES TO MANY CROPS.VORACIOUS FEEDING, YEAR-ROUND BREEDING AND HIGHLY EFFICIENT DETOXIFICATION SYSTEM TO COPE WITHPLANT TOXINS AND INSECTICIDES MAKE THIS A MAJOR INTERNATIONAL PEST. THEREFORE, THERE IS A NEED TO STUDYTHE MOLECULAR BASIS OF BIOLOGICAL PROCESSES THAT MAKE THIS INSECT A SUCCESSFUL INVADER AND PEST. ONEOF THE REASONS FOR THE LACK OF FUNCTIONAL GENOMICS INFORMATION ON THIS INSECT IS ITS POOR RESPONSE TORNA INTERFERENCE (RNAI). THE CRISPR/CAS9 GENOME EDITING METHOD COULD HELP TO OVERCOMETHESE LIMITATIONS. PRELIMINARY EXPERIMENTS SHOWED THAT CRISPR/CAS9 SYSTEM WORKS IN FAW. THEMAJOR GOAL OF THIS PROPOSAL IS TO DEVELOP THE CRISPR/CAS9 SYSTEM FOR EFFICIENT KNOCKOUT OF GENESIN FAW. TO BE ABLE TO IDENTIFY MUTANTS IN G0, METHODS BASED ON MULTIPLE SGRNAS IN COMBINATIONWITH MARKER SGRNA WILL BE DEVELOPED. THE CRISPR/CAS9 SYSTEM WILL BE EMPLOYED TO IMPROVERNAI EFFICIENCY IN FAW BY MUTATING DSRNASE GENES AND BY EXPRESSING SID-1 DSRNA TRANSPORTERGENE FROM CAENORHABDITIS ELEGANS AND STAUFENC GENE FROM LEPTINOTARSA DECELIMINEATA, THE TWOGENES SHOWN TO BE REQUIRED FOR EFFICIENT RNAI IN NEMATODES AND BEETLES RESPECTIVELY. THE PROPOSEDRESEARCH WILL DEVELOP CRISPR/CAS9 AND RNAI METHODS THAT COULD HELP IN FUNCTIONAL GENOMICSRESEARCH LEADING TO THE DEVELOPMENT OF NOVEL AND EFFICIENT METHODS TO CONTROL FAW.
$445,702University Of Kentucky Research Foundation, The · · FY2019 · National Institute of Food and Agriculture
Positive and negative regulation of REC-8 cohesin during meiosis
$445,500Aaron F Severson · Cleveland State University · R15 · FY2021 · GM
Editing to Create and Correct Gene Variants
$445,449Alexander Marson · University Of California, San Francisco · P01 · FY2022 · AI
Phenotypic Assay Design and Development for Rare and Neglected Diseases
$445,362James Inglese · National Center For Advancing Translational Sciences · ZIA · FY2019 · TR
Mechanistic analysis and allellic genome editing of iPSC-derived dominant LCA model
$445,257Erik M Ullian · University Of California, San Francisco · R01 · FY2024 · EY
Editing to Create and Correct Gene Variants
$445,243Alexander Marson · University Of California, San Francisco · P01 · FY2023 · AI
Critical role of TCF-1 on the epigenetic identity of memory T cells
$445,194Golnaz Vahedi · University Of Pennsylvania · R01 · FY2019 · HL
Mitochondrial R-loop in sepsis-induced cardiomyopathy
$445,156Wei Huang · University Of Cincinnati · R01 · FY2023 · HL
Molecular Mechanisms of Dystrophin Expression in Ameliorated Phenotypes
$445,145Kevin M Flanigan · Research Inst Nationwide Children'S Hosp · R01 · FY2025 · NS
Modeling Alzheimerâs disease using directly reprogrammed isogenic neural cells
$445,140Yanhong Shi · Beckman Research Institute/City Of Hope · R01 · FY2020 · AG
Modeling Alzheimerâs disease using directly reprogrammed isogenic neural cells
$445,140Yanhong Shi · Beckman Research Institute/City Of Hope · R01 · FY2021 · AG
Modeling Alzheimerâs disease using directly reprogrammed isogenic neural cells
$445,140Yanhong Shi · Beckman Research Institute/City Of Hope · R01 · FY2019 · AG
Damage-Associated Molecular Patterns Driving Fibrosis Progression in Scleroderma
$445,133John Varga · University Of Michigan At Ann Arbor · R01 · FY2022 · AR
Context-specific and combinatorial genetic regulatory grammars in diabetes
$445,110Stephen Cj Parker · University Of Michigan At Ann Arbor · R01 · FY2018 · DK
Mutual reinforcement between somatic mutations and transcription factors in clonal hematopoiesis
$445,109Yun Huang · Texas A&M University Health Science Ctr · R01 · FY2023 · DK
Regulation of TLR trafficking for proper self versus non-self discrimination
$445,067Gregory M Barton · University Of California Berkeley · R01 · FY2021 · AI
Unlocking microglia targeting for neurotherapeutics
$445,000Mariko L. Bennett · Children'S Hosp Of Philadelphia · DP5 · FY2025 · OD
Unlocking microglia targeting for neurotherapeutics
$445,000Mariko L. Bennett · Children'S Hosp Of Philadelphia · DP5 · FY2024 · OD
Unlocking microglia targeting for neurotherapeutics
$445,000Mariko L. Bennett · Children'S Hosp Of Philadelphia · DP5 · FY2023 · OD
Development of Modular CRISPR Genome Editing Technologies and Tools
$444,971Channabasavaiah Gurumurthy · University Of Nebraska Medical Center · R35 · FY2021 · HG
Exploring the Impact of Base Deaminase Deregulation on Precancer Evolution
$444,952Catriona Helen Macleod Jamieson · University Of California, San Diego · R01 · FY2025 · CA
Project 3
$444,770Peng Jin · Emory University · P50 · FY2020 · HD
Structure and Relations of Proteins and Nucleic Acids
$444,752Peter Hans Von Hippel · University Of Oregon · R01 · FY2007 · GM
Activity-Dependent Regulation of CaMKII and Synaptic Plasticity
$444,634Leslie C. Griffith · Brandeis University · R37 · FY2025 · NS
Activity-Dependent Regulation of CaMKII and Synaptic Plasticity
$444,634Leslie C. Griffith · Brandeis University · R37 · FY2023 · NS