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17,828 grants matching “crispr”
Selectively Targeting Oncogenic NRAS in Cancer
$490,596Kevin M Shannon · University Of California, San Francisco · R01 · FY2024 · CA
Targeting RNA Splicing in Glioma
$490,568Shi-Yuan Cheng · Northwestern University At Chicago · R01 · FY2023 · NS
Targeting RNA Splicing in Glioma
$490,568Shi-Yuan Cheng · Northwestern University At Chicago · R01 · FY2022 · NS
Targeting RNA Splicing in Glioma
$490,568Shi-Yuan Cheng · Northwestern University At Chicago · R01 · FY2025 · NS
Off target mechanisms of kinase inhibitor toxicities
$490,462Navjot Pabla · Ohio State University · R01 · FY2025 · DK
Mechanisms of Revertant Mosaicism in Ichthyosis with Confetti
$490,450Keith A Choate · Yale University · R01 · FY2021 · AR
Identification of metabolic regulators of glycerolipid synthesis and storage
$490,172Kivanc Birsoy · Rockefeller University · R01 · FY2022 · DK
Molecular characterization of a novel cause of human FSGS
$490,056Alda Tufro · Yale University · R01 · FY2020 · DK
Molecular characterization of a novel cause of human FSGS
$490,056Alda Tufro · Yale University · R01 · FY2018 · DK
EDGE CMT: Dissecting complex traits in wild isolates of yeast by high-throughput genome editing
$490,005Lars M Steinmetz · Stanford University · R01 · FY2024 · HG
The role of PHF6 in the control of hematopoietic stem cell aging.
$490,000Teresa Palomero · Columbia University Health Sciences · R01 · FY2025 · AG
EDGE CMT: Dissecting complex traits in wild isolates of yeast by high-throughput genome editing
$490,000Lars M Steinmetz · Stanford University · R01 · FY2025 · HG
**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** WHEAT BREEDING IN THE US GREAT PLAINS IS MOSTLY FOCUSED ON IMPROVING HARD WINTER WHEAT, WHICH REMAINS ONE OF THE MAJOR CROPS IN KANSAS, WITH 8-12 MILLION ACRES PLANTED EVERY YEAR AND ANNUAL PRODUCTION RANGING BETWEEN 280 AND 460 MILLION BUSHELS. GRAIN YIELD AND GRAIN QUALITY ARE AMONG THE MAJOR BREEDING TARGETS FOR HARD WINTER WHEAT. WITH CONTINUED DECREASE IN PLANTED WINTER WHEAT ACRES IN KANSAS, ABILITY TO HARVEST MORE GRAIN WITH INCREASED PROTEIN CONTENT AND BREAD MAKING QUALITY FROM SMALLER AREA ARE IMPORTANT FACTORS CONSIDERED BY WHEAT GROWERS DURING VARIETY SELECTION. IMPROVING GRAIN YIELD AND QUALITY IS A ALSO A MAIN PRIORITY FOR THE STATE'S COMMODITY ASSOCIATION AND MILLING INDUSTRY.RECENT ADVANCES IN WHEAT GENETICS AND GENOMICS HELPED TO BETTER UNDERSTAND THE GENETIC BASIS OF MANY AGRONOMIC TRAITS ALLOWING WHEAT BREEDERS TO ACCELERATE DEVELOPMENT OF NEW VARIETIES BY UTILIZING THIS INFORMATION. THE IDENTIFICATION OF GENES THAT UNDERLIE TRAIT VARIATION OPENED NEW OPPORTUNITIES FOR WHEAT IMPROVEMENT BY STACKING FAVOURABLE VARIANTS OF VARIOUS GENES, WHICH ARE THE PART OF THE BIOLOGICAL PATHWAYS CONTROLLING THE SAME OR COMPLEMENTARY AGRONOMIC TRAITS. THE SUCCESS OF THIS BREEDING PROCESS DEPENDENT ON THE AVAILABILITY OF NOVEL VARIANTS OF GENES. WHILE TRADITIONALLY THESE VARIANTS ARE INTRODUCED INTO THE BREEDING PROGRAMS FROM CULTIVARS FROM OTHER GEOGRAPHIC REGIONS, OR CLOSE AND DISTANT ANCESTORS OF WHEAT, RECENT ADVANCES IN GENOMIC TECHNOLOGIES PROVIDE AN OPPORTUNITY TO CREATE NOVEL GENE VARIANTS USING THE CRISPR TOOLS. THESE TOOLS ALLOW RESEARCHERS TO PRECISELY MODIFY THE GENETIC CODE AND CREATE NEW GENES THAT HAVE POTENTIAL TO IMPROVE AGRONOMIC TRAITS. RECOGNIZING THE FUTURE POTENTIAL OF THIS NOVEL GENE EDITING TECHNOLOGIES, KANSAS WHEAT COMMISSION AND THE STATE'S WHEAT GROWERS PRIORITIZED THIS DIRECTION OF RESEARCH, AND CONTRIBUTED FUNDING TO BUILD WHEAT GENOME EDITING CAPACITY AT K-STATE. OUR PROJECT WILL BUILD ON THESE RESOURCES AND EXPAND FURTHER THE SET OF TOOLS AVAILABLE FOR WHEAT GENOME EDITING.WE WILL FOCUS ON TRANSFERRING THE CRISPR-INDUCED GENES THAT SHOWED POTENTIAL TO INCREASE GRAIN YIELD AND GRAIN QUALITY INTO THE HARD WINTER WHEAT CULTIVARS FROM THE KANSAS WHEAT BREEDING PROGRAM. WE WILL INTRODUCE THE CRISPR-INDUCED GENES INTO THE WHEAT CULTIVARS THAT ALREADY HAVE OTHER SUPERIOR TRAITS, SUCH AS IMPROVED ROOT SYSTEM, GOOD BREAD MAKING QUALITY, HIGH YIELD, OR HIGH GRAIN PROTEIN CONTENT. BY TRANSFERRING THESE NEW VARIANTS OF GENES INTO THE ADAPTED CULTIVARS, WE WILL BE ABLE TO INVESTIGATE THEIR INTERACTION WITH GENES THAT DEFINE THE SUPERIOR TRAITS IMPORTANT FOR WHEAT PRODUCTION IN KANSAS, AND SELECT OPTIMAL COMBINATIONS OF CULTIVARS AND CRISPR-INDUCED GENES FOR IMPROVING WINTER WHEAT. THE CRISPR TOOLS PREVIOUSLY APPLIED TO EDIT THE WHEAT GENOME ARE NOT EFFECTIVE AT PERFORMING CERTAIN TYPES OF MANIPULATIONS WITH THE GENETIC CODE, SUCH AS REPLACEMENT OF INDIVIDUAL BASES IN DNA. IN OUR PROJECT, WE WILL TRY TO EXPAND THE RANGE OF DNA ALTERATIONS POSSIBLE IN THE WHEAT GENOME BY APPLYING NOVEL TECHNOLOGY, REFERRED TO AS PRIME EDITING, TO MODIFY GENES THAT HAVE POTENTIAL TO IMPROVE WHEAT PRODUCTIVITY.OUR PROJECT WILL COLLABORATE WITH THE WHEAT RESEARCH AND IMPROVEMENT PROGRAMS AT KSU, KANSAS WHEAT INNOVATION CENTER, INTERNATIONAL WHEAT YIELD PARTNERSHIP (IWYP), USDA NIFA WHEAT COORDINATED AGRICULTURAL PROJECT (CAP), AND THE NIFA IWYP WINTER WHEAT BREEDING INNOVATION (WWBI) HUB AT KSU. ALIGNING OUR PROJECT PRIORITIES WITH THE PRIORITIES OF THESE NATIONAL AND INTERNATIONAL INITIATIVES, WE WILL BROADEN THE PROJECT'S IMPACT, AND FACILITATE INCORPORATION OF THE PROJECT OUTCOMES INTO THE BREEDING PIPELINES OF PRIVATE AND PUBLIC BREEDING PROGRAMS. WE WILL WORK ON BROADENING THE TRAINING OPPORTUNITIES IN GENOME EDITING TECHNOLOGIES FOR GRADUATE STUDENTS, RESEARCHERS AND BREEDERS. OUR EFFORTS WILL CONTRIBUTE TO THE KANSAS WHEAT BREEDING PROGRAM'S MISSION TO DEVELOP STRATEGIES FOR DELIVERING HIGH-YIELDING WHEAT VARIETIES WITH IMPROVED QUALITY TO KANSAS FRAMERS, AND ADDRESS ONE OF THE KEY PRIORITIES OF THE NIFA USDA PROGRAM TO IMPROVE CROP PRODUCTIVITY AND QUALITY IN THE LOCAL U.S. FARMING SYSTEMS.
$489,999Kansas State University · · FY2021 · National Institute of Food and Agriculture
Image-guided radiation-induced permeability (IGRIP) for IGDD
$489,602Stephen J. Kron · University Of Chicago · R01 · FY2018 · CA
The role of long noncoding RNA CRNDE in normal physiology and cancer
$489,568Joshua T Mendell · Ut Southwestern Medical Center · R01 · FY2023 · CA
The role of long noncoding RNA CRNDE in normal physiology and cancer
$489,566Joshua T Mendell · Ut Southwestern Medical Center · R01 · FY2025 · CA
Mechanisms of Variant ER-alpha Function in Breast Cancer
$489,550Amy Fowler · University Of Wisconsin-Madison · R01 · FY2025 · CA
Functional dissection of fibronectin type 3 domains of SORL1 in Alzheimers disease associated microglia
$489,509Falak Sher · Columbia University Health Sciences · R01 · FY2021 · AG
Human cellular models of TB and innate immunity
$489,500Anne Goldfeld · Boston Children'S Hospital · R21 · FY2025 · AI
Functional Analysis of Variants Underlying T Cell Defects
$489,499David L Wiest · University Of California, San Francisco · P01 · FY2020 · AI
FATTY ACID ESTERS, MANY OF WHICH HAVE FRUITY FLAVORS AND FRAGRANCES, ARE VALUABLE CHEMICALS WITH EXTENSIVE APPLICATIONS IN FOOD, BEVERAGE, COSMETICS, AND PHARMACEUTICAL INDUSTRIES. SHORT-CHAIN ESTERS CAN ALSO BE USED FOR SOLVENTS, COATINGS, PAINTS, AND FUEL COMPONENTS. IT IS PROJECTED THAT THE US MARKET DEMAND FOR FATTY ACID ESTERS WILL REACH $4.34 BILLION BY 2022. WHILE THE TRADITIONAL APPROACHES FOR ESTER PRODUCTION THROUGH PETROLEUM CHEMICAL ROUTES ARE HIGHLY ENERGY-CONSUMING AND GENERATE A LARGE AMOUNT OF ENVIRONMENTAL POLLUTANTS, MORE AND MORE INTERESTS HAVE BEEN EVOKED RECENTLY FOR ESTER PRODUCTION THROUGH BIOLOGICAL ROUTES. THERE ARE GENERALLY TWO MICROBIAL PATHWAYS FOR ESTER PRODUCTION, EITHER BY ALCOHOL ACYL TRANSFERASES (AATS) WITH ACYL-COA AND ALCOHOLS AS FEEDING COMPOUNDS, OR BY LIPASE WITH FATTY ACID AND ALCOHOL AS REACTING MATERIALS. NON-PATHOGENIC CLOSTRIDIUM HAS TREMENDOUS SIGNIFICANCE FOR INDUSTRIAL BIOCHEMICAL PRODUCTION. WITH THE ANAEROBIC FERMENTATION PATHWAY, IT CAN GENERATE ACYL-COAS (ACETYL-COA AND BUTYL-COA), ACIDS (ACETIC ACID AND BUTYRIC ACID), AND ALCOHOLS (ETHANOL AND BUTANOL). THEREFORE, IN THIS PROJECT, WE PROPOSE TO USE CLOSTRIDIUM AS A PLATFORM TO BE METABOLICALLY ENGINEERED FOR ESTER PRODUCTION, PARTICULARLY FOR BUTYL BUTYRATE (BB) PRODUCTION. FIRST, BASED ON THE CRISPR-CAS SYSTEM THAT WE RECENTLY DEVELOPED FOR GENOME ENGINEERING, WE WILL ENGINEER THE CLOSTRIDIUM STRAIN FOR ENHANCED BUTYRYL-COA/BUTYRATE AND BUTANOL CO-PRODUCTION, PROVIDING FEEDING COMPOUNDS FOR BB PRODUCTION. FURTHER, WE WILL ENGINEER THE MODIFIED STRAIN FOR BB PRODUCTION, BY INTRODUCING LIPASE GENES (PRODUCING BB FROM BUTYRIC ACID AND BUTANOL) AND AAT GENES (PRODUCING BB FROM BUTYRYL-COA AND BUTANOL). MEANWHILE, WE WILL DEVELOP A GENOME-SCALE METABOLIC MODEL, TO GUIDE OUR FURTHER METABOLIC OPTIMIZATION FOR ENHANCED BB PRODUCTION. ULTIMATELY, WE WILL ESTABLISH A BIOPROCESS BASED ON THE METABOLICALLY STABLE CLOSTRIDIUM STRAINS FOR RENEWABLE ESTER PRODUCTION FROM LOW-VALUE CARBON SOURCES.THIS PROJECT AIMS TO TACKLE A KEY ISSUE RELATED TO BIOFUEL/BIOCHEMICAL PRODUCTION BY PRODUCING A HIGH VALUE BIOPRODUCT THAT IS EASILY RECOVERABLE, THUS DECREASING ENDPRODUCTS TOXICITY AND IMPROVING FEEDSTOCK CONVERSION EFFICIENCY AND PRODUCT PRODUCTION RATE. THIS RESEARCH ALIGNS WELL WITH THE PRIORITY AREA OF BIOPROCESSING AND BIOENGINEERING. VALUE-ADDED BB WILL BE PRODUCED FROM LOW-VALUE MATERIALS THROUGH OPTIMIZED BIOPROCESS USING ENGINEERED MICROORGANISM. THIS REPRESENTS A POTENTIALLY TRANSFORMATIVE RESEARCH WHICH CAN LEAD TO AN ENABLING BIOPROCESS IN SUPPORT OF THE US BIOECONOMY. IT ALIGNS WELL WITH THE US ENDEAVORS TO FOSTER THE PRODUCTION CAPACITY OF BIOFUELS, BIOENERGY, AND BIOPRODUCTS FROM LOW-VALUE MATERIALS.
$489,406Auburn University · · FY2018 · National Institute of Food and Agriculture
Ubiquitinâmediated proteolysis and cell cycle control
$489,225Jeffrey Wade Harper · Harvard Medical School · R01 · FY2024 · AG
Function of Wdfy4 in cross-presentation and immunity
$489,211Kenneth M. Murphy · Washington University · R01 · FY2023 · AI
Systems Immunogenetics of Influenza Virus Infection in the Collaborative Cross
$489,101Mark T Heise · Univ Of North Carolina Chapel Hill · U19 · FY2018 · AI
Defining cellular receptors for the Bacillus cereus hemolysin BL toxin (HBL) and the development of anti-HBL therapies
$489,084Shihui Liu · University Of Pittsburgh At Pittsburgh · R01 · FY2020 · AI