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17,054 grants matching “genome editing”
Characterizing patient-specific TBR1 mutations: Understanding a master regulator of autism risk.
$499,244Brian James O'Roak · Oregon Health & Science University · R01 · FY2019 · MH
Characterizing patient-specific TBR1 mutations: Understanding a master regulator of autism risk.
$499,244Brian James O'Roak · Oregon Health & Science University · R01 · FY2020 · MH
Guide RNA Binding Complex
$499,231Ruslan Aphasizhev · Boston University Medical Campus · R01 · FY2021 · AI
Pre-clinical development of SPY-DYS45-55, a CRISPR/Cas9 platform for Duchenne muscular dystrophy
$499,207Courtney S Young · Myogene Bio, Llc · R44 · FY2022 · AR
THIS RESEARCH ADDRESSES AREA 5A OF THE USDA BIOTECHNOLOGY RISK ASSESSMENT CALL FOR PROPOSALS, INCLUDING RESEARCH ADDRESSING THE UNINTENDED EFFECTS IN BIOENGINEERED ORGANISMS DEVELOPED USING GENOME EDITING TECHNOLOGY OR OTHER ENGINEERING TECHNIQUES.ADVANCES IN BIOLOGY AND GENE EDITING METHODS ARE ENABLING THE ADVANCED MODIFICATIONS OF GENOMES IN A WIDE VARIETY OF CROP SPECIES. GENES THAT CONTROL SHOOT DEVELOPMENT ARE POWERFUL TOOLS FOR HELPING GENE TRANSFER AND EDITING IN MANY DIFFICULT CROP VARIETIES, BUT USUALLY MUST BE REMOVED (EXCISED) FROM THE GENOME TO ENABLE NORMAL PLANT REGENERATION AND FERTILITY. IN VEGETATIVELY PROPAGATED CROPS, GENE EDITING AGENTS SUCH AS CRISPR CANNOT BE REMOVED DURING SEXUAL REPRODUCTION AS IS COMMON FOR ANNUAL CROPS, THUS ALSO BENEFIT FROM EXCISION METHODS. THE MOST COMMON TOOL FOR EXCISION IS THE CRE RECOMBINASE, HOWEVER, CRE EXPRESSION MAY IMPART UNDESIRABLE AND SUBSTANTIAL UNINTENDED GENOME MODIFICATIONS.USING POPLAR AS A TEST DICOT CROP, WE PROPOSE TO: 1) COMPLETE THE GENERATION AND TESTING OF A SYSTEM FOR CONTROLLED EXPRESSION OF CRE FOR TRANSGENE AND CRISPR-CAS REMOVAL IN CLONALLY PROPAGATED DICOT CROPS; 2) OPTIMIZE THE SYSTEM WITH RESPECT TO ITS CAPACITY TO PRODUCE AN EFFICIENT RATE OF EDITING AND EXCISION WITH MINIMAL CHIMERISM; 3) PRODUCE PROTOTYPE EDITED AND EXCISED REGENERANTS, AND ANALYZE EFFECTS OF CRE ACTIVITY ON VARIATION IN PLANT BIOMASS PRODUCTIVITY IN THE GREENHOUSE, GENOME-SCALE CHROMOSOME AND DNA BASE PAIR VARIATION, AND NEAR TO THE INSERTION/EXCISION SITE IMPACTS ON GENE EXPRESSION AND CHROMOSOME STRUCTURE. THIS WORK WILL HELP TO INFORM REGULATORS OF THE DEGREE TO WHICH THEY SHOULD BE CONCERNED WITH OFF-TARGET IMPACTS WHEN CRE RECOMBINASES ARE USED FOR GENOME EDITING.
$498,991Oregon State University · · FY2020 · National Institute of Food and Agriculture
Myosin Gene Diversity and Funciton
$498,723Leslie Anne Leinwand · University Of Colorado · R01 · FY2016 · GM
Natural Killer Cell Tolerance to Self
$498,667Wayne M. Yokoyama · Washington University · R01 · FY2021 · AI
Unraveling mechanisms of tumor suppression in lung cancer
$498,341Monte Meier Winslow · Stanford University · R01 · FY2020 · CA
Signaling mechanisms that detect stress and maintain homeostasis
$498,332Thomas Keith Blackwell · Joslin Diabetes Center · R35 · FY2020 · GM
Modulation of Viral Antigen Presentation in the Lung
$498,316Anna Karolina Palucka · Jackson Laboratory · U19 · FY2021 · AI
Connectivity and Function of the Asymmetric Habenulo-Interpeduncular Pathway
$498,270Marnie E Halpern · Dartmouth College · R37 · FY2024 · HD
Pathogenic Mechanisms of Craniometaphyseal Dysplasia
$498,194I-Ping Chen · University Of Connecticut Sch Of Med/Dnt · R01 · FY2025 · DE
The novel smooth muscle-specific lncRNA CARMN is a critical regulator of smooth muscle phenotype
$498,063Jiliang Zhou · Augusta University · R01 · FY2020 · HL
Investigating the role of a novel fungal RNA in intestinal innate immunity and inflammation
$498,000Xin Li · Ut Southwestern Medical Center · DP2 · FY2025 · AI
Genetically-encoded molecular imaging of genome-edited immune cells
$497,870Nalinikanth Kotagiri · University Of Cincinnati · R01 · FY2024 · CA
New Targets for Reproductive Control of Mosquito Vectors
$497,864Laura C Harrington · Cornell University · R01 · FY2018 · AI
Identification of Enhancers of Therapeutic Exon Skipping for DMD
$497,847M Carrie Miceli · University Of California Los Angeles · RC1 · FY2009 · AR
Selective pressures from inherited variation impacting myeloproliferative neoplasm initiation
$497,838Vijay Ganesh Sankaran · Boston Children'S Hospital · R01 · FY2023 · CA
Identification of Enhancers of Therapeutic Exon Skipping for DMD
$497,653M Carrie Miceli · University Of California Los Angeles · RC1 · FY2010 · AR
Foxp2 genetic control of sex differences in amygdala-driven social behavior
$497,652Joshua G Corbin · Children'S Research Institute · R21 · FY2024 · MH
Pharmacological and dietary inhibition of a novel metabolic-epigenetic crosstalk in head and neck cancer
$497,650De-Chen Lin · University Of Southern California · R01 · FY2025 · DE
RNA editing in islet function and inflammation
$497,550Alvin Carter Powers · Vanderbilt University Medical Center · R01 · FY2024 · DK
Identification and Functional Evaluation of Autosomal Recessive Nonsyndromic Hearing Impairment Genes in sub-Saharan Africans
$497,523Suzanne Margaret Leal · Baylor College Of Medicine · R01 · FY2018 · DC
uORF-mediated Translational Control of Cardiac Transcription Factor Expression
$497,488Peng Yao · University Of Rochester · R01 · FY2024 · HL
Pharmacological and dietary inhibition of a novel metabolic-epigenetic crosstalk in head and neck cancer
$497,275De-Chen Lin · University Of Southern California · R01 · FY2024 · DE