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17,054 grants matching “genome editing”
Research Resources for Model Amphibians
$499,886Stephen Randal Voss · University Of Kentucky · R24 · FY2022 · OD
Collaborative Research: EDGE FGT: Functional Genomic Tools for Parasitic Nematodes and their Bacterial Symbionts
$499,872Paul W Sternberg · California Institute Of Technology · · FY2021 · BIO
THE YIELD AND QUALITY OF FISH FILLETS ARE DETERMINED BY CELLULAR PROCESSES THAT GOVERN HOW MUSCLE TISSUE GROWS AND DEVELOPS. THE ACTIVIN/MYOSTATIN CELLULAR SIGNALING PATHWAY IS ONE OF THE MOST IMPORTANT MUSCLE GROWTH PATHWAYS IN VERTEBRATES AND WORKS BY DIRECTING MUSCLE FIBERS TO LIMIT THEIR OVERALL SIZE. DISRUPTION OF THE PATHWAY LEADS TO LARGE INCREASES IN MUSCLE MASS IN MAMMALS AND FISH. WHILE THE SIGNALING PROTEIN MYOSTATIN IS A WELL-ESTABLISHED ACTIVATOR OF THIS PATHWAY, IT DOES NOT ACT ALONE. IN MAMMALS, ACTIVIN A HAS AN EQUAL AND OVERLAPPING FUNCTION WITH MYOSTATIN TO SLOW MUSCLE GROWTH. OTHER THAN MYOSTATIN, THE FUNCTION OF THE BROADER ACTIVIN/MYOSTATIN SIGNALING PATHWAY IN FISH IS POORLY UNDERSTOOD. WE HYPOTHESIZE THAT ACTIVIN A HAS AN ESSENTIAL ROLE IN CONTROLLING THE SIZE OF MUSCLE TISSUE IN FISH AND THAT THERE ARE SPECIFIC MECHANISMS BY WHICH THE PATHWAY COORDINATES SIGNALING ACROSS MUSCLE AND NON-MUSCLE TISSUES. TO ADDRESS THESE HYPOTHESES, THE RESEARCH WILL FIRST ESTABLISH A COMPREHENSIVE ATLAS OF THE EXPRESSION OF ALL ACTIVIN/MYOSTATIN SIGNALING PATHWAY MEMBERS ACROSS 11 TISSUES AND 12 MUSCLE DEVELOPMENTAL STAGES, USING COMMERCIAL RAINBOW TROUT (RBT) AS A MODEL SYSTEM. CRISPR GENOME EDITING TECHNOLOGY WILL ALSO BE USED TO DISRUPT THE FUNCTION OF ALL FOUR ACTIVIN A GENES IN RBT AND INVESTIGATE WHETHER LOSS OF ACTIVIN A SIGNALING RESULTS IN AN INCREASE IN MUSCLE SIZE IN FISH. THESE GENE-EDITED LINES WILL BE PRODUCED SOLELY AS A BASIC RESEARCH MODEL SYSTEM AND ARE EXPECTED TO PROVIDE A WEALTH OF KNOWLEDGE ON THE PHYSIOLOGICAL FUNCTION OF ACTIVIN A IN FISH. BY DEVELOPING A DETAILED GENE EXPRESSION ATLAS FOR THE PATHWAY, THE RESEARCH WILL CREATE AN IMPORTANT RESOURCE TO THE SCIENTIFIC COMMUNITY, THE FIRST TO BE ESTABLISHED FOR ANY SPECIES TO DATE. THE IMPACT OF THE PROPOSED BASIC RESEARCH WILL FACILITATE A DEEPER UNDERSTANDING OF PERHAPS THE MOST IMPORTANT MUSCLE GROWTH PATHWAY IN FISH. THIS WILL PROVIDE THE FOUNDATION FOR EXPLOITING THE PATHWAY TO IMPROVE THE OVERALL YIELD AND QUALITY FISH FILLETS IN UNITED STATES FINFISH AQUACULTURE.
$499,859Washington State University · · FY2021 · National Institute of Food and Agriculture
Lacritin Regulation of Homeostasis and Ocular Surface Health
$499,831Gordon William Laurie · University Of Virginia · R01 · FY2023 · EY
Lacritin Regulation of Homeostasis and Ocular Surface Health
$499,831Gordon William Laurie · University Of Virginia · R01 · FY2024 · EY
Guide RNA Binding Complex
$499,707Ruslan Aphasizhev · Boston University Medical Campus · R01 · FY2020 · AI
TRI-PARTITE COLLABORATIVE: TARGETED GENOME EDITING TO UNDERSTAND AND ENHANCE GENETIC RESISTANCE TO BOVINE TUBERCULOSIS IN DOMESTIC CATTLE POPULATIONS (TARGET-TB)
$499,700Recombinetics Inc · · FY2018 · National Institute of Food and Agriculture
CD8+ T Cell Immunity to Cytomegalovirus
$499,674Stanley R Riddell · Fred Hutchinson Cancer Research Center · R01 · FY2011 · AI
GENOME-WIDE ASSESSMENT OF OFF-TARGET EFFECT AND REMOVAL OF TRANSGENES ASSOCIATED WITH TALEN-BASED GENE EDITING IN PLANT
$499,664Iowa State University Of Science And Technology · · FY2013 · National Institute of Food and Agriculture
GENOME EDITING TO CREATE GERM CELL DEFICIENT LIVESTOCK
$499,621University Of Maryland, College Park · · FY2018 · National Institute of Food and Agriculture
ADVANCING GENETICALLY ENGINEERED PLANTS FROM THE LAB TO THE FIELD AND EVEN THE EVENTUAL LARGE-SCALE PRODUCTION, CAN BE HAMPERED BY THE REGULATORY PROCESS BECAUSE OF THE COSTS INCURRED DURING THE LENGTHY PROCESS. THE NECESSARY REGULATORY COSTS, WHICH ALSO INCLUDE THOSE RELATED TO EXTENSIVE TESTING, MAKE IT LESS LIKELY THAT INNOVATIVE APPROACHES FOR CROP IMPROVEMENT DEVELOPED BY THE PUBLIC SECTOR AND SMALL BIOTECHNOLOGY COMPANIES WILL BE REALIZED. AS A RESULT, ANY NEW GENETICALLY MODIFIED (GM) CROPS ARE MORE LIKELY TO BE PRODUCED BY LARGE MULTINATIONAL COMPANIES, AND EVEN THEN, IT IS MAINLY LIMITED TO CROPS SUCH AS MAIZE AND SOYBEAN THAT HAVE LARGE GLOBAL MARKET VALUES. THE CRISPR (CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS)-CAS9 BASE EDITING SYSTEMS ARE POISED TO REVOLUTIONIZE MODERN CROP BREEDING DUE TO THEIR PROMISING APPLICATIONS FOR GENERATING TRANSGENE-FREE CROPS WITH QUANTITATIVE TRAITS INTRODUCED AT BASE PRECISION AT THE DNA LEVEL. BASE EDITING TECHNOLOGY PROVIDESAN EFFICIENT AND INEXPENSIVE WAY FORWARD FOR CROP BREEDING COMPARED TO CONVENTIONAL BREEDING METHODS. THIS PROJECT IS TO ADDRESS AN IMPORTANT KNOWLEDGE GAP, WHICH IS THE SAFETY OF PLANT BASE EDITING SYSTEMS. WE WILL FIRST IMPROVE THE EXISTING CRISPR-CAS9 C TO T AND A TO G BASE EDITING SYSTEMS FOR HIGHER EFFICIENCY AND ALTERED EDITING WINDOWS. NEXT, WE WILL GENERATE BASE-EDITED LINES OF TWO IMPORTANT FOOD CROPS, RICE AND TOMATO, AND USE WHOLE-GENOME SEQUENCING TO ASSESS UNINTENDED OFF-TARGET EFFECTS. IN PARALLEL, WE WILL ALSO SEQUENCE CONTROL LINES RECOVERED THROUGH THE PLANT REGENERATION AND TRANSFORMATION PHASES TO DETERMINE THE INCIDENCE OF UNINTENDED AFFECTS THAT CAN RESULT FROM SOMACLONAL VARIATION. THE RELEVANCE OF THE PROPOSED PROJECT TO THE BRAG PROGRAM IS VERY SIGNIFICANT BECAUSE OUR GOALS ARE CLOSELY ALIGNED WITH THE MISSION, IN THAT THE RESULTS WILL PROVIDE INFORMATION ACCESSIBLE BY FEDERAL REGULATORY AGENCIES TO CONSIDER DURING EVALUATION FOR INTRODUCTION OF CRISPR-CAS GENERATED ORGANISMS INTO THE ENVIRONMENT. THE KNOWLEDGE GENERATED FROM OUR WORK WILL GREATLY AID PLANT SCIENTISTS PRACTICING BASE EDITING TECHNOLOGY AS WELL AS FACILITATE THE DECISION-MAKING PROCESS AT REGULATORY AGENCIES SUCH AS USDA AND FDA. THIS RESEARCH PROJECT IS TO ADDRESS THE BRAG PROGRAM'S AREA 5G, WHERE IT CALLS FOR COMPARISON OF THE TYPES AND FREQUENCIES OF NUCLEIC ACID CHANGES INTRODUCED INTO IMPORTANT CROP GENOMES, VIA GENETIC ENGINEERING TECHNOLOGIES VERSUS OTHER PLANT BREEDING TECHNIQUES.
$499,607University Of Maryland, College Park · · FY2018 · National Institute of Food and Agriculture
Epigenetic regulation of Cdk5 in cognition and emotion
$499,571Elizabeth A Heller · University Of Pennsylvania · R01 · FY2023 · MH
COLLABORATIVE RESEARCH: The role of quality control in microbial translation
$499,563Michael Ibba · Ohio State University, The · · FY2014 · BIO
RNA editing of Ion Channels and Pumps
$499,562Miguel Holmgren · National Institute Of Neurological Disorders And Stroke · ZIA · FY2016 · NS
Systems Immunogenetics of Influenza Virus Infection in the Collaborative Cross
$499,496Mark T Heise · Univ Of North Carolina Chapel Hill · U19 · FY2019 · AI
Charcot-Marie-Tooth Disease Type 2E: Mechanism and therapy
$499,452Anthony Brown · Ohio State University · R01 · FY2025 · NS
Project 3: UW-CNOF Biological Validation Development
$499,429Christine M. Disteche · University Of Washington · U54 · FY2015 · DK
WITH THE ADVANCES OF GENE EDITING TECHNOLOGIES, IT IS NOW POSSIBLE TO MODIFY A PLANT GENOME AT A DESIRED SITE, WHICH IS UNLIKE CONVENTIONAL GENETIC ENGINEERING WHERE A TRANSGENE IS INTRODUCED AND INTEGRATES RANDOMLY INTO A GENOME. THERE HAS BEEN A SWIFT INCREASE IN APPLICATIONS OF CRISPR-CAS SYSTEMS DURING THE PAST FEW YEARS IN PART DUE TO THE LONG-TERM DESIRE FOR A TECHNOLOGY THAT ALLOWS PRECISE MODIFICATION AND IS STRAIGHTFORWARD TO DESIGN. ANOTHER ATTRACTIVE FEATURE IS THAT THE CRISPR-CAS TRANSGENE CAN BE GENETICALLY SEGREGATED AWAY, LEAVING ONLY THE TARGETED MODIFICATION IN THE PROGENY. BASED ON THE RECENTLY PASSED SECURE RULE, USDA-APHIS WILL NOT REGULATE GENE EDITED CROPS IF THE INTRODUCED DNA CHANGES CAN BE FOUND IN NATURE OR DERIVED FROM CONVENTIONAL BREEDING METHODS. SCIENCE BASED REGULATION WILL GREATLY ENCOURAGE THE USE OF GENE EDITING TECHNOLOGIES FOR FAST-TRACK CROP BREEDING TO MAKE IMPROVED CROPS THAT ARE NUTRITIOUS, HIGH YIELD, AND RESISTANT TO BIOTIC AND ABIOTIC STRESSES. GENE EDITING HAS ALSO GREATLY STIMULATED INNOVATION IN PLANT SYNTHETIC BIOLOGY AND AGRICULTURE. IN THIS PROJECT, WE PROPOSE TO ASSESS THREE EMERGING CRISPR GENOME EDITING SYSTEMS FOR ANY UNINTENDED MODIFICATIONS AT OTHER LOCI IN THE GENOMES OF RECOVERED EDITED RICE LINES. WE SPECIFICALLY ADDRESS THE BRAG PROGRAM AREA "5G", WHERE IT CALLS FOR COMPARISON OF THE TYPES AND FREQUENCIES OF NUCLEIC ACID CHANGES INTRODUCED INTO IMPORTANT CROP GENOMES, VIA GENETIC ENGINEERING TECHNIQUES VERSUS OTHER PLANT BREEDING TECHNIQUES. THEREFORE, WE WILL SPECIFICALLY ASSESS FOR UNINTENDED OFF-TARGET EFFECTS IN THE GENOMES OF EDITED LINES RECOVERED FROM 1) CAS12B EDITING, 2) CAS12A BASED MULTIPLEXED EDITING, AND 3) CAS9 BASED PRIME EDITING. IN PARALLEL, WE WILL DO A COMPARISON WITH CONTROL LINES RECOVERED THROUGH THE PLANT REGENERATION AND TRANSFORMATION PHASES BECAUSE OF UNINTENDED SOMACLONAL VARIATIONS. THE RELEVANCE OF THE PROPOSED PROJECT TO THE BRAG PROGRAM IS VERY SIGNIFICANT BECAUSE OUR GOALS ARE CLOSELY ALIGNED WITH THE MISSION, IN THAT THE RESULTS WILL PROVIDE INFORMATION ACCESSIBLE BY FEDERAL REGULATORY AGENCIES TO CONSIDER DURING EVALUATION FOR INTRODUCTION OF CRISPR-CAS GENERATED ORGANISMS INTO THE ENVIRONMENT. THE KNOWLEDGE GENERATED FROM OUR WORK WILL GREATLY AID PLANT SCIENTISTS PRACTICING GENE EDITING IN CROPS AS WELL AS FACILITATE THE DECISION-MAKING PROCESS AT REGULATORY AGENCIES SUCH AS USDA AND FDA.
$499,400University Of Maryland, College Park · · FY2020 · National Institute of Food and Agriculture
Hippo and Wnt signaling in cardiac regeneration
$499,306James Francis Martin · Texas Heart Institute · R01 · FY2018 · HL
Hippo and Wnt signaling in cardiac regeneration
$499,306James Francis Martin · Texas Heart Institute · R01 · FY2019 · HL
Advancing manufacturing and release testing of a gene editing therapy for Duchenne muscular dystrophy
$499,300Courtney S Young · Myogene Bio, Llc · SB1 · FY2025 · AR
Co-targeting the HER3 oncogenic signaling circuitry and PD-1 as a novel multimodal precision immunotherapy for HNSCC
$499,263J. Silvio Gutkind · University Of California, San Diego · R01 · FY2021 · CA
Characterizing patient-specific TBR1 mutations: Understanding a master regulator of autism risk.
$499,244Brian James O'Roak · Oregon Health & Science University · R01 · FY2018 · MH
Characterizing patient-specific TBR1 mutations: Understanding a master regulator of autism risk.
$499,244Brian James O'Roak · Oregon Health & Science University · R01 · FY2021 · MH
Characterizing patient-specific TBR1 mutations: Understanding a master regulator of autism risk.
$499,244Brian James O'Roak · Oregon Health & Science University · R01 · FY2019 · MH