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17,828 grants matching “crispr”
**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** TUTA ABSOLUTA (MEYRICK 1971), COMMONLY REFERRED TO AS TOMATO LEAF MINER, TOMATO BORER, OR THE SOUTH AMERICAN TOMATO PINWORM, IS ONE OF THE MOST DEVASTATING PESTS OF TOMATO (BAHAMONDES AND MALLEA 1969; POVOLNY 1994; ?URI? ET. AL 2014; BIONDI ET AL. 2018). FRESH AND PROCESSING TOMATOES REPRESENT A MASSIVE ECONOMIC INDUSTRY WORLDWIDE WITH AN ESTIMATED 241 MILLION METRIC TONS OF TOMATOES HARVESTED IN 2017 (FAOSTAT 2017). ALTERNATIVE HOSTS OF T. ABSOLUTA INCLUDE OTHER SOLANACEOUS PLANTS SUCH AS POTATOES AND EGGPLANTS, WHICH ALSO REPRESENT SIGNIFICANT AGRICULTURAL CROPS. NATIVE TO SOUTH AMERICA, T. ABSOLUTA WAS CONFINED TO THAT CONTINENT UNTIL IT WAS IDENTIFIED IN SPAIN IN 2006 (DESNEUX ET AL. 2010; GUILLEMAUD ET AL. 2015). IT HAS NOW SPREAD TO ALMOST EVERY CONTINENT, THREATENING COUNTRIES WHOSE ECONOMIES AND FOOD SECURITY RELY HEAVILY ON TOMATOES (CABI 2016; CAMPOS ET AL. 2017; MUTAMISWA ET AL. 2017; BIONDI ET AL. 2018; MANSOUR ET AL. 2018; HAN ET AL. 2018; 2019). THIS INSECT CAUSES DAMAGE TO ALL DEVELOPMENTAL STAGES OF ITS HOST PLANT, LEADING TO CROP LOSSES AS HIGH AS 80 TO 100% (BIONDI ET AL. 2018; GODFREY ET AL. 2018). ALTHOUGH T. ABSOLUTA HAS YET TO BE FOUND IN THE UNITED STATES (U.S.), WHICH MAKES UP A LARGE PORTION OF THE TOMATO PRODUCTION IN THE WORLD, COMPUTER MODELS PROJECT A HIGH LIKELIHOOD OF INVASION (USDA 2011; TONNANG ET AL. 2015; BIONDI ET AL. 2018). TO HALT THE CONTINUED SPREAD OF T. ABSOLUTA AND MINIMIZE ECONOMIC LOSS ASSOCIATED WITH THE TOMATO SUPPLY CHAIN AS WELL AS POTATO AND EGGPLANT PRODUCTION, ACCURATE AND EFFICIENT METHODS TO IDENTIFY T. ABSOLUTA AND STRENGTHEN SURVEILLANCE PROGRAMS ARE URGENTLY NEEDED. CURRENT IDENTIFICATION OF T. ABSOLUTA RELIES ON EXAMINATION OF MORPHOLOGY AND ASSESSMENT OF HOST PLANT DAMAGE, WHICH ARE DIFFICULT TO DIFFERENTIATE FROM THAT OF NATIVE GELECHIIDAE PESTS. TO ADDRESS THIS NEED, WE HAVE RECENTLY SEQUENCED THE GENOMES OF T. ABSOLUTA AND TWO CLOSELY RELATED GELECHIIDAE THAT ARE COMMONLY FOUND IN CALIFORNIA, THE TOMATO PINWORM KEIFERIA LYCOPERSICELLA (WALSHINGHAM 1897) AND THE POTATO TUBER MOTH PHTHORIMAEA OPERCULELLA (ZELLER 1873) (TABULOC ET AL. 2019). THE OVERALL GOAL OF THIS PROJECT IS TO LEVERAGE THESE NEW GENOMIC RESOURCES WE HAVE DEVELOPED TO DEVELOP RAPID MOLECULAR DIAGNOSTICS TO DIFFERENTIATE T. ABSOLUTA OF ALL LIFE STAGES FROM CLOSELY RELATED GELECHIIDS THAT ARE NATIVE TO THE U.S. SPECIFICALLY, WE WILL (1) SEQUENCE GENOMES OF GELECHIIDS THAT ARE MORPHOLOGICALLY SIMILAR TO T. ABSOLUTA, IN ADDITION TO SPECIES WE HAVE ALREADY SEQUENCED, (2) PERFORMED BIOINFORMATIC ANALYSIS TO DESIGN SPECIES DIAGNOSTICS, (3) VALIDATE TAQMAN AND CRISPR-BASED DIAGNOSTICS IN THE LABORATORY, AND (4) VALIDATE IN-FIELD CRISPR-BASED MOLECULAR DIAGNOSTICS FOR SPECIES IDENTIFICATION. MORE EFFICIENT AND ACCURATE DIAGNOSTICS WILL DECREASE TIME REQUIRED FOR POSITIVE IDENTIFICATION BY REGULATORS AND REDUCE LENGTH OF COSTLY QUARANTINE HOLDS FOR THE CROP INDUSTRIES. FURTHERMORE, DEVELOPMENT OF IN-FIELD DIAGNOSTICS WILL FACILITATE MONITORING EFFORTS BY CROP CONSULTANTS AND REGULATORY PERSONNEL, ENABLE PROMPT RESPONSE TO PREVENT THE ESTABLISHMENT OF T. ABSOLUTA, AND CONTAIN ITS SPREAD. THE PRIMARY BENEFICIARIES OF THIS PROJECT ARE INDUSTRIES INVOLVED IN THE PRODUCTION OF TOMATOES, POTATOES, AND EGGPLANTS, WHICH REPRESENT MAJOR HOSTS FOR T. ABSOLUTA. BEYOND THIS PROJECT, THE DIAGNOSTIC TECHNOLOGIES WE DEVELOP CAN BE BROADLY UTILIZED TO DETECT OTHER KEY INSECT PESTS OR PLANT DISEASE AGENTS.
$499,977University Of California, Davis · · FY2020 · National Institute of Food and Agriculture
**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** HAZARD COMPARISONS, UNDER FIELD CONDITIONS, OF GE-DERIVED, CRISPR-MUTANT, AND WILD-TYPE TOMATO LINES THAT VARY IN PLANT DEFENSE EXPRESSION
$499,967University Of Florida · · FY2022 · National Institute of Food and Agriculture
Innovative Approaches to Treat Duchenne Muscular Dystrophy Using iPSC-Derived Muscle Progenitors
$499,954Yao Liang Tang · Augusta University · R01 · FY2021 · AR
GENETIC MODIFICATION (GM) TECHNOLOGY SHOWS PROMISING RESULTS FOR INCREASING NUTRITIONAL QUALITY AND YIELD OF CROPS. MORE RECENTLY, GENOME EDITING (GE) TECHNOLOGY USING CRISPR (CLUSTERED REGULARLY INTERSPERSED SHORT PALINDROMIC REPEATS) ASSOCIATED CAS9/SGRNA SYSTEM SHOWS GREAT POTENTIAL FOR CROP IMPROVEMENT. HOWEVER, CONSUMERS HARBOR NEGATIVE VIEWS TOWARD GM AND GE FOOD PRODUCTS. CONSUMER CONCERNS INCLUDE THE POTENTIAL FOR GENOMIC VARIANTS AND UNPREDICTABLE PHENOTYPIC CONSEQUENCES DUE TO THE INSERTION OF A TRANSGENE OR OFF-TARGET MUTATIONS GENERATED BY CRISPR/CAS9. MODERN NON-TRANSGENIC PLANT BREEDING TECHNIQUES (E.G., IRRADIATION OR CHEMICAL MUTAGENESIS, TISSUE CULTURE, SOMATIC HYBRIDIZATION, AND WIDE CROSSING) ARE USED ON A WIDE SCALE, AND MORE THAN 2,000 CROP VARIETIES DERIVED FROM MODERN NON-TRANSGENIC PLANT BREEDING TECHNIQUES HAVE ENTERED THE FOOD SUPPLY WITHOUT SPECIFIC SAFETY REGULATIONS. HOWEVER, THESE TECHNIQUES ALSO CREATE A VARIETY OF PLANT GENOME MUTATIONS THAT ARE COMPARABLE TO THOSE CREATED BY TRANSGENIC AND GENE EDITING PLANT BREEDING, AND IT IS STILL QUESTIONABLE WHETHER THESE MUTATIONS ARE OF A SIMILAR MAGNITUDE AND TYPE TO THOSE INTRODUCED BY GM AND GE PLANT BREEDING METHODS.USING TOMATO, WHICH IS ONE OF THE HIGHEST PRODUCED EDIBLE CROPS IN THE WORLD AND IS AN EXCELLENT MODEL PLANT FOR THE STUDY OF COMPARATIVE GENOMICS AND PHENOMICS, THE GOAL OF THIS STUDY IS TO COMPARE AND CONTRAST THE TYPES AND FREQUENCIES OF GENOMIC CHANGES ASSOCIATED WITH UNINTENDED PHENOTYPIC VARIATIONS RESULTING FROM CRISPR/CAS9-MEDIATED GENOME-EDITED TOMATO VERSUS EMS-MUTAGENIZED TOMATO, AS WELL AS TRANSGENIC TOMATO VERSUS TISSUE CULTURE-DERIVED NON-TRANSGENIC TOMATO. THE SPECIFIC OBJECTIVES ARE PROPOSED: WE WILL 1) GENERATE CRISPR/CAS9-MEDIATED PURPLE-KNOCKOUT TOMATO LINES AND EMS-MUTAGENIZED PURPLE-LOSS TOMATO LINES; 2) PERFORM WHOLE GENOME SEQUENCING IN THE CRISPR/CAS9-MEDIATED PURPLE-KNOCKOUT LINES AND EMS-MUTAGENIZED PURPLE-LOSS LINES, ALONG WITH TRANSGENIC PURPLE-MONITORING TOMATO LINES EXPRESSING DEL/ROS1 TRANSGENE AND TISSUE CULTURE-DERIVED NON-TRANSGENIC LINES WHICH ARE NOT ASSOCIATED WITH INSERTION OF THE TRANSGENE; 3) PERFORM COMPARATIVE TRANSCRIPTOMIC PROFILING AND WHOLE-GENOME DNA METHYLATION PROFILING IN THE SET OF THOSE LINES; AND 4) INVESTIGATE PHENOTYPIC VARIATIONS AND POTENTIAL CONSEQUENCES IN THE LINES. THE RESULTS OF A WHOLE GENOME SEQUENCING, COMPARATIVE TRANSCRIPTOME PROFILING, DNA METHYLATION PROFILING, AND OTHER PHENOTYPING APPROACHES WILL PROVIDE FEDERAL REGULATORY AGENCIES WITH RELEVANT NEW GENOMIC AND UNINTENDED PHENOTYPIC INFORMATION OF TRANSGENIC AND GENE EDITING CROPS. THIS PROJECT ALIGNS WITH USDA BRAG PROGRAM AREA #5: OTHER RESEARCH TOPICS DESIGNED TO FURTHER THE PURPOSES OF THIS PROGRAM: COMPARISON OF THE TYPES AND FREQUENCIES OF NUCLEIC ACID CHANGES INTRODUCED INTO IMPORTANT CROP GENOMES, VIA GENETIC ENGINEERING TECHNIQUES VERSUS OTHER PLANT BREEDING TECHNIQUES.
$499,894Kansas State University · · FY2019 · National Institute of Food and Agriculture
Collaborative Research: EDGE FGT: Functional Genomic Tools for Parasitic Nematodes and their Bacterial Symbionts
$499,872Paul W Sternberg · California Institute Of Technology · · FY2021 · BIO
THE YIELD AND QUALITY OF FISH FILLETS ARE DETERMINED BY CELLULAR PROCESSES THAT GOVERN HOW MUSCLE TISSUE GROWS AND DEVELOPS. THE ACTIVIN/MYOSTATIN CELLULAR SIGNALING PATHWAY IS ONE OF THE MOST IMPORTANT MUSCLE GROWTH PATHWAYS IN VERTEBRATES AND WORKS BY DIRECTING MUSCLE FIBERS TO LIMIT THEIR OVERALL SIZE. DISRUPTION OF THE PATHWAY LEADS TO LARGE INCREASES IN MUSCLE MASS IN MAMMALS AND FISH. WHILE THE SIGNALING PROTEIN MYOSTATIN IS A WELL-ESTABLISHED ACTIVATOR OF THIS PATHWAY, IT DOES NOT ACT ALONE. IN MAMMALS, ACTIVIN A HAS AN EQUAL AND OVERLAPPING FUNCTION WITH MYOSTATIN TO SLOW MUSCLE GROWTH. OTHER THAN MYOSTATIN, THE FUNCTION OF THE BROADER ACTIVIN/MYOSTATIN SIGNALING PATHWAY IN FISH IS POORLY UNDERSTOOD. WE HYPOTHESIZE THAT ACTIVIN A HAS AN ESSENTIAL ROLE IN CONTROLLING THE SIZE OF MUSCLE TISSUE IN FISH AND THAT THERE ARE SPECIFIC MECHANISMS BY WHICH THE PATHWAY COORDINATES SIGNALING ACROSS MUSCLE AND NON-MUSCLE TISSUES. TO ADDRESS THESE HYPOTHESES, THE RESEARCH WILL FIRST ESTABLISH A COMPREHENSIVE ATLAS OF THE EXPRESSION OF ALL ACTIVIN/MYOSTATIN SIGNALING PATHWAY MEMBERS ACROSS 11 TISSUES AND 12 MUSCLE DEVELOPMENTAL STAGES, USING COMMERCIAL RAINBOW TROUT (RBT) AS A MODEL SYSTEM. CRISPR GENOME EDITING TECHNOLOGY WILL ALSO BE USED TO DISRUPT THE FUNCTION OF ALL FOUR ACTIVIN A GENES IN RBT AND INVESTIGATE WHETHER LOSS OF ACTIVIN A SIGNALING RESULTS IN AN INCREASE IN MUSCLE SIZE IN FISH. THESE GENE-EDITED LINES WILL BE PRODUCED SOLELY AS A BASIC RESEARCH MODEL SYSTEM AND ARE EXPECTED TO PROVIDE A WEALTH OF KNOWLEDGE ON THE PHYSIOLOGICAL FUNCTION OF ACTIVIN A IN FISH. BY DEVELOPING A DETAILED GENE EXPRESSION ATLAS FOR THE PATHWAY, THE RESEARCH WILL CREATE AN IMPORTANT RESOURCE TO THE SCIENTIFIC COMMUNITY, THE FIRST TO BE ESTABLISHED FOR ANY SPECIES TO DATE. THE IMPACT OF THE PROPOSED BASIC RESEARCH WILL FACILITATE A DEEPER UNDERSTANDING OF PERHAPS THE MOST IMPORTANT MUSCLE GROWTH PATHWAY IN FISH. THIS WILL PROVIDE THE FOUNDATION FOR EXPLOITING THE PATHWAY TO IMPROVE THE OVERALL YIELD AND QUALITY FISH FILLETS IN UNITED STATES FINFISH AQUACULTURE.
$499,859Washington State University · · FY2021 · National Institute of Food and Agriculture
Epigenetic and microenvironmental regulation of dormant disseminated cancer
$499,826Julio A Aguirre-Ghiso · Icahn School Of Medicine At Mount Sinai · R01 · FY2019 · CA
ADVANCING GENETICALLY ENGINEERED PLANTS FROM THE LAB TO THE FIELD AND EVEN THE EVENTUAL LARGE-SCALE PRODUCTION, CAN BE HAMPERED BY THE REGULATORY PROCESS BECAUSE OF THE COSTS INCURRED DURING THE LENGTHY PROCESS. THE NECESSARY REGULATORY COSTS, WHICH ALSO INCLUDE THOSE RELATED TO EXTENSIVE TESTING, MAKE IT LESS LIKELY THAT INNOVATIVE APPROACHES FOR CROP IMPROVEMENT DEVELOPED BY THE PUBLIC SECTOR AND SMALL BIOTECHNOLOGY COMPANIES WILL BE REALIZED. AS A RESULT, ANY NEW GENETICALLY MODIFIED (GM) CROPS ARE MORE LIKELY TO BE PRODUCED BY LARGE MULTINATIONAL COMPANIES, AND EVEN THEN, IT IS MAINLY LIMITED TO CROPS SUCH AS MAIZE AND SOYBEAN THAT HAVE LARGE GLOBAL MARKET VALUES. THE CRISPR (CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS)-CAS9 BASE EDITING SYSTEMS ARE POISED TO REVOLUTIONIZE MODERN CROP BREEDING DUE TO THEIR PROMISING APPLICATIONS FOR GENERATING TRANSGENE-FREE CROPS WITH QUANTITATIVE TRAITS INTRODUCED AT BASE PRECISION AT THE DNA LEVEL. BASE EDITING TECHNOLOGY PROVIDESAN EFFICIENT AND INEXPENSIVE WAY FORWARD FOR CROP BREEDING COMPARED TO CONVENTIONAL BREEDING METHODS. THIS PROJECT IS TO ADDRESS AN IMPORTANT KNOWLEDGE GAP, WHICH IS THE SAFETY OF PLANT BASE EDITING SYSTEMS. WE WILL FIRST IMPROVE THE EXISTING CRISPR-CAS9 C TO T AND A TO G BASE EDITING SYSTEMS FOR HIGHER EFFICIENCY AND ALTERED EDITING WINDOWS. NEXT, WE WILL GENERATE BASE-EDITED LINES OF TWO IMPORTANT FOOD CROPS, RICE AND TOMATO, AND USE WHOLE-GENOME SEQUENCING TO ASSESS UNINTENDED OFF-TARGET EFFECTS. IN PARALLEL, WE WILL ALSO SEQUENCE CONTROL LINES RECOVERED THROUGH THE PLANT REGENERATION AND TRANSFORMATION PHASES TO DETERMINE THE INCIDENCE OF UNINTENDED AFFECTS THAT CAN RESULT FROM SOMACLONAL VARIATION. THE RELEVANCE OF THE PROPOSED PROJECT TO THE BRAG PROGRAM IS VERY SIGNIFICANT BECAUSE OUR GOALS ARE CLOSELY ALIGNED WITH THE MISSION, IN THAT THE RESULTS WILL PROVIDE INFORMATION ACCESSIBLE BY FEDERAL REGULATORY AGENCIES TO CONSIDER DURING EVALUATION FOR INTRODUCTION OF CRISPR-CAS GENERATED ORGANISMS INTO THE ENVIRONMENT. THE KNOWLEDGE GENERATED FROM OUR WORK WILL GREATLY AID PLANT SCIENTISTS PRACTICING BASE EDITING TECHNOLOGY AS WELL AS FACILITATE THE DECISION-MAKING PROCESS AT REGULATORY AGENCIES SUCH AS USDA AND FDA. THIS RESEARCH PROJECT IS TO ADDRESS THE BRAG PROGRAM'S AREA 5G, WHERE IT CALLS FOR COMPARISON OF THE TYPES AND FREQUENCIES OF NUCLEIC ACID CHANGES INTRODUCED INTO IMPORTANT CROP GENOMES, VIA GENETIC ENGINEERING TECHNOLOGIES VERSUS OTHER PLANT BREEDING TECHNIQUES.
$499,607University Of Maryland, College Park · · FY2018 · National Institute of Food and Agriculture
CRISPR Microfluidic Array for Simultaneous Multiplexed Sensing of Proteins and miRNAs
$499,582James F Rusling · University Of Connecticut · · FY2023 · ENG
Mitochondrial Biogenesis in Health and Disease
$499,557Antoni Barrientos · University Of Miami School Of Medicine · R35 · FY2016 · GM
Systems Immunogenetics of Influenza Virus Infection in the Collaborative Cross
$499,496Mark T Heise · Univ Of North Carolina Chapel Hill · U19 · FY2019 · AI
Modeling KRAS-Dependent Synthetic Lethality in Human Colon Organoids
$499,437Calvin J Kuo · Stanford University · U01 · FY2019 · CA
WITH THE ADVANCES OF GENE EDITING TECHNOLOGIES, IT IS NOW POSSIBLE TO MODIFY A PLANT GENOME AT A DESIRED SITE, WHICH IS UNLIKE CONVENTIONAL GENETIC ENGINEERING WHERE A TRANSGENE IS INTRODUCED AND INTEGRATES RANDOMLY INTO A GENOME. THERE HAS BEEN A SWIFT INCREASE IN APPLICATIONS OF CRISPR-CAS SYSTEMS DURING THE PAST FEW YEARS IN PART DUE TO THE LONG-TERM DESIRE FOR A TECHNOLOGY THAT ALLOWS PRECISE MODIFICATION AND IS STRAIGHTFORWARD TO DESIGN. ANOTHER ATTRACTIVE FEATURE IS THAT THE CRISPR-CAS TRANSGENE CAN BE GENETICALLY SEGREGATED AWAY, LEAVING ONLY THE TARGETED MODIFICATION IN THE PROGENY. BASED ON THE RECENTLY PASSED SECURE RULE, USDA-APHIS WILL NOT REGULATE GENE EDITED CROPS IF THE INTRODUCED DNA CHANGES CAN BE FOUND IN NATURE OR DERIVED FROM CONVENTIONAL BREEDING METHODS. SCIENCE BASED REGULATION WILL GREATLY ENCOURAGE THE USE OF GENE EDITING TECHNOLOGIES FOR FAST-TRACK CROP BREEDING TO MAKE IMPROVED CROPS THAT ARE NUTRITIOUS, HIGH YIELD, AND RESISTANT TO BIOTIC AND ABIOTIC STRESSES. GENE EDITING HAS ALSO GREATLY STIMULATED INNOVATION IN PLANT SYNTHETIC BIOLOGY AND AGRICULTURE. IN THIS PROJECT, WE PROPOSE TO ASSESS THREE EMERGING CRISPR GENOME EDITING SYSTEMS FOR ANY UNINTENDED MODIFICATIONS AT OTHER LOCI IN THE GENOMES OF RECOVERED EDITED RICE LINES. WE SPECIFICALLY ADDRESS THE BRAG PROGRAM AREA "5G", WHERE IT CALLS FOR COMPARISON OF THE TYPES AND FREQUENCIES OF NUCLEIC ACID CHANGES INTRODUCED INTO IMPORTANT CROP GENOMES, VIA GENETIC ENGINEERING TECHNIQUES VERSUS OTHER PLANT BREEDING TECHNIQUES. THEREFORE, WE WILL SPECIFICALLY ASSESS FOR UNINTENDED OFF-TARGET EFFECTS IN THE GENOMES OF EDITED LINES RECOVERED FROM 1) CAS12B EDITING, 2) CAS12A BASED MULTIPLEXED EDITING, AND 3) CAS9 BASED PRIME EDITING. IN PARALLEL, WE WILL DO A COMPARISON WITH CONTROL LINES RECOVERED THROUGH THE PLANT REGENERATION AND TRANSFORMATION PHASES BECAUSE OF UNINTENDED SOMACLONAL VARIATIONS. THE RELEVANCE OF THE PROPOSED PROJECT TO THE BRAG PROGRAM IS VERY SIGNIFICANT BECAUSE OUR GOALS ARE CLOSELY ALIGNED WITH THE MISSION, IN THAT THE RESULTS WILL PROVIDE INFORMATION ACCESSIBLE BY FEDERAL REGULATORY AGENCIES TO CONSIDER DURING EVALUATION FOR INTRODUCTION OF CRISPR-CAS GENERATED ORGANISMS INTO THE ENVIRONMENT. THE KNOWLEDGE GENERATED FROM OUR WORK WILL GREATLY AID PLANT SCIENTISTS PRACTICING GENE EDITING IN CROPS AS WELL AS FACILITATE THE DECISION-MAKING PROCESS AT REGULATORY AGENCIES SUCH AS USDA AND FDA.
$499,400University Of Maryland, College Park · · FY2020 · National Institute of Food and Agriculture
Identification of metabolic regulators of glycerolipid synthesis and storage
$499,389Kivanc Birsoy · Rockefeller University · R01 · FY2021 · DK
HSET as a racial disparity biomarker for TNBC patients
$499,238Ritu Aneja · University Of Alabama At Birmingham · R01 · FY2024 · CA
Tumor-Targeted STING Agonism in Head and Neck Squamous Cell Carcinoma
$499,238Thomas Hayman · Yale University · R37 · FY2025 · CA
Pre-clinical development of SPY-DYS45-55, a CRISPR/Cas9 platform for Duchenne muscular dystrophy
$499,207Courtney S Young · Myogene Bio, Llc · R44 · FY2022 · AR
Mechanisms of Control of Lymphocyte Activation and Proliferation by a Critical Signaling Integrator
$499,154Joel L Pomerantz · Johns Hopkins University · R01 · FY2023 · AI
Identifying the origins of resilience through human single cell molecular networks, then testing them in diverse, resilient, human IPS lines
$499,005Christopher A. Gaiteri · Rush University Medical Center · R01 · FY2022 · AG
California National Primate Research Center Development of a Nonhuman Primate Model of Long COVID
$498,994Prasant Mohapatra · University Of California At Davis · P51 · FY2021 · OD
THIS RESEARCH ADDRESSES AREA 5A OF THE USDA BIOTECHNOLOGY RISK ASSESSMENT CALL FOR PROPOSALS, INCLUDING RESEARCH ADDRESSING THE UNINTENDED EFFECTS IN BIOENGINEERED ORGANISMS DEVELOPED USING GENOME EDITING TECHNOLOGY OR OTHER ENGINEERING TECHNIQUES.ADVANCES IN BIOLOGY AND GENE EDITING METHODS ARE ENABLING THE ADVANCED MODIFICATIONS OF GENOMES IN A WIDE VARIETY OF CROP SPECIES. GENES THAT CONTROL SHOOT DEVELOPMENT ARE POWERFUL TOOLS FOR HELPING GENE TRANSFER AND EDITING IN MANY DIFFICULT CROP VARIETIES, BUT USUALLY MUST BE REMOVED (EXCISED) FROM THE GENOME TO ENABLE NORMAL PLANT REGENERATION AND FERTILITY. IN VEGETATIVELY PROPAGATED CROPS, GENE EDITING AGENTS SUCH AS CRISPR CANNOT BE REMOVED DURING SEXUAL REPRODUCTION AS IS COMMON FOR ANNUAL CROPS, THUS ALSO BENEFIT FROM EXCISION METHODS. THE MOST COMMON TOOL FOR EXCISION IS THE CRE RECOMBINASE, HOWEVER, CRE EXPRESSION MAY IMPART UNDESIRABLE AND SUBSTANTIAL UNINTENDED GENOME MODIFICATIONS.USING POPLAR AS A TEST DICOT CROP, WE PROPOSE TO: 1) COMPLETE THE GENERATION AND TESTING OF A SYSTEM FOR CONTROLLED EXPRESSION OF CRE FOR TRANSGENE AND CRISPR-CAS REMOVAL IN CLONALLY PROPAGATED DICOT CROPS; 2) OPTIMIZE THE SYSTEM WITH RESPECT TO ITS CAPACITY TO PRODUCE AN EFFICIENT RATE OF EDITING AND EXCISION WITH MINIMAL CHIMERISM; 3) PRODUCE PROTOTYPE EDITED AND EXCISED REGENERANTS, AND ANALYZE EFFECTS OF CRE ACTIVITY ON VARIATION IN PLANT BIOMASS PRODUCTIVITY IN THE GREENHOUSE, GENOME-SCALE CHROMOSOME AND DNA BASE PAIR VARIATION, AND NEAR TO THE INSERTION/EXCISION SITE IMPACTS ON GENE EXPRESSION AND CHROMOSOME STRUCTURE. THIS WORK WILL HELP TO INFORM REGULATORS OF THE DEGREE TO WHICH THEY SHOULD BE CONCERNED WITH OFF-TARGET IMPACTS WHEN CRE RECOMBINASES ARE USED FOR GENOME EDITING.
$498,991Oregon State University · · FY2020 · National Institute of Food and Agriculture
Androgen Activation of Innate Immune Signaling to Enhance Prostate Cancer Immune Response
$498,873Sushant Krishna Kachhap · Johns Hopkins University · R01 · FY2024 · CA
Epigenetic mechanisms of transcriptional activation of a novel oncogenic ALK variant in cancer
$498,691Ping Chi · Sloan-Kettering Inst Can Research · R01 · FY2021 · CA
Epigenetic mechanisms of transcriptional activation of a novel oncogenic ALK variant in cancer
$498,691Ping Chi · Sloan-Kettering Inst Can Research · R01 · FY2020 · CA
Epigenetics of inner ear neurosensory cell development
$498,669Donna M Martin · University Of Michigan At Ann Arbor · R01 · FY2021 · DC