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17,828 grants matching “crispr”
Combine Genomics and Symptoms Data Driven Models to Discover Synergistic Combinatory Therapies for Alzheimer's Disease
$500,322Fuhai Li · Washington University · R56 · FY2020 · AG
Targeting ATM to boost systemic effects of radiotherapy and immunotherapy
$500,236Yunyan Jennifer Jennifer Zhang · Duke University · R01 · FY2024 · CA
Control of PGC1alpha Translation and Function
$500,197Bruce M Spiegelman · Dana-Farber Cancer Inst · R01 · FY2019 · DK
Project 2: Impact of H1/H2 haplotypes on cellular disease-associated phenotypes driven by FTD-causing MAPT mutations
$500,156Daniel H Geschwind · Icahn School Of Medicine At Mount Sinai · U54 · FY2021 · NS
The role of PHF6 in the control of hematopoietic stem cell aging.
$500,000Adolfo A. Ferrando · Columbia University Health Sciences · R01 · FY2021 · AG
FMSG: Bio: Integrated bioprocess and synthetic biology for future biomanufacturing of industrial products
$500,000Shang-Tian Yang · Ohio State University, The · · FY2023 · BIO
The role of PHF6 in the control of hematopoietic stem cell aging.
$500,000Teresa Palomero · Columbia University Health Sciences · R01 · FY2024 · AG
DUE TO EASE OF USE, CRISPR-CAS9 HAS ADVANCED REMARKABLY IN THE PAST FEW YEARS AND HAS BECOME A UBIQUITOUS GENOME-EDITING METHOD BEING APPLIED TO AN INCREASING NUMBER OF SPECIES. WHILE SEVERAL SUCCESSFUL CASES OF CROP IMPROVEMENT USING VARIOUS GENOME- EDITING PLATFORMS INCLUDING CRISPR SYSTEMS AND TALENS HAVE BEEN REPORTED, CONCERNS OF GENOME WIDE OFF-TARGETS HAVE NOT BEEN THOROUGHLY ADDRESSED. TREMENDOUS PROGRESS HAS BEEN MADE IN INCREASING THE SPECIFICITY AND THUS REDUCING OFF-TARGETING BY ENGINEERING CAS9 VARIANTS SUCH AS THE HYPER-ACCURATE CAS9 VARIANT, AN ALTERNATIVE CRISPR-CPF1 SYSTEM ANALOGOUS TO CRISPR-CAS9, AND A NEW BASE EDITING APPROACH, WHICH DOESN'T REQUIRE DNA CLEAVAGE TO INCORPORATE SPECIFIC MUTATIONS. REGULATORY AGENCIES NEED TO EVALUATE THE PRECISION OF THESE NEW AND EMERGING GENE- EDITING REAGENTS. WE PROPOSE TO USE THE CURRENT AND EMERGING GENOME-EDITING REAGENTS FOR TARGETED MUTAGENESIS OF COMMERCIALLY IMPORTANT CANDIDATE GENES IN TWO DIFFERENT CROP SPECIES, POTATO AND CAMELINA, WHICH HAVE CONTRASTING GENOMIC ARCHITECTURE AND MODES OF REPRODUCTION. IN POTATOMECHANICALDAMAGE TOTUBERS DURING HARVEST AND POST-HARVEST PROCEDURES LEAD TO DISCOLORATION DUE TO ENZYMATIC BROWNING CAUSED BY THE CANDIDATE GENEPOTATO POLYPHENOL OXIDASE (PPO). PPO CATALYZES THE CONVERSION OF O-DIHYDROXYPHENOLS TO O-QUINONES. THE QUINONES POLYMERIZE AND REACT WITH AMINO ACIDS RESULTING IN THE FORMATION OF BLACK/BROWN PIGMENTATION. OUR SECOND CANDIDATE GENE, DELTA-12-DESATURASE (FAD2) CONVERTS OLEIC ACID (18:1) FATTY ACID TO 18:2. BY KNOCKING OUTFAD2 WE RETAIN HIGH OLEIC ACID WHICH IS DESIRABLE IN INDUSTRIAL OILS.WE WILL COMPARE THE GENOME WIDE OFF-TARGET EFFECTS CAUSED BY THESE REAGENTS USING WHOLE GENOME SEQUENCING AND DETERMINE THE TYPE AND FREQUENCY OF ON- AND OFF-TARGET VARIATION ATTRIBUTABLE BY GENOME-EDITING REGEANTS. THIS STUDY WOULD PROVIDE A COMPREHENSIVE RISK ASSESSMENT OF GENOME- EDITED CROPS WITH CURRENT TECHNOLOGIES. THIS PROPOSAL ADDRESSES THE BRAG FOCUS AREA OF COMPARISON OF THE TYPES AND FREQUENCIES OF NUCLEIC ACID CHANGES INTRODUCED INTO IMPORTANT CROP GENOMES, VIA GENETIC INSERTION/MODIFICATION TECHNIQUES VERSUS OTHER PLANT BREEDING TECHNIQUES - OFF TARGET EFFECTS OF GENOME-EDITING TECHNOLOGIES.
$500,000Michigan State University · · FY2018 · National Institute of Food and Agriculture
GENETIC CONTAINMENT IN LIVESTOCK VIA CRISPR-MEDIATED GENE KNOCK-IN
$500,000University Of California, Davis · · FY2015 · National Institute of Food and Agriculture
**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** THE US CARROT CROP HAS AN ANNUAL VALUE OF $863 MILLION. CARROTS ARE THE 7TH MOST CONSUMED FRESH VEGETABLE IN THE US, AND THE US IS THE 4TH LARGEST PRODUCER OF CARROTS IN THE WORLD. CARROT IS WIDELY RECOGNIZED FOR ITS CONTRIBUTIONS TO HUMAN HEALTH THROUGH THE HIGH CONTENT OF PROVITAMIN A Β-CAROTENE IN ITS ROOT. THE PRODUCTION OF SUNFLOWERS FOR BOTH OIL AND NON-OIL PURPOSES HAS AN ANNUAL VALUE OF >$350 MILLION. SUNFLOWER IS ALSO THE #4 OIL CROP PRODUCED GLOBALLY. THE SUSTAINED COMMERCIAL INTEREST IN SUNFLOWER IS BASED IN LARGE PART BECAUSE OF THE INTRODUCTION OF HIGH- AND MID-OLEIC ACID OIL GERMPLASM THAT IS HIGHLY DESIRED FOR THE MANUFACTURE OF TRANS FAT-FREE BAKED AND FRIED FOODS.PLANTS ARE MASTER MANUFACTURERS OF USEFUL CHEMICALS, OR NATURAL PRODUCTS, THAT THEY NORMALLY USE TO PROTECT THEMSELVES AGAINST HERBIVORES AND MICROBIAL PATHOGENS, OR TO ATTRACT POLLINATORS. NATURAL PRODUCTS ACCOUNT FOR ONE-THIRD OF ALL FOOD AND DRUG ADMINISTRATION-APPROVED NOVEL DRUGS OR NEW MOLECULAR ENTITIES (NMES); 25% OF THESE ARE DERIVED FROM PLANTS. SIMILARLY, 11% OF THE 252 DRUGS CONSIDERED ESSENTIAL BY THE WORLD HEALTH ORGANIZATION ORIGINATED FROM FLOWERING PLANTS. DESPITE THEIR PREVALENCE AS THE SOURCE OF NUMEROUS PHARMACEUTICALS, THE ENTIRE SUITE OF BIOCHEMICAL MACHINERY NECESSARY FOR THE SYNTHESIS OF RELATIVELY FEW NATURAL PRODUCTS HAS BEEN IDENTIFIED.ONE IMPORTANT CLASS OF PLANT-DERIVED NATURAL PRODUCTS IS THE FALCARINS. FALCARINS ARE KNOWN TO ORIGINATE FROM LIPIDS FOUND IN ESSENTIALLY ALL LIVING ORGANISMS BUT THEIR SYNTHESIS IS RESTRICTED TO A HANDFUL OF PLANT FAMILIES WHICH INCLUDE FRESH-MARKET AND PROCESSING CROPS SUCH CARROTS AND OILSEEDS LIKE SUNFLOWER. FALCARINS ARE KNOWN FOR THEIR HEALTH PROMOTING, ANTI-CANCER AND ANTIMICROBIAL ACTIVITIES AND ARE THOUGHT TO BE CRITICAL FOR PLANT DEFENSE AGAINST AGRONOMICALLY-DEVASTATING PATHOGENS. ADDITIONALLY, SPECIFIC FALCARINS ARE KNOWN TO BE A COMPONENT OF THE BITTER TASTE OF CARROTS. DESPITE KNOWLEDGE OF THEIR EXISTENCE IN PLANTS FOR MORE THAN 50 YEARS, ONLY MODERATE PROGRESS HAS BEEN MADE ON UNDERSTANDING HOW PLANTS MAKE FALCARINS, WHICH IN TURN HAS RESULTED IN LITTLE IF ANY EMPHASIS ON THE USE OF THESE NATURAL PRODUCTS IN CROP IMPROVEMENT EFFORTS.THE GOAL OF THE PROPOSED RESEARCH IS TO FULLY IDENTIFY AND CHARACTERIZE THE BIOCHEMICAL MACHINERY THAT PLANTS USE TO MAKE FALCARINS USING CARROT AND SUNFLOWER AS AGRONOMICALLY-RELEVANT PLANT SPECIES. TO ACCOMPLISH THIS, CUTTING-EDGE GENOMICS AND GENOME EDITING TECHNIQUES, SUCH AS CRISPR, WILL BE EMPLOYED. THROUGH THIS WORK, CARROT GERMPLASM WITH ALTERED FALCARIN CONTENT AND COMPOSITION WILL BE GENERATED. THE GERMPLASM AND INFORMATION GENERATED THROUGH THIS RESEARCH WILL PROVIDE A VALUABLE RESOURCE FOR PLANT BREEDING EFFORTS AIMED AT IMPROVING CROP RESILIENCE, ENHANCING FLAVOR PROFILES, AND IMPROVING THE NUTRITIONAL QUALITY OF CARROTS THAT WILL TRANSLATABLE TO OTHER CROP SPECIES. THIS RESEARCH FURTHER STANDS TO ELEVATE THE IMPORTANCE OF FALCARINS FOR HUMAN HEALTH,AS ANTI-CANCER COMPONENTS THAT PROVIDE ADDED VALUE AND BENEFITS TO CARROT AND SUNFLOWER PRODUCTION AND CONSUMPTION. GIVEN THE DEMONSTRATED HEALTH-PROMOTING PROPERTIES OF FALCARINS, WE ENVISION THAT THIS RESEARCH WILL ADVANCE THE USE OF CARROT IN FUNCTIONAL FOOD APPLICATIONS, A GROWING SEGMENT OF THE FOOD MARKET.
$500,000Board Of Regents Of Nevada System Of Higher Education · · FY2021 · National Institute of Food and Agriculture
Comprehensive functional characterization and dissection of noncoding regulatory elements and human genetic variation
$500,000Pardis Christine Sabeti · Broad Institute, Inc. · UM1 · FY2018 · HG
The role of PHF6 in the control of hematopoietic stem cell aging.
$500,000Teresa Palomero · Columbia University Health Sciences · R01 · FY2023 · AG
Center for Functional Validation and Evaluation of ENCODE Enhancer Regions
$500,000Barbara E Stranger · University Of Chicago · UM1 · FY2018 · HG
FMSG: Bio: Advancing Extracellular Vesicle Biomanufacturing of CRISPR-Edited Human iPSC-derived MSCs with Next-Generation Purification
$500,000Zhen Ma · Syracuse University · · FY2023 · BIO
FMSG: Bio: End-to-End Continuous Manufacture of Cell Therapies Enabled by Robotics and Microfluidic Processing
$500,000Todd Sulchek · Georgia Tech Research Corporation · · FY2021 · BIO
Collaborative Research: Synthetic CRISPR-Cas6 endonucleases for dynamic control of cellular phenotypes in yeast
$500,000Wilfred Chen · University Of Delaware · · FY2020 · BIO
Collaborative Research: Synthetic CRISPR-Cas6 endonucleases for dynamic control of cellular phenotypes in yeast
$500,000Nancy A Da Silva · University Of California-Irvine · · FY2020 · BIO
CAREER: Enhancing Stem Cell Differentiation with CRISPR Activators via Non-integrating Methods
$500,000Xiaojun Lian · Pennsylvania State Univ University Park · · FY2020 · ENG
CAREER: Smartphone-Based CRISPR Biosensor for Point-of-Care HIV Viral Load Testing
$500,000Qingshan Wei · North Carolina State University · · FY2020 · ENG
A Bio-orthogonal Approach for Identifying Direct Kinase Substrates and their phosphorylation sites
$500,000Kavita Shah · Purdue University · · FY2017 · MPS
The role of PHF6 in the control of hematopoietic stem cell aging.
$500,000Teresa Palomero · Columbia University Health Sciences · R01 · FY2022 · AG
Equipment and Administrative Supplement- THE HARC CENTER 2.0: HIV ACCESSORY AND REGULATORY COMPLEXES
$500,000Nevan J Krogan · University Of California, San Francisco · U54 · FY2024 · AI
Development of a nanoparticle-based gene editing technology for neurological applications
$499,996Krystof S Bankiewicz · Ohio State University · UH3 · FY2022 · NS
Systematic identification of oncogenic KRAS synthetic lethal interactions
$499,996William C Hahn · Broad Institute, Inc. · U01 · FY2019 · CA
Defining cellular states of quiescence in human brain tumors
$499,981Patrick Paddison · Fred Hutchinson Cancer Center · R01 · FY2024 · NS