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17,054 grants matching “genome editing”
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** GROWING POPULATION AND SHRINKING AGRICULTURAL RESOURCES ARE ONE OF THE MOST IMPORTANT CHALLENGES FACING FOOD PRODUCTION. IN ORDER TO MEET THE PROJECTED INCREASE OF GLOBAL DEMAND FOR FOOD, FEED, AND FIBER (100% BY 2050), THE LINEAR PROGRESS WILL NEED TO BE INCREASED BY ACCELERATING THE EFFICIENCY, RELIABILITY, AND SPEED OF GENETIC IMPROVEMENT. DOUBLED HAPLOID (DH) TECHNOLOGY CAN SPEED UP INBRED LINE DEVELOPMENT BY REDUCING THE NUMBER OF GENERATIONS FROM SEVEN IN TRADITIONAL BREEDING TO TWO GENERATIONS IN DH BREEDING. THERE ARE TWO MAIN STEPS IN DH TECHNOLOGY. THE FIRST STEP IN A DH BREEDING PROGRAM IS TO PRODUCE HAPLOIDS FOR A DONOR GENOTYPE BY POLLINATING THE DONOR USING A MATERNAL HAPLOID INDUCER AS MALE. THE SECOND STEP IS TO PRODUCE DHS BY SELF-POLLINATING THE HAPLOIDS. HAPLOID MAIZE HAS A DECENT AMOUNT OF HAPLOID FEMALE FERTILITY, BUT LACK OF HAPLOID MALE FERTILITY (HMF) IS THE BOTTLENECK TO DEVELOP DH LINES EFFICIENTLY. TO OVERCOME THE HMF LIMITATION, HAPLOID SEEDLINGS ARE CHEMICALLY TREATED WITH COLCHICINE TO MEDIATE GENOME DOUBLING, WHICH IS LABOR- AND RESOURCE- INTENSIVE AND IT IS AN INEFFICIENT METHOD. THE ALTERNATIVE IS TO FIND GENETIC MECHANISM(S) TO RESTORE HMF.OUR PREVIOUS RESEARCH IN ARABIDOPSIS INFORMED US THAT MUTATIONS IN PARALLEL SPINDLE (PS) GENES ARE SUFFICIENT TO RESTORE HMF IN ARABIDOPSISAND THE DHS OBTAINED USING PS MUTATIONS HAVE NO CHROMOSOMAL ABNORMALITIES. THE GOAL OF THIS RESEARCH IS TO IDENTIFY MAIZE PS GENES WITH SEQUENCE SIMILARITY TO ARABIDOPSIS PS GENES. MUTATIONS BY TRANSPOSON INSERTION IN MAIZE PS GENES WILL BE IDENTIFIED FROM PUBLIC COLLECTION AND NEW MUTATION WILL BE CREATED BY GENOME EDITING EXPERIMENTS. HAPLOIDS WILL BE PRODUCED FOR THESE MUTANT PLANTS AND THEIR MALE FERTILITY WILL BE EVALUATED BY SCORING FOR THE PRESENCE OF ANTHERS AND POLLEN. ULTIMATELY, THIS PROJECT WILL IDENTIFY GENETIC MECHANISM(S) TO RESTORE HMF WITHOUT THE USE OF LABOR- AND RESOURCE- INTENSIVE AND TOXIC CHEMICAL METHODS. IN OTHER WORDS, HAPLOID SEEDS CAN BE DIRECTLY SOWN IN THE FIELD. GENETIC MECHANISM(S) TO RESTORE HMF HAS THE POTENTIAL TO MAKE DH TECHNOLOGY IN MAIZE BROADLY AVAILABLE, WHICH IS STILL MOSTLY CONFINED TO MAJOR CROP BREEDING PROGRAMS. FURTHER, THESE GENES ARE CONSERVED IN OTHER PLANT SPECIES OPENING THE POSSIBILITY FOR THE APPLICATION OF THIS TECHNOLOGY TO OTHER CROPS WHERE IT IS NOT AVAILABLE SO FAR.
$649,443Iowa State University Of Science And Technology · · FY2023 · National Institute of Food and Agriculture
Progenitor Regulation Underlying Cortical Interneuron Specification
$649,412Margaret Elizabeth Ross · Weill Medical Coll Of Cornell Univ · R01 · FY2021 · NS
Progenitor Regulation Underlying Cortical Interneuron Specification
$649,412Margaret Elizabeth Ross · Weill Medical Coll Of Cornell Univ · R01 · FY2020 · NS
MHC variation at high resolution in multiple sclerosis
$649,361Jorge R. Oksenberg · University Of California, San Francisco · R01 · FY2018 · NS
The Spatiotemporal Landscape of the Human Brain Epitranscriptome
$649,352Christopher Edward Mason · Weill Medical Coll Of Cornell Univ · R01 · FY2022 · MH
Genomics and functional dissection of fetal brain abnormalities using a prenatal cohort
$649,179Neeta L Vora · Univ Of North Carolina Chapel Hill · R01 · FY2023 · HD
Functional Characterization of Glioma GWAS Variants
$649,042Rose Kamyee Lai · University Of Southern California · R01 · FY2017 · CA
Functional Characterization of Glioma GWAS Variants
$649,042Rose Kamyee Lai · University Of Southern California · R01 · FY2018 · CA
MG53 function in muscle aging
$649,040Jianjie Ma · University Of Virginia · R01 · FY2022 · AG
Defining the complex genetic basis of Plasmodium falciparum resistance to artemisinin and quinine and identifying resistance-refractory therapeutics
$648,982David Armand Fidock · Columbia University Health Sciences · R01 · FY2025 · AI
University of Michigan Skin Biology and Diseases Resource-based Center
$648,650Johann Eli Gudjonsson · University Of Michigan At Ann Arbor · P30 · FY2025 · AR
Roles for uniquely human enhancers in brain development and WNT signaling
$648,639Debra Silver · Duke University · R01 · FY2025 · MH
A scalable platform for target validation in GEMM models of gastrointestinal malignancies.
$648,617Scott W Lowe · Sloan-Kettering Inst Can Research · R01 · FY2016 · CA
Defining the complex genetic basis of Plasmodium falciparum resistance to artemisinin and quinine and identifying resistance-refractory therapeutics
$648,448David Armand Fidock · Columbia University Health Sciences · R01 · FY2024 · AI
Developing gene editing platforms for retinal degeneration.
$648,444Gaurav Sahay · Oregon State University · R01 · FY2022 · EY
Characterization of novel insulin resistance genes by gene editing, high-throughput phenotyping and in vivo studies
$648,352Joshua Wiley Knowles · Stanford University · R01 · FY2020 · DK
Molecular pathogenesis of blastomycosis
$648,248Bruce Steven Klein · University Of Wisconsin-Madison · R37 · FY2022 · AI
Define the oncogenic role of METTL3 in the pathogenesis of chronic lymphocytic leukemia
$648,241Lili Wang · Beckman Research Institute/City Of Hope · R01 · FY2025 · CA
Binding of Epstein Barr Virus EBNA2 Unifies Multiple Sclerosis Genetic Mechanisms
$648,175Matthew Tyson Weirauch · Cincinnati Childrens Hosp Med Ctr · R01 · FY2023 · NS
Defining the targets of broad intervention antimalarial agents
$648,140Elizabeth A Winzeler · University Of California, San Diego · R01 · FY2013 · AI
MHC variation at high resolution in multiple sclerosis
$648,105Jorge R. Oksenberg · University Of California, San Francisco · R01 · FY2019 · NS
Impact of mutational order on molecular mechanisms of oncogenesis
$648,012Eirini Papapetrou · Icahn School Of Medicine At Mount Sinai · R01 · FY2025 · CA
Structural Basis of APOBEC Functions and HIV Restriction
$647,853Xiaojiang S Chen · University Of Southern California · R01 · FY2025 · AI
RESEARCH PROJECT 1
$647,851Nevan J Krogan · University Of California, San Francisco · U19 · FY2020 · AI
in vivo modeling of IgH-reprogrammed B cells in mice
$647,573James Even Voss · Scripps Research Institute, The · P01 · FY2025 · HL