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17,828 grants matching “crispr”
JHU Center for Neuroscience Research
$652,000Alex L Kolodkin · Johns Hopkins University · P30 · FY2016 · NS
CRISPR Genome-Wide sgRNA Library Screening Platform
$651,831Donato Tedesco · Cellecta, Inc. · R44 · FY2017 · HG
Systematic Identification and Phenotypic Characterization of causal genetic variants in Rare Disease-Associated Birth Defects
$651,777Scott T Younger · Children'S Mercy Hosp (Kansas City, Mo) · R01 · FY2024 · HD
The role of retrotransposons in female reproductive aging
$651,426Lin He · University Of California Berkeley · R01 · FY2022 · AG
Engineering CAR-B cells for an HIV-1 functional cure
$651,390Michael R. Farzan · Boston Children'S Hospital · R01 · FY2024 · DA
Stoichiometric, adhesion, and contractile deficits in desmoplakin cardiomyopathy
$651,349Adam S Helms · University Of Michigan At Ann Arbor · R01 · FY2025 · HL
MELT: Modulation of PSMA Expression for Lutetium Therapy
$651,214Thomas Hope · University Of California, San Francisco · R01 · FY2021 · CA
Multi-organism platform for functional assessment of human birth defect associated genomic variants
$651,149Lilianna Solnica-Krezel · Washington University · R01 · FY2023 · HD
Characterization and targeting of the epigenetic state underlying uveal melanoma liver metastasis
$651,147Keiran Smalley · H. Lee Moffitt Cancer Ctr & Res Inst · R01 · FY2025 · CA
PROJECT 2: Determine clinically relevant host-viral dependency networks for respiratory infections including SARS-CoV-2
$651,020Melanie Maria Ott · University Of California, San Francisco · U19 · FY2025 · AI
(PQ#3) Novel tumor intrinsic PD-L1 signals direct tumor immune cell infiltration
$650,815Tyler J. Curiel · University Of Texas Hlth Science Center · R01 · FY2017 · CA
Mechanism of epigenetic activation during disease progression
$650,607David Takeda · Division Of Basic Sciences - Nci · ZIA · FY2021 · CA
Identification of epigenetic drivers of advanced prostate cancer
$650,607David Takeda · Division Of Basic Sciences - Nci · ZIA · FY2021 · CA
Lrp4 signaling in bone metabolism and mechanotransduction
$650,545Alexander G Robling · Indiana University Indianapolis · R01 · FY2017 · AR
Project 2-Identifying and exploiting metabolic vulnerabilities in PDAC
$650,399Nabeel Bardeesy · University Of Tx Md Anderson Can Ctr · P01 · FY2024 · CA
CAREER: Mechanistic Investigations of Conformational Activation and Catalysis in Emerging CRISPR-Cas Systems
$650,111Giulia Palermo · University Of California-Riverside · · FY2022 · MPS
CRISPR screens of population relevant genes governing toxicant resilience
$650,042Christopher D Vulpe · University Of Florida · R01 · FY2022 · ES
SALMONELLA REMAINS A PERSISTENT FOOD SAFETY CHALLENGE, ESPECIALLY IN POULTRY PRODUCTS LIKE GROUND TURKEY, WHICH ARE A SIGNIFICANT SOURCE OF HUMAN ILLNESS IN THE UNITED STATES. DESPITE MANY EFFORTS TO REDUCE CONTAMINATION, SALMONELLA CONTINUES TO CAUSE OUTBREAKS, HIGHLIGHTING THE NEED FOR A BETTER UNDERSTANDING OF HOW AND WHERE IT APPEARS DURING TURKEY PRODUCTION AND PROCESSING. TRADITIONALLY, FOOD SAFETY CONTROLS HAVE FOCUSED ON GENERAL PRACTICES TO REDUCE CONTAMINATION ACROSS THE BOARD. HOWEVER, RECENT EVIDENCE SUGGESTS THAT THESE EFFORTS MAY OVERLOOK KEY DIFFERENCES BETWEEN HOW SALMONELLA SURVIVES ON THE SURFACE (EXTERNAL CONTAMINATION) VERSUS INSIDE THE BIRD (INTERNAL CONTAMINATION). THIS PROJECT AIMS TO ADDRESS THIS CRITICAL GAP IN UNDERSTANDING BY INVESTIGATING SALMONELLA CONTAMINATION AT THE LEVEL OF INDIVIDUAL TURKEY CARCASSES AS THEY MOVE THROUGH PROCESSING.WHILE REGULATORY AGENCIES LIKE THE USDA'S FOOD SAFETY AND INSPECTION SERVICE (FSIS) HAVE MADE PROGRESS IN REDUCING SALMONELLA LEVELS IN POULTRY, CURRENT PUBLIC DATA AND SCIENTIFIC STUDIES ARE LIMITED, PARTICULARLY FOR TURKEYS, WHICH DIFFER FROM MORE COMMONLY STUDIED BROILERS. AS REGULATORY EXPECTATIONS INCREASE, THE TURKEY INDUSTRY LACKS DETAILED, SCIENCE-BASED INFORMATION NEEDED TO GUIDE BETTER CONTROL STRATEGIES, PRODUCT TESTING, AND FOOD SAFETY DECISIONS. THERE IS ALSO GROWING INTEREST IN UNDERSTANDING MICROBIAL INDEPENDENCE, OR HOW CONTAMINATION IN ONE BIRD MAY (OR MAY NOT) RELATE TO CONTAMINATION IN ANOTHER DURING PROCESSING. THIS MATTERS BECAUSE SUCH INFORMATION CAN HELP PRODUCERS DEFINE LOTS OF PRODUCT MORE ACCURATELY AND USE RISK-BASED SAMPLING METHODS THAT ARE BOTH EFFECTIVE AND PRACTICAL.TO FILL THESE CRITICAL KNOWLEDGE GAPS, THIS RESEARCH FOLLOWS A DETAILED PLAN WITH THREE MAJOR AIMS. THE FIRST AIM IS TO DETERMINE HOW MUCH SALMONELLA CONTAMINATION VARIES FROM ONE TURKEY CARCASS TO ANOTHER DURING PROCESSING, AND TO DISTINGUISH BETWEEN EXTERNAL AND INTERNAL CONTAMINATION. RESEARCHERS WILL COLLECT SAMPLES FROM THE SAME BIRDS AT MULTIPLE PROCESSING POINTS, FROM EARLY STAGES LIKE PRE-SCALD TO FINAL PRODUCTS LIKE GROUND TURKEY. THEY WILL USE ADVANCED TOOLS, INCLUDING RAPID DETECTION METHODS, MICROBIAL COUNTS, AND DEEP GENETIC TYPING (CRISPR-SEROSEQ), TO TRACK HOW SALMONELLA BEHAVES AT EACH STAGE. THIS WILL REVEAL WHICH TYPES (SEROTYPES) OF SALMONELLA ARE MOST COMMON AND WHETHER THEY POSE A PUBLIC HEALTH CONCERN.THE SECOND AIM IS TO USE THE DATA TO BUILD A NEW FRAMEWORK FOR UNDERSTANDING HOW SALMONELLA POPULATIONS BEHAVE DURING PROCESSING. BY ANALYZING BOTH MICROBIAL LEVELS AND SEROTYPES, RESEARCHERS WILL IDENTIFY PATTERNS THAT SHOW HOW PROCESSING STEPS LIKE WASHING, CHILLING, AND GRINDING AFFECT INTERNAL AND EXTERNAL CONTAMINATION DIFFERENTLY. FOR EXAMPLE, THEY WILL MODEL HOW MUCH CONTAMINATION IS REDUCED BY SURFACE TREATMENTS (SUCH AS SANITARY DRESSING) AND WHETHER INTERNAL CONTAMINATION RE-EMERGES LATER IN GROUND PRODUCT. THIS MODELING ALSO INCLUDES STATISTICAL TOOLS TO DETE,CT WHETHER CONTAMINATION IN ONE BIRD MIGHT BE LINKED TO ANOTHER, HELPING TO DETERMINE MICROBIAL INDEPENDENCE. THESE INSIGHTS CAN GUIDE SMARTER PROCESS CONTROLS AND LOTTING STRATEGIES, MAKING IT EASIER FOR PROCESSORS TO IDENTIFY WHEN A PROBLEM STARTS AND HOW FAR IT MIGHT SPREAD.THE THIRD AIM IS TO APPLY THIS FRAMEWORK IN REAL-WORLD SETTINGS TO IMPROVE HOW THE TURKEY INDUSTRY APPROACHES SALMONELLA CONTROL. THIS INCLUDES WORKING DIRECTLY WITH A COMMERCIAL TURKEY PROCESSOR TO TEST THE FRAMEWORK UNDER PRODUCTION CONDITIONS, AND ORGANIZING COLLABORATIVE SESSIONS WITH INDUSTRY STAKEHOLDERS. THESE CO-CREATION MEETINGS WILL ENSURE THAT RECOMMENDATIONS ARE NOT ONLY SCIENTIFICALLY SOUND BUT ALSO REALISTIC AND USEFUL FOR PROCESSORS. THE ULTIMATE GOAL IS TO CREATE DATA-DRIVEN STRATEGIES THAT ALLOW THE TURKEY INDUSTRY TO TAKE A MORE PROACTIVE ROLE IN FOOD SAFETY, IDENTIFYING THE MOST CRITICAL POINTS FOR INTERVENTION AND TARGETING EFFORTS WHERE THEY WILL HAVE THE GREATEST IMPACT.THIS WORK IS CARRIED OUT BY A MULTIDISCIPLINARY TEAM WITH EXPERTISE IN MICROBIOLOGY, EPIDEMIOLOGY, POULTRY SCIENCE, DATA SCIENCE, AND FOOD SAFETY REGULATION. THE TEAM'S COMBINED EXPERIENCE ENSURES THAT FINDINGS WILL BE SCIENTIFICALLY RIGOROUS, PRACTICALLY RELEVANT, AND TAILORED TO MEET THE NEEDS OF BOTH REGULATORS AND INDUSTRY. BY COMBINING LABORATORY METHODS, STATISTICAL MODELING, AND INDUSTRY COLLABORATION, THIS PROJECT AIMS TO TRANSFORM HOW WE UNDERSTAND AND MANAGE SALMONELLA IN TURKEY PRODUCTION. IT WILL PROVIDE KEY DATA THAT CAN SHAPE SMARTER REGULATIONS, REDUCE FOODBORNE ILLNESS, AND MAKE TURKEY PRODUCTS SAFER FOR CONSUMERS.
$650,000Kansas State University · · FY2025 · National Institute of Food and Agriculture
IMPROVING WHEAT YIELD POTENTIAL TO MEET FUTURE DEMANDS FOR FOOD WHILE REDUCING LAND USE AND PROMOTING SUSTAINABLE FARMING REQUIRES CONCERTED EFFORTS OF RESEARCH AND BREEDING COMMUNITY AIMED AT 1) DISCOVERING GENETIC VARIANTS CONTROLLING PHYSIOLOGICAL AND DEVELOPMENTAL PROCESSES THAT CONTRIBUTE TO TRAITS OF AGRONOMIC IMPORTANCE, ESPECIALLY PRODUCTIVITY AND NUTRITIONAL VALUE TRAITS, 2) EVALUATING THE PHENOTYPIC EFFECTS OF INDIVIDUAL AND STACKED BENEFICIAL VARIANTS IN ADAPTED GERMPLASM UNDER REALISTIC AGRICULTURAL SETTINGS, AND THEN 3) USING THIS KNOWLEDGE TO REDESIGN WHEAT PLANT BY STRATEGICALLY COMBINING THOSE ALLELES WHOSE INTERACTION GREATLY ENHANCES TRAIT EXPRESSION. WHILE PROGRESS WAS MADE TOWARDS IDENTIFYING THE GENETIC BASIS OF MANY AGRONOMIC TRAITS, WE STILL NEED TO EXPAND GENETIC DIVERSITY ACCESSIBLE FOR BREEDING BY EITHER INTRODUCING NEW DIVERSITY FROM THE ANCESTRAL POPULATIONS OF WILD RELATIVES OR LANDRACES, OR BY CREATING NOVEL VARIANTS BY MUTAGENESIS OR GENE EDITING. WE STILL HAVE LIMITED UNDERSTANDING OF HOW DISCOVERED GENES AND THEIR ALLELES WILL PERFORM IN DIVERSE GENETIC BACKGROUNDS OR ENVIRONMENTS, OR HOW TRAITS AFFECTING DIFFERENT ASPECTS OF WHEAT BIOLOGY WILL INTERACT WITH EACH OTHER ONCE COMBINED. TO ADDRESS THESE CHALLENGES, URGENT INTEGRATION OF INNOVATIVE TECHNOLOGIES AND BREEDING STRATEGIES INTO THE TRANSLATIONAL ACTIVITIES IS REQUIRED.OVER THE LAST DECADE, BY THE EFFORTS OF NATIONAL AND INTERNATIONAL WHEAT RESEARCH PROGRAMS, THE GENETIC BASIS OF MANY AGRONOMIC TRAITS HAS BEEN ESTABLISHED. THE RELEASE OF THE ANNOTATED WHEAT GENOME SEQUENCE AND DEVELOPMENT OF COMPARATIVE GENOMICS TOOLS AND RESOURCES PROVIDED A POWERFUL FRAMEWORK FOR EXTRAPOLATING GENE MAPPING INFORMATION FROM OTHER CROPS INTO WHEAT. THESE DISCOVERIES NOW PROVIDE UNIQUE OPPORTUNITY TO REDESIGN BIOLOGICAL PATHWAYS UNDERLYING MAJOR AGRONOMIC TRAITS IN WHEAT BY INTRODUCING THE FAVOURABLE ALLELES OF CAUSAL GENES INTO THE BREEDING PIPELINES.MANY STUDIES DEMONSTRATED THAT THE CRISPR-CAS SYSTEM CAN TAKE FULL ADVANTAGE OF THESE NEW GENOMIC RESOURCES AND FACILITATE THE CHARACTERIZATION AND DEPLOYMENT OF THE NOVEL GENE VARIANTS. OUR PROJECT WILL ESTABLISH PLANT BREEDING PARTNERSHIP INCLUDING KANSAS STATE UNIVERSITY (KSU) AND THE UNIVERSITY OF SASKATCHEWAN (CANADA) WITH THE AIM TO INTEGRATE THE CRISPR-CAS-BASED TECHNOLOGY INTO THE WHEAT PRE-BREEDING PIPELINES AND IMPROVE WHEAT PRODUCTIVITY AND NUTRITIONAL QUALITY TRAITS IN ADAPTED GERMPLASM. THIS PROJECT WILL BUILD ON THE RESOURCES GENERATED IN THE ON-GOING GENE EDITING PROJECTS CONDUCTED BY THE PROJECT DIRECTORS AND COLLABORATORS. THE PROJECT ACTIVITIES WILL BE INTEGRATED WITH THE WHEAT CAP AND IWYP PROJECTS, AND THE FUTURE NIFA IWYP WINTER WHEAT BREEDING INNOVATION HUB IN MANHATTAN (KS).HERE, WE WILL CREATE VARIATION IN THE PROTEIN CODING OR REGULATORY REGIONS OF GENES THAT CAN INFLUENCE PLANT GROWTH, SPIKE AND GRAIN DEVELOPMENT, AND NUTRIENT ACCUMULATION IN GRAIN. WE WILL MAKE USE OF CRISPR-EDITED WHEAT LINES GENERATED BY OUR TEAM IN A PREVIOUS PROJECT WITH MUTATIONS IN THE GENES AFFECTING GRAIN SIZE AND WEIGHT, AND NUMBER OF GRAINS PER HEAD.WE WILL TEST A NEW STRATEGY TO CREATE NOVEL PHENOTYPIC VARIATION BY MUTATING THE REGULATORY REGIONS OF GENES CONTROLLING NITROGEN UPTAKE, CARBON FIXATION, GROWTH AND NUTRIENT REMOBILIZATION. CURRENTLY, WE HAVE LIMITED UNDERSTANDING OF THE RANGE OF POSSIBLE QUANTITATIVE VARIATION FOR THESE TRAITS THAT CAN BE GENERATED BY EDITING THE REGULATORY REGIONS OF GENES. IN THIS PROJECT, WE WILL INVESTIGATE PHENOTYPES PRODUCED BY THE CRISPR-CAS9-EDITED PROMOTER VARIANTS AND ASSESS THEIR UTILITY FOR WHEAT IMPROVEMENT. BY COMBINING AND TESTING DIFFERENT REGULATORY VARIANTS OF GENES SHOWING DIFFERENT LEVELS OF EXPRESSION, WE WILL IDENTIFY OPTIMAL COMBINATIONS OF VARIANTS SUPPORTING EFFECTIVE NITROGEN UPTAKE AND PLANT GROWTH.THE IDENTIFICATION AND ASSEMBLY OF POSITIVELY INTERACTING COMBINATIONS OF GENES AFFECTING WHEAT PRODUCTIVITY TRAITS HAS A POTENTIAL TO SUBSTANTIALLY INCREASE YIELD. ONE OF THE PRE-REQUISITES FOR THE IMPLEMENTATION OF THIS STRATEGY IS THE IDENTIFICATION OF ALLELES THAT DO NOT SHOW NEGATIVE INTERACTION. BY TRANSFERRING THE CRISPR-EDITED GENE VARIANTS INTO THE ADAPTED GERMPLASM ALREADY SELECTED FOR OTHER BENEFICIAL AND COMPLEMENTARY TRAITS (E.G. HIGH BIOMASS, IMPROVED PLANT ARCHITECTURE, ETC.), THIS PROJECT WILL INVESTIGATE THE UTILITY OF QUANTITATIVE TRAIT VARIATION INDUCED BY GENE EDITING FOR WHEAT IMPROVEMENT, AND IDENTIFY THE COMBINATIONS OF GERMPLASM AND CRISPR-CAS INDUCED ALLELES CAPABLE OF SUPPRESSING NEGATIVE INTERACTION AMONG THE WHEAT PRODUCTION TRAITS.WITH GENE EDITING BECOMING ONE OF THE VALUABLE ASSETS IN THE BREEDER'S CROP IMPROVEMENT TOOLBOX, TRAINING OPPORTUNITIES FOR GRADUATE STUDENTS AND POSTDOCTORAL RESEARCHERS THAT INTEGRATE GENE EDITING SYSTEM WITH BREEDING METHODOLOGIES, BIOINFORMATICS, COMPARATIVE GENOMICS, AND MOLECULAR GENETICS ARE REQUIRED.IN OUR PROJECT, WE WILL DEVELOP EDUCATIONAL MODULES FOR POSTDOCTORAL RESEARCHERS AND PHD STUDENTS TO PROVIDE TRAINING IN APPLICATIONS OF GENE EDITING TECHNOLOGY FOR CROP IMPROVEMENT, REGULATORY ASPECTS OF CROP BIOTECHNOLOGY AND SCIENCE COMMUNICATION. THESE TRAINING IS INTENDED TO PREPARE THE NEW GENERATION OF CROP SCIENTISTS TO FACE THE CHALLENGES OF COMMUNICATING THEIR DISCOVERIES TO THE PUBLIC, CONSUMER GROUPS AND POLICYMAKERS TO ENSURE THAT THE SCIENCE-BASED POLICIES PREVAIL.
$650,000Kansas State University · · FY2020 · National Institute of Food and Agriculture
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** A BETTER UNDERSTANDING OF THE RELATIONSHIP BETWEEN THE GENOTYPE AND THE PHENOTYPE IS CRITICAL FOR IMPROVING PRODUCTIVITY AND ANIMAL WELL-BEING, OPTIMIZING ECONOMIC RETURN AND SUSTAINABILITY FOR PRODUCERS, AND INCREASING THE QUALITY OF POULTRY PRODUCTS FOR CONSUMERS. BECAUSE MOST (>90%) OF THE GENETIC MARKERS USED IN GENOMIC SELECTION ARE FOUND IN NON-CODING REGIONS OF THE GENOME, LINKING THESE VARIANTS TO RELEVANT GENES OR PATHWAYS IS NOT TRIVIAL. THE GOAL OF THIS PROPOSAL IS TO DEVELOP AND VALIDATE USER-FRIENDLY CRISPR (CLUSTERED, REGULARLY INTERSPACED, SHORT PALINDROMIC REPEAT)-BASED IN VITRO SCREENING RESOURCES FOR CHICKENS, INCLUDING CELL LINES, DNA VECTORS, PROTOCOLS, DATA, AND TRAINING, AND SHARE THESE RESOURCES WITH THE RESEARCH COMMUNITY. WE WILL ACHIEVE THIS THROUGH ESTABLISHING AND OPTIMIZING IN VITRO CRISPR-BASED TRANSCRIPTION ACTIVATION (CRISPRA) AND CRISPR-BASED TRANSCRIPTION REPRESSION (CRISPRI) SYSTEMS IN DIFFERENT LINEAGES OF CHICKEN CELL LINES, FOLLOWED BY HIGH-THROUGHPUT SINGLE-CELL-BASED CRISPR FUNCTIONAL SCREENING. OUTCOMES OF THIS PROPOSAL WILL INCLUDE A MORE APPROACHABLE IN VITRO PLATFORM FOR TESTING THE FUNCTION OF GENES AND REGULATORY ELEMENTS OF INTEREST IN A HIGH-THROUGHPUT MANNER. THIS WILL CONTRIBUTE TO BROADENING THE SCOPE OF POULTRY GENOMICS RESEARCH FROM ASSOCIATION-BASED FINDINGS TO MECHANISTIC PRINCIPLES OF GENE REGULATION, BRINGING US ONE STEP CLOSER TO CONNECTING GENOME TO PHENOME.
$650,000The Pennsylvania State University · · FY2023 · National Institute of Food and Agriculture
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** POTATO (SOLANUM TUBEROSUM) IS THE THIRD MOST-CONSUMED FOOD CROP IN THE WORLD GROWN IN ROUGHLY 130 COUNTRIES, 95 OF WHICH ARE DEVELOPING COUNTRIES. POTATO IS CONSIDERED A FOOD SECURITY CROP FOR DEVELOPING NATIONS, IN WHICH POTATO PRODUCTION HAS RAMPED UP SUBSTANTIALLY OVER THE PAST 30 YEARS. THE US REMAINS THE FOURTH LARGEST PRODUCER OF POTATOES IN THE WORLD. POTATO IS A TOP FIVE COMMODITY CROP IN THE PDS' HOME STATE OF NEVADA. POTATO HAS AN ANNUAL VALUE OF $4.2 BILLION IN THE USA. UP TO 33% OF THE US POTATO TUBER CROP IS LOST DUE TO POSTHARVEST ISSUES LIKE WOUNDING, BRUISING, AND PATHOGEN ATTACK. THESE LOSSES CAN REACH AS HIGH AS 50% IN DEVELOPING NATIONS DUE TO FACTORS SUCH AS LACK OF PROPER STORAGE CONDITIONS. THE ECONOMIC IMPORTANCE OF POTATO EXTENDS BEYOND THE VALUE OF THE HARVESTED CROP. THE GLOBAL POTATO PROCESSING MARKET IS CURRENTLY VALUED AT $27.4 BILLION AND IS EXPECTED TO GROW TO $35.4 BILLION BY 2026.IT IS WELL KNOWN THAT WOUND HEALING AND DEPOSITION OF THE CORKY MATERIAL SUBERIN IN WOUND SITES IS OF CRITICAL IMPORTANCE TO THE THE POST-HARVEST STORAGE LIFE OF POTATOES. WOUND SUBERIN DEPOSITION CAPACITY AND STORAGE LIFE ARE POSITIVELY CORRELATED. THAT IS TO SAY, POTATO CULTIVARS WITH BETTER WOUND SUBERIN DEPOSITION CAPACITY TEND TO HAVE LONGER STORAGE LIFE. EFFORTS TO IMPROVE WOUND HEALING CAPACITY OF THE USA'S NUMBER ONE VEGETABLE CROP MUST BE INFORMED BY UNDERSTANDING THE FUNDAMENTAL PROCESSES THAT GOVEN WOUND HEALING. THIS CAN BE ACCOMPLISHED BY UNDERSTANDING THE GENES AND SIGNALING PROCESSES THAT GOVERN WOUND SUBERIN DEPOSITION. THISRESEARCH PROJECT AIMS TO DO EXACTLY THAT, TO PROVIDE AN IN-DEPTH UNDERSTANDING OF THE GENETIC AND SIGNALING FACTORS THAT REGULATE WOUND HEALING IN A CROP SPECIES IMPORTANT FORNATIONAL AND GLOBAL FOOD SECURITY, POTATO.TO ACCOMPLISH THIS, CUTTING-EDGE GENOMICS AND GENOME EDITING TECHNIQUES, SUCH AS CRISPR AND HIGH-THROUGHPUT DNA/RNA SEQUENCINGWILL BE EMPLOYED. THROUGH THIS WORK, POTATO GERMPLASM WITH ALTERED WOUND SUBERIN DEPOSITION CAPACITY WILL BE GENERATED. THE GERMPLASM AND INFORMATION GENERATED THROUGH THIS RESEARCH WILL PROVIDE A VALUABLE RESOURCE FOR PLANT BREEDING EFFORTS AIMED AT REDUCING POST-HARVEST POTATO TUBER LOSSESTHAT WILL TRANSLATABLE TO OTHER CROPS THAT UNDERGO LONG-TERM STORAGE (E.G. SWEET POTATO, RUTABAGA, CARROT, ETC.).
$650,000Board Of Regents Of Nevada System Of Higher Education · · FY2024 · National Institute of Food and Agriculture
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** THE BEEF AND DAIRY INDUSTRY ARE THE PRIMARY SUPPLIERS OF DIETARY PROTEIN SOURCES AIDING TO MEET THE GROWING GLOBAL DEMAND, WHICH RELIES HEAVILY ON ANIMAL REPRODUCTION AS A CENTRAL DETERMINANT OF PRODUCTION EFFICIENCY. UNFORTUNATELY, DAIRY CATTLE FERTILITY IS CONTINUALLY DECLINING WORLDWIDE, WHICH HAMPERS IMPLEMENTED EFFORTS TO ADVANCE ECONOMIC EFFICIENCY IN THE DAIRY SECTOR. EVEN THOUGH ASSISTED REPRODUCTIVE TECHNOLOGIES (ARTS) CURRENTLY ACCOUNT FOR A GROWING PORTION OF LIVESTOCK AND HUMAN POPULATIONS, ART-INDUCED OXIDATIVE STRESS CAN ALSO CONTRIBUTE TO IMPACTS ON EMBRYO DEVELOPMENT WITH LONG-TERM CONSEQUENCES ON FETAL GROWTH AND OFFSPRING HEALTH. AMONG THE SEVERAL MOLECULAR MECHANISMS ASSOCIATED WITH AN EMBRYO'S RESPONSE TO STRESS, WE HAVE PREVIOUSLY FOUND THE NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR 2 - KELCH-LIKE ECH-ASSOCIATED PROTEIN 1 (NRF2-KEAP1) PATHWAY TO ACT AS A FIRST-LINE DEFENSE MECHANISM, WITH THE ABILITY OF THE EMBRYO TO ACTIVATE ITS NRF2-SIGNALING HIGHLY ASSOCIATED WITH ITS SURVIVAL AND VIABILITY UNDER OXIDATIVE STRESS CONDITIONS. THEREFORE, APPROACHES TO INDUCE NRF2 PROTEIN ACTIVITY IN BOVINE EMBRYOS WILL CONTRIBUTE TO IMPROVED SURVIVAL, VIABILITY, AND ENHANCED REPRODUCTIVE EFFICIENCIES FOLLOWING ARTS IN ANIMALS. MOREOVER, HERE WE AIM TO IMPLEMENT A DUAL-TARGETED APPROACH USING CRISPR-CAS9 MEDIATED KNOCKDOWN OF KEAP1 GENE (NRF2-INHIBITOR) IN SPECIFIC AIM 1 AND A PHARMACOLOGICAL STRATEGY USING THE ANTIOXIDANT QUERCETIN IN THE IN VITRO CULTURE SYSTEM IN SPECIFIC AIM 2, TO INCREASE THE RELATIVE ABUNDANCE OF LOCALIZED NRF2 PROTEIN IN BOVINE PREIMPLANTATION EMBRYOS TO PROMOTE THEIR SURVIVAL AND VIABILITY UNDER OXIDATIVE STRESS CONDITIONS. THE MODULATION OF THIS PATHWAY IN PREIMPLANTATION EMBRYOS IS EXPECTED TO REDUCE OXIDATIVE DAMAGE IN THE RESULTING BLASTOCYSTS TO ENHANCE THE SURVIVAL AND VIABILITY OF EMBRYOS UNDER SUBOPTIMAL CONDITIONS, PARTICULARLY WITH RESPECT TO CURRENTLY IMPLEMENTED ART PROCEDURES. MOREOVER, THE ACTIVATION OF NRF2 IN THE PREIMPLANTATION PERIOD HAS THE POTENTIAL TO MITIGATE STRESS-INDUCED ABNORMAL MOLECULAR ALTERATIONS, THEREBY ATTENUATING LONG-TERM NEGATIVE CONSEQUENCES OF STRESS ON FETAL GROWTH AND OFFSPRING HEALTH. THE PLANNED PROJECT WILL IDENTIFY GENES AND GENOME REGIONS, THAT CAN BE TARGETS FOR FUTURE INTERVENTION AGAINST PHYSIOLOGICAL AND ENVIRONMENTAL STRESS-INDUCED INFERTILITY PROBLEMS IN DAIRY AND BEEF CATTLE.
$650,000Colorado State University · · FY2024 · National Institute of Food and Agriculture
RUI: DNA replication in Chlamydomonas reinhardtii
$650,000Amy Ikui · Cuny Brooklyn College · · FY2025 · BIO
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** THIS PROPOSAL IS GOING TO ADDRESS AREA 1: MANAGEMENT PRACTICES TO MINIMIZE ENVIRONMENTAL RISK OF GE ORGANISMS OF THE USDA BRAG STANDARD RESEARCH PROGRAM. A PROBLEM IN TOMATO IS THE SUBSTANTIAL POLLEN-MEDIATED GENE FLOW (PMGF) FROM GENETICALLY ENGINEERED (GE) TOMATO TO NON-GE TOMATO. USING ARABIDOPSIS AS A PROOF-OF-CONCEPT RESEARCH, WE SEEK TO GENETICALLY ENGINEER TOMATO PRODUCING CRISPR POLLEN THAT CAN DESTROYSEEDDEVELOPMENT IN THE NON-GE PLANTS AFTER DOUBLE FERTILIZATION. THE GE LINES WILL BE USED FOR SELFING OR RECIPROCAL CROSSING WITH THE NON-GE ARABIDOPSIS OR TOMATO PLANTS TO TEST THE EFFECT ONSEED ABORTION. THE BEST LINE IN TOMATO WILL BE USED FOR FIELD TRIALS TO TEST PHENOTYPIC STABILITY AND INTERFERENCE WITH SEED PRODUCTION, AND MEASURE THE EXTENT OF PMGF FROM THE GE TO NON-GE PLANTS IN A REAL WORLD SETTING. THE EXPECTED OUTCOME OF THIS PROJECT IS THE ENGINEERED CRISPR POLLEN IN EACH SPECIES, WHICH IN TURN WILL HIGHLY LIMIT PMGF FROM GE PLANTS WITHOUT INTERFERENCE WITH SEED PRODUCTION. IT COULD BE USED AS THE BACKGROUND SOURCE FOR GENETIC ENGINEERING OF ANY TRAITS. THEREFORE, THE RESEARCH DIRECTLY ADDRESSES KEY BARRIERS TO THE DEVELOPMENT OF REGULATORY AND BEST MANAGEMENT PLANS AND SUSTAINABLE PRODUCTION IN TOMATO, WHICH COULD BE APPLICABLE TO OTHER GE CROPS.
$650,000North Carolina State University · · FY2023 · National Institute of Food and Agriculture
** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** THE PROJECTMECHANISMS OF COLD STRESS TOLERANCE RESPONSES IN RICE BY PROGRAM DIRECTOR (PD) MICHAEL SCHLÄPPI FROM MARQUETTE UNIVERSITY AND CO-PDS GEORGIA EIZENGA & JEREMY EDWARDS FROM USDA-ARS FITS THE A1152 PROGRAM AREA PRIORITY MECHANISMS OF PLANT RESPONSES TO ABIOTIC STRESSES. RICE YIELD WOULD BE ENHANCED IF GROWERS COULD PLANT TWO WEEKS EARLIER IN THE SEASON TO BETTER UTILIZE THE SPRING RAIN AND AVOID HIGH NIGHTTIME TEMPERATURES OF SUMMER, WHICH DECREASE GRAIN QUALITY AND YIELD. OUR OBJECTIVE IS TO USE THE WELL-CHARACTERIZED RICE DIVERSITY PANEL 1 (RDP1) COLLECTION OF 424 ACCESSIONS AND FOUR SUBPOPULATION-SPECIFIC RECOMBINANT INBRED LINE AND NEAR ISOGENIC LINE POPULATIONS SPECIFICALLY GENERATED FOR THIS PROJECT TO PROVIDE A BETTER UNDERSTANDING OF THE BASIC GENETIC MECHANISMS RESPONSIBLE FOR TOLERANCE TO COLD TEMPERATURES AT THE EARLY GROWTH STAGES IN RICE. TO ACCOMPLISH THIS, WE WILL (I) USE DIFFERENT RECOMBINANT INBRED LINES TO DETERMINE GENETIC MECHANISMS OF RICE COLD TOLERANCE RESPONSES BY IDENTIFYING COLD-TOLERANT-PARENT-SPECIFIC GENE CO-EXPRESSION MODULES AND THEIR CENTRAL HUB (CONTROL) GENES; (II) ASSOCIATE MODULES AND HUB GENES TO PREVIOUSLY MAPPED QUANTITATIVE TRAIT LOCI (QTL) FOR COLD TOLERANCE; (III) FINE-MAP QTL FOR COLD TOLERANCE WE PREVIOUSLY IDENTIFIED USING THE RDP1 AND FOUR RICE INBRED POPULATIONS TO NARROW DOWN COLD TOLERANCE CANDIDATE GENES; (IV) IDENTIFY CANDIDATE GENES WITHIN THE MAPPED QTL FOR COLD TOLERANCE; AND (V) VALIDATE SELECTED CANDIDATE GENES IN TRANSGENIC PLANTS WITH THE CRISPR GENE KNOCKOUT AND STRONG PROMOTER OVEREXPRESSION SYSTEMS. THIS INFORMATION WILL BE THE BASIS FOR GENE DISCOVERY AND IDENTIFICATION OF COLD-ASSOCIATED DNA MARKERS, AND ULTIMATELY RICE GERMPLASM WITH IMPROVED SEEDLING VIGOR FOR USE IN CULTIVAR DEVELOPMENT PROGRAMS.
$650,000Marquette University · · FY2023 · National Institute of Food and Agriculture