MULTI CRYSTAL EXPERIMENTS WITH INSULIN

$13,408P41FY2010RRNIH

Brookhaven Science Assoc-Brookhaven Lab, Upton NY

Investigators

Linked publications & trials

Paper 37485824 Paper 37058079 Paper 35787556 Paper 34709351 Paper 31908351 Paper 31230945 Paper 31204686 Paper 31167793 Paper 31045565 Paper 30802506 Paper 29946976 Paper 29593097 Paper 28608671 Paper 28442575 Paper 28250468 Paper 28033710 Paper 28026167 Paper 27871088 Paper 27806260 Paper 27452459 Paper 27447429 Paper 27297880 Paper 27285058 Paper 27135925 Paper 26996959 Paper 26884182 Paper 26574563 Paper 26524494 Paper 26494017 Paper 26447926 Paper 26411795 Paper 26342082 Paper 26323303 Paper 26282925 Paper 26214585 Paper 26147157 Paper 26027487 Paper 26013825 Paper 25894083 Paper 25864631 Paper 25806409 Paper 25802330 Paper 25747658 Paper 25702872 Paper 25615864 Paper 25600932 Paper 25581168 Paper 25484231 Paper 25423286 Paper 25383759 Paper 25343789 Paper 25184411 Paper 25165982 Paper 25123481 Paper 25087510 Paper 25043052 Paper 24988328 Paper 24927729 Paper 24888261 Paper 24862282 Paper 24813203 Paper 24769418 Paper 24666693 Paper 24648526 Paper 24616444 Paper 24598752 Paper 24598733 Paper 24591642 Paper 24585883 Paper 24531463 Paper 24527857 Paper 24462099 Paper 24398681 Paper 24362703 Paper 24116139 Paper 24047895 Paper 23975200 Paper 23967305 Paper 23955046 Paper 23717209 Paper 23451136 Paper 23432095 Paper 23416107 Paper 23396446 Paper 23322043 Paper 23256532 Paper 23192041 Paper 23167435 Paper 23055931 Paper 23043139 Paper 23000721 Paper 22940583 Paper 22908299 Paper 22879982 Paper 22820988 Paper 22802670 Paper 22691781 Paper 22659319 Paper 22632835 Paper 22605381

Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Acoustic droplet ejection (ADE) was used to transfer 2.5uL droplets of crystal slurries from 396 well plates onto MiteGen micro mesh pins. Multiple homogenous slurries will be tested to verify that acoustic specimen preparation is effective and non-damaging for R3 insulin and P43212 lysozyme crystals of different sizes, different concentrations, and different overall purity and cleanliness. All tested conditions yielded accurately transferred droplets of precise volumes. We will show that transferred droplets yield undamaged and well diffracting crystals, with diffraction patterns and the resulting structures comparable for ADE mounted and hand mounted specimens.

View original record on NIH RePORTER →