NOVEL METHODOLOGY FOR STUDYING OXIDATIVE METABOLISM

$8,672P41FY2010RRNIH

University Of Pennsylvania, Philadelphia PA

Investigators

Linked publications & trials

Paper 37905305 Paper 36695294 Paper 31502710 Paper 30680976 Paper 29763672 Paper 29751289 Paper 28824123 Paper 28767621 Paper 28239652 Paper 27544505 Paper 27460711 Paper 27370153 Paper 27347478 Paper 27222794 Paper 27140763 Paper 26901812 Paper 26350896 Paper 26303178 Paper 26207147 Paper 26120204 Paper 26015810 Paper 25996891 Paper 25844314 Paper 25762748 Paper 25311569 Paper 25263944 Paper 25236594 Paper 25187033 Paper 25099319 Paper 25063755 Paper 24967878 Paper 24834022 Paper 24614912 Paper 24443217 Paper 24439328 Paper 24416473 Paper 24338944 Paper 24244206 Paper 24179814 Paper 24143268 Paper 24029054 Paper 23852501 Paper 23770408 Paper 23745147 Paper 23708193 Paper 23685226 Paper 23664401 Paper 23653267 Paper 23400938 Paper 23392384 Paper 23359872 Paper 23304651 Paper 23243386 Paper 23142069 Paper 23138407 Paper 23106049 Paper 23028560 Paper 22879054 Paper 22879012 Paper 22879011 Paper 22789842 Paper 22624014 Paper 22535702 Paper 22502579 Paper 22431193 Paper 22426208 Paper 22302557 Paper 22281486 Paper 22270722 Paper 22259099 Paper 22259098 Paper 22246782 Paper 22230285 Paper 22213239 Paper 22135087 Paper 22129642 Paper 22128105 Paper 22094481 Paper 22034913 Paper 22018493 Paper 22009759 Paper 22006897 Paper 21911412 Paper 21908126 Paper 21863374 Paper 21613872 Paper 21608026 Paper 21448961 Paper 21445789 Paper 21445780 Paper 21445779 Paper 21358489 Paper 21333466 Paper 21325076 Paper 21317356 Paper 21212364 Paper 21191380 Paper 20951217 Paper 20924593 Paper 20865753

Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The metabolic demands of the brain are met almost exclusively through metabolism of glucose. Under normal conditions, the brain consistently extracts about 50% of the oxygen in the blood. The changes in metabolic demand are matched by changes in blood flow rather than by changes in oxygen extraction. Regional variations in oxidative metabolism typically correlate well with regional variations in blood flow. The correlation is the rationale for substituting blood flow maps for oxygen consumption maps. There are no clinically used methods for the detection of oxidative metabolism in-vivo though metabolic disturbances in many diseases are important. In the past, in order to have a suitable model, 17O MRI techniques are modeled in swine to measure hemispheric cerebral metabolic rate of oxygen consumption (CMRO2) by detection of metabolically produced H217O by rapid T1[unreadable]-wieghted proton magnetic resonance imaging on a 1.5 T clinical scanner. The 17O is delivered as oxygen gas by a custom, minimal-loss, precision delivery breathing circuit and converted to H[unreadable]217O by oxidative metabolism. A high temporal resolution pulse sequence is employed to measure CMRO2. Proton measurements of signal change due to metabolically produced water are correlated with 17O in-vivo measurements. Using these techniques, the hemispheric CMRO2 in swine is estimated to be 1.23 [unreadable]0.26 [unreadable]mol/g/min;consistent with existing literature values. In the present study, we employed diffuse reflectance and correlation spectroscopy to monitor the response of cerebral oxygenation and blood flow to hypercapnia in swine and to compare oxygen consumption optically estimated with17O MRI measurements.

View original record on NIH RePORTER →