TOP-DOWN MASS SPECTROMETRY FOR THE IDENTIFICATION OF PTM ENSEMBLES

Washington University, Saint Louis MO

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Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many papers are published that employ bottom-up proteomics to identify sites of potentail phosphorylation or other posttranslational modification. It is important to know the total ensemble of PTM for a protein at a particular time in the cell cycle. This information cannot be gleaned from the bottom-up strategy. Top down proteomics will be used to interrogate large parts of a protein (>10kDa) to discover the distributions of PTM during on proteins isolated from cells. The workflow will be developed by studying Chk2, an autophosphorylating kinase with more than 30 sites of phosphorylation. We will map out the distribution of phosphorylation from Chk2 captured at various times during the cell cycle.