Analytical Core/Richard B. van Breemen

$238,674P50FY2010ATNIH

University Of Illinois At Chicago, Chicago IL

Investigators

Linked publications & trials

Paper 39447601 Paper 37762540 Paper 37535691 Paper 37482264 Paper 37253812 Paper 36744762 Paper 36328482 Paper 35616633 Paper 34461730 Paper 34416305 Paper 33251548 Paper 33241325 Paper 33197178 Paper 33049645 Paper 32804502 Paper 32679162 Paper 32463230 Paper 32364634 Paper 32285669 Paper 32258409 Paper 32108467 Paper 31913227 Paper 31887327 Paper 31744669 Paper 31554401 Paper 31478376 Paper 31307793 Paper 30981705 Paper 30961965 Paper 30608650 Paper 30287984 Paper 30287983 Paper 30287522 Paper 30063944 Paper 29699871 Paper 29651231 Paper 29641206 Paper 29513526 Paper 29474979 Paper 29175549 Paper 29127460 Paper 28985473 Paper 28966697 Paper 28964928 Paper 28899509 Paper 28812892 Paper 28774812 Paper 28647482 Paper 28558170 Paper 28506498 Paper 28392269 Paper 28067513 Paper 27966173 Paper 27802026 Paper 27795590 Paper 27696846 Paper 27677719 Paper 27636306 Paper 27617882 Paper 27269377 Paper 27233988 Paper 27182123 Paper 27180381 Paper 27118320 Paper 26760374 Paper 26680272 Paper 26505758 Paper 26473469 Paper 26438626 Paper 26393937 Paper 26285764 Paper 26244884 Paper 26177360 Paper 26134484 Paper 26125082 Paper 25859589 Paper 25844270 Paper 25774910 Paper 25437744 Paper 25346613 Paper 25295852 Paper 25080313 Paper 25045111 Paper 24895010 Paper 24861737 Paper 24817649 Paper 24673652 Paper 24484881 Paper 24342125 Paper 24223609 Paper 24192160 Paper 24077106 Paper 23968665 Paper 23884629 Paper 23874474 Paper 23850540 Paper 23676633 Paper 23513960 Paper 23512484 Paper 23451393

Abstract

This Analytical core will be to provide essential mass spectrometry (MS) and nuclear magnetic resonance (NMR) measurements in support of all Projects and other Cores of the Botanical Center. These measurements will include routine analytical studies as well as the development of new analytical methods to support evolving research within the Center. Analytical Core personnel will provide expertise for the design and interpretation of analytical measurements for all Cores and Projects within the Center. During the first 10 years of the Botanical Center, the analytical measurements withing this core were based on mass spectrometry and have included high resolution accurate mass measurements, liquid chromatography-mass spectrometry (LC-MS), tandem mass spectrometry (MS-MS), LC-MS-MS, and pulsed ultrafiltration LC-MS (a technique invented in this laboratory for rapidly identifying ligands to pharmacological targets). In addition to providing these mass spectrometry-based analytical techniques, the analytical capabilities of this core will be expanded to include routine proton and carbon 13 ID and 2D NMR and high field NMR using 600, 800 and 900 MHz instruments. Therefore, this core will be able to provide a more complete range of analytical measurements. Several analytical assays have been developed in the projects during the previous funding period, and this core will provide these new assays as services to all Projects and Cores during the next funding period. Examples of some measurements that will be provided include high resolution 1D and 2D NMR, high resolution accurate mass LC-MS measurements for the determination of elemental compositions, MS-MS fragmentation pattern studies in support of structure elucidation in Project 1, Project 3, Core B, and Core C, and ultrafiltration-LC-MS screening assays. As needed, new MS and NMR methods will be developed and implemented for the detection, characterization and quantitative analysis of botanical natural products and their metabolites in botanical extracts and biological fiuids. In summary, this core will provide MS and NMR measurements and analytical expertise to enhance the productivity of all Projects and Cores in the Center.

View original record on NIH RePORTER →