GLYCOPROTEINS EXPRESSING GLYCANS THAT REACT WITH DBA AND ANTI-POLY SIALIC ACID

$126,714P41FY2009RRNIH

University Of Georgia, Athens GA

Investigators

Linked publications & trials

Paper 30873836 Paper 30463938 Paper 28928219 Paper 26899007 Paper 26440046 Paper 25737452 Paper 25726973 Paper 25662311 Paper 25416783 Paper 25365792 Paper 25299151 Paper 25253346 Paper 25190709 Paper 24664283 Paper 24524620 Paper 24510673 Paper 24412195 Paper 24026681 Paper 23836288 Paper 23814498 Paper 23708607 Paper 23580612 Paper 23552074 Paper 23350991 Paper 23329833 Paper 23296534 Paper 23174105 Paper 23115008 Paper 23072385 Paper 23061027 Paper 22988249 Paper 22919114 Paper 22904325 Paper 22817996 Paper 22735313 Paper 22722607 Paper 22712033 Paper 22683610 Paper 22665489 Paper 22654114 Paper 22556407 Paper 22446837 Paper 22364583 Paper 22308406 Paper 22167197 Paper 22084120 Paper 22046029 Paper 22045358 Paper 21945038 Paper 21855080 Paper 21812486 Paper 21740066 Paper 21620696 Paper 21613225 Paper 21606487 Paper 21598385 Paper 21460210 Paper 21356517 Paper 21078982 Paper 21057479 Paper 20816498 Paper 20816222 Paper 20816160 Paper 20727200 Paper 20625600 Paper 20564262 Paper 20507986 Paper 20087802 Paper 20054389 Paper 20044576 Paper 19969597 Paper 19953551 Paper 19786568 Paper 19545112 Paper 19526562 Paper 19449840 Paper 19379697 Paper 19072240 Paper 18690643 Paper 18411279 Paper 18281150 Paper 18271524 Paper 18230362 Paper 18047270 Paper 17915907 Paper 17911917 Paper 17465008 Paper 17384119 Paper 17284602 Paper 17279607 Paper 17244542 Paper 17137339 Paper 17088359 Paper 17046705 Paper 17038624 Paper 16854399 Paper 16697346 Paper 16606368 Paper 16512686 Paper 16259040

Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Stem Cell Program (Dalton) has identified Dolichos biflorus agglutinin (DBA) positive epitopes on ES cells that disappear when differentiation to EPL and EBods is induced. Lectin blotting with DBA identified 6-10 glycoproteins expressing glycans recognized by DBA. The glycoproteins expressing these glycans are being identified by excision of gel slices, trypsinization, and MALDI-TOF MS analysis. Moreover, the glycans on these glycoproteins are released, tagged with pyridylamine, and identified by chromatographic separation and MALDI-TOF MS. Initial results suggest that the DBA epitopes are on N-linked glycans because of their sensitivity to N-glycans release. Ultimately, we will answer the question of how the DBA glycan reactivity disappears after differentiation. The Glycotranscriptome Program (Moremen) has identified polysialic acid synthase expression in differentiated (EBod) cells. Staining of EBod and not ES cells with antibody specific to polysialic acid confirms this result. We will identify the glycoproteins that express this glycan and their function.

View original record on NIH RePORTER →