Nanoparticle Delivery of Oligonucleotides Targeting Missense Mutations in Keratin

$48,537P30FY2009ARNIH

Northwestern University At Chicago, Evanston IL

Investigators

Linked publications & trials

Paper 39581457 Paper 37471166 Paper 37216502 Paper 36165649 Paper 35213752 Paper 34905516 Paper 34757068 Paper 34425055 Paper 34107301 Paper 33709070 Paper 33709017 Paper 33385397 Paper 33044803 Paper 32892377 Paper 32598088 Paper 32546661 Paper 32286303 Paper 32179593 Paper 32064044 Paper 32032578 Paper 32027876 Paper 31914679 Paper 31626841 Paper 31563153 Paper 31554739 Paper 31545611 Paper 31465738 Paper 31421125 Paper 31419300 Paper 31247200 Paper 31123999 Paper 30737086 Paper 30641038 Paper 30543901 Paper 30508584 Paper 30503001 Paper 30414395 Paper 30355494 Paper 30279169 Paper 30264418 Paper 30185650 Paper 29959233 Paper 29803800 Paper 29760388 Paper 29705292 Paper 29660300 Paper 29596905 Paper 29535305 Paper 29520027 Paper 29452119 Paper 29351356 Paper 29031600 Paper 28893859 Paper 28891468 Paper 28874417 Paper 28580931 Paper 28463230 Paper 28259685 Paper 28108301 Paper 27872138 Paper 27825105 Paper 27815408 Paper 27729281 Paper 27590073 Paper 27554821 Paper 27450726 Paper 27302902 Paper 27068470 Paper 26778564 Paper 26763418 Paper 26734587 Paper 26565021 Paper 26542580 Paper 26457733 Paper 26436695 Paper 26405826 Paper 26331337 Paper 26305708 Paper 26290388 Paper 26275995 Paper 26248284 Paper 26242300 Paper 26143398 Paper 25902507 Paper 25733715 Paper 25693896 Paper 25671465 Paper 25639731 Paper 25526033 Paper 25482622 Paper 25455687 Paper 25368015 Paper 25309616 Paper 25225338 Paper 25208567 Paper 25070955 Paper 24961930 Paper 24795843 Paper 24788983 Paper 24739758

Abstract

Controlling gene expression in primary cultured cells, such as human keratinocytes is difficult due to the stratum corneum, which ranges from 10-50 urn in thickness and serves as a barrier to the intracellular environment. Methods to introduce gene regulation agents such as oligonucleotides have met with limited success, as both the penetration depth and effectiveness of the regulation strategies vary. The proposed work will develop oligonucleotide-nanoparticle antisense agents as methods for controlling gene expression in keratinocytes. This work will take advantage of newly discovered conjugate properties of the nano-materials including enhanced stability, high penetration ability, and non-toxicity. The ability of these agent to work on genes of relevence in skin biology models will be tested.

View original record on NIH RePORTER →