PROJECT 4

$145,022P50FY2008GMNIH

University Of Pittsburgh At Pittsburgh, Pittsburgh PA

Investigators

Linked publications & trials

Paper 39680612 Paper 39645581 Paper 39632888 Paper 39447570 Paper 38645162 Paper 37816746 Paper 37772839 Paper 37766989 Paper 37679318 Paper 37560901 Paper 37406093 Paper 37330292 Paper 37230988 Paper 36868078 Paper 35913165 Paper 35421110 Paper 35167709 Paper 35150604 Paper 35022621 Paper 34915287 Paper 34136885 Paper 34105995 Paper 33901736 Paper 33863979 Paper 33758083 Paper 33722887 Paper 33648539 Paper 33368010 Paper 33332391 Paper 33262260 Paper 32901160 Paper 32796070 Paper 32747784 Paper 32685828 Paper 32401799 Paper 32363727 Paper 32152226 Paper 31986188 Paper 31815961 Paper 31676138 Paper 31525965 Paper 31471129 Paper 31463759 Paper 31435857 Paper 31422020 Paper 31415753 Paper 31413124 Paper 31320228 Paper 31254557 Paper 31227715 Paper 31167922 Paper 31155311 Paper 31125232 Paper 31076522 Paper 31068467 Paper 31019079 Paper 31019074 Paper 30947724 Paper 30916345 Paper 30871317 Paper 30853849 Paper 30847635 Paper 30567984 Paper 30352932 Paper 30349041 Paper 30333189 Paper 30225969 Paper 30181049 Paper 30087125 Paper 30069050 Paper 29756776 Paper 29695435 Paper 29643241 Paper 29579703 Paper 29577158 Paper 29498857 Paper 29176596 Paper 29151202 Paper 29079575 Paper 29072840 Paper 28929138 Paper 28905420 Paper 28794041 Paper 28732673 Paper 28528781 Paper 28221348 Paper 28202766 Paper 28181439 Paper 28167392 Paper 28093096 Paper 28059770 Paper 28001418 Paper 27958264 Paper 27858311 Paper 27840029 Paper 27701859 Paper 27571178 Paper 27356901 Paper 27354282 Paper 27335459

Abstract

Project IV: Nef [unreadable] Hck interaction (T. Smithgall, Cores C, D, and E) - Large amounts of soluble Nef for preliminary NMR evaluation have been obtained. The regulatory region of Hck (Hck 32L, containing the SH3 and SH2 domains as well as the SH2-kinase linker), has been expressed in E. coli with very high yield. When combined in an equimolar ratio, a single, stable Nef-Hck32L complex is formed. Preliminary conditions for Nef-Hck crystallization have been identified using developed protocols. - Hck32L has been triple-labeled and ongoing NMR experiments are expected to reveal chemical shift changes within the SH3 domain and elsewhere upon Nef binding.

View original record on NIH RePORTER →