CORE--Biochemical-Analysis Core

$240,072U19FY2007CANIH

University Of Southern California, Los Angeles CA

Investigators

Linked publications & trials

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Abstract

Core B is a biochemical analysis core that will provide indispensable services for all three projects. The biochemical component of Core B is designed to carry out protein purifications and to synthesize specialized DNA constructs required for Projects 1 and 3. The analysis component will be responsible for acquiring and performing global analysis on kinetic data, including presteady state stopped-flow fluorescence intensity and rotational anisotropy and rapid quench experiments, to be used in support of Projects 2 and 3. Core B will serve as a primary vehicle for training graduate and postdoctoral students in protein purification techniques and in the principles and practice of enzyme kinetic analysis.

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