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PURIFICATION, CHARACTERIZATION &FUNCTIONAL ANALYSIS OF YEAST ADA HAT COMPLEX

$3,280P41FY2000RRNIH

University Of Washington, Seattle WA

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Abstract

They are purifying Sst2 and Gpa1 from Saccharomyces cerevisiae with the goal of investigating how the Sst2-G protein interaction is regulated, and what domains of Sst2 mediate this interaction. In the course of their experiments, they observed a marked decrease in the electrophoretic mobility of Gst tagged Sst2 purified from pheromone-treated vs. untreated cells and will be evaluating the likely role of protein phosphorylation as the modification event attendant those observed mobility changes.

View original record on NIH RePORTER →