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FLUORESCENCE CORRELATION SPECTROSCOPY

$11,562P41FY2000RRNIH

University Of Illinois Urbana-Champaign, Champaign IL

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Abstract

Phospholipase (PLA,) from crotalus atrox venom is a homodimeric protein. The PLA, intrinsic trytophan residue and conjuated fluorophores are being examined using fluorescence, steady-state, and time-resolved, circular diochroism, and gel chromatography. The stablizing of the dimer is being perturbed using denaturants, pressure, and temperature, and monitored using the above techniques.

View original record on NIH RePORTER →