SINGLE MOLECULE DETECTION OF PROTEIN REACTIONS

$20,404P41FY2000RRNIH

University Of Illinois Urbana-Champaign, Champaign IL

Investigators

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Abstract

Single tryptophan mutants of adenylate kinase are being constructed using site-directed mutagenisis techniques. The local region of the tryptophan will be monitored as the protein conformation is perturbed with pressure, denaturant and temperature. In addition, extrinsically labeled adnylatic kinase will also be studied for similar structure/conformational information

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