DECAY MODE OF MEMBRANE PROBES DPH &[ AURDAN

$7,632P41FY2000RRNIH

University Of Illinois Urbana-Champaign, Champaign IL

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Abstract

a)TRYPTOPHAN FLUORESCENCE IN PHOSPHOFRUCTOKINASE. The temperature dependence of the frequency response of the intrinsic tryptophan emission of phosphofructokinase was studied as a function of emission wavelength. Differential phase measurements were also performed. b) FREQUENCY DOMAIN BACKGROUND SUBTRACTION: Several systems were evaluated for testing the recently developed background subtraction routines. These procedures were successfully implemented for lifetime and dynamic polarization measurements. c)2-photon FCS of FITC-labaled PFK from B. stearothermophilus

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