SALT DEPENDENCE OF PROCESSIVITY OF T45G RIBONUCLEASE

$7,533P41FY2000RRNIH

University Of Wisconsin Madison, Madison WI

Investigators

Linked publications & trials

Paper 39713312 Paper 39019798 Paper 38733345 Paper 35451368 Paper 35181664 Paper 35084962 Paper 34937906 Paper 34686998 Paper 34288305 Paper 34157720 Paper 33931701 Paper 33782120 Paper 33755420 Paper 33428379 Paper 33420032 Paper 33373180 Paper 33337146 Paper 33231432 Paper 33219414 Paper 33214279 Paper 32954361 Paper 32617786 Paper 32358061 Paper 31819058 Paper 31616394 Paper 31606082 Paper 31573406 Paper 31488839 Paper 31479276 Paper 31451767 Paper 31400167 Paper 31393705 Paper 31392081 Paper 31378069 Paper 31363014 Paper 31331976 Paper 31301804 Paper 31296583 Paper 31209011 Paper 31158365 Paper 31142651 Paper 31091298 Paper 31066162 Paper 30927511 Paper 30831325 Paper 30830436 Paper 30287687 Paper 30282936 Paper 30003682 Paper 29855542 Paper 29786646 Paper 29615504 Paper 29283511 Paper 29222330 Paper 29104816 Paper 28952732 Paper 28887770 Paper 28840714 Paper 28656061 Paper 28509413 Paper 28440746 Paper 28082426 Paper 28059082 Paper 27905089 Paper 27811964 Paper 27539579 Paper 27382050 Paper 27281824 Paper 26798564 Paper 26744790 Paper 26630444 Paper 26621745 Paper 26443865 Paper 26425937 Paper 26366246 Paper 26331259 Paper 26289554 Paper 26273993 Paper 26195753 Paper 25839233 Paper 25825269 Paper 25762536 Paper 25650931 Paper 25642355 Paper 25642011 Paper 25639645 Paper 25594737 Paper 25588614 Paper 25486224 Paper 25450980 Paper 25372495 Paper 25331866 Paper 25224746 Paper 25197007 Paper 25158017 Paper 25132967 Paper 25096878 Paper 25086049 Paper 25080945 Paper 25055301

Abstract

The salt dependence of processivity of the Threonine 45 to Glycine (T45G) mutation of Ribonuclease A will be determined using a 31P NMR based assay for processivity of T45G acting on Polyadenosine (PolyA) [S.B. delCardayr R.T. Raines, Biochemistry 33, 6031-6037 (1994)]. The NMR assay allows for the direct determination of the processivity parameter, PI, of T45G at a variety of salt conditions. From the changes in PI with the changes in salt concentration, one can assess the importance of the coulombic interactions between the positively charged basic residues on T45G with the negatively charged phosphates on PolyA on processivity in this system.

View original record on NIH RePORTER →