POST TRANSLATIONAL MODIFICATIONS OF RECOMBINANT HUMAN FACTOR VIII

$9,880P41FY2000RRNIH

University Of California San Francisco, San Francisco CA

Investigators

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Abstract

Post-translational modifications of recombinant human factor VIII will be determined. Both the "native" and reduced/aldylated "subunits" of factor VIII will be digested withtryptin and analyzed by LC/ESIMS and SIM for carbohydrates, phosphate and sulfate esters. The disulfide bridges will be determined from LC/ESIMS data of the unreduced and tryptic digest. The disulfide-containing peptides will be isolated and subjected to MALDI-TOF analysis before and after reduction.

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