SYNTHESIS OF FLUORESCENT PROBES FOR CELL STUDIES

$9,880P41FY2000RRNIH

University Of California San Francisco, San Francisco CA

Investigators

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Abstract

We have developed novel optical techniques to study solute dynamics in living cells and the kinetics of vesicular trafficking. Our laboratory has a full-time organic chemist who synthesizes specific fluorescent indicators for measurement of pH, chloride and solute dynamics in cytoplasm and certain subcellular compartments including and nucleus and endosomal secretary compartments. We utilize the mass spectrometry facility for confirmation of the structure of synthetic fluorescent probes.

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