MOUSE CHROMOSOME Y ANALYSIS &SORTING

$15,323P41FY2000RRNIH

University Of Calif-Los Alamos Nat Lab, Los Alamos NM

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Abstract

IL-2 stimulated mouse spleen cells were used as a source of mouse chromosomes for analysis and sorting of the C57BL/6J mouse Y chromosome. The DNA content of this mouse Y chromosome is approximately 94.7 megabases, making it similar in size to human chromosome 16, and significantly larger than previous estimates. Two small insert Y chromosome-specific libraries, ideal for sampling Y chromosome sequences were constructed. Clone specificity was confirmed. From the characterized clones were developed 15 male-specific PCR assays, and 19 male-female PCR assays likely to originate from the pseudoautosomal region or other areas of X-Y or autosome-Y homology. (Genomics, 48, 304-313, 1998)

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