PLASMA GLYCEROL SPECIFIC ACTIVITY BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

$6,028P41FY2000RRNIH

Washington University, Saint Louis MO

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Abstract

Previous methods for measuring plasma glycerol specific activity (SA) are suboptimal, making the determinatin of glycerol kinetics in vivo with radiotracers difficult. A new high performance liquid chromatography (HPLC) method is described which permits the accurate and specific measurement of glycerol SA. The method involves isolation of glycerol from plasma and the formation of a tribenzoyl derivative. Glycerol rate of appearance was measured in five human volunteers using both [2-3H] glycerol and [2H5] glycerol. There was close agreement between the glycerol appearance rates measured using the two approaches (1.66(0.14 vs 1.70(0.10 (mol.kg-1.min-1, respectively, P=NS). This HPLC method offers improved specificity over existing methods of measuring glycerol turnover using radiotracers.

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