CORE--TISSUE CULTURE

$207,197P01FY2000AGNIH

Scripps Research Institute, La Jolla CA

Investigators

Linked publications & trials

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Abstract

The tissue core will process human articular cartilage and isolate chondrocytes. Cells will be stimulated in high density primary culture or in alginate beads and provided to the projects for experiments. Protein and mRNA extracts will be prepared from these specimens. The studies proposed in all research components depend on these samples and this core is thus essential to the success of the program. The specific functions of the core are: 1) Isolation of chondrocytes and perichondrial cells. 2) Expansion of a cell bank with chondrocytes from different age groups. 3) Chondrocyte cultures in monolyers and alginate. 4) Preparation of cells, conditioned media, protein and nucleic acid extracts from different culture systems and stimulation conditions.

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