EFFECT OF INSULIN ON LUTEINIZING HORMONE (LH) RELEASE IN WOMEN

$575M01FY2000RRNIH

Massachusetts General Hospital, Boston MA

Investigators

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Abstract

Subjects will be admitted to the GCRC within the first 3 days after the onset of menses, or at random in amenorrhcic subjects. After an overnight fast, they will undergo 24 hours of q 10 minute blood sampling (2-3 cc per sample) for LH glucose and insulin levels. Serum pools will be tested for FSH and sex steroids. During this stay, meals will be standardized at 0830, 1200, 1500, 1800, and 2230 hrs., with calories adjusted to maintain body weight. Diazoxide 100 mg po q 8 hr will be initiated for 4 days. They will be readmitted after an overnight fast for repeat sampling under the identical protocol. Analysis: Paired t-testing within and unpaired t-testing between groups.

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