ANIMAL PROTEINS PROVIDE ALL ESSENTIAL AMINO ACIDS THAT ARE INVOLVED IN HUMAN METABOLISM, IMMUNE SYSTEM, AND FUNCTIONAL PROTEINS SYNTHESIS. THE GOAL OF THIS PROJECT IS TO DEVELOP NOVEL TECHNOLOGIES FOR REGENERATIVE ANIMAL PROTEINS (RAPS) PRODUCTION BY UTILIZING ANIMAL MEAT WASTE FROM ABATTOIRS. THE SPECIFIC OBJECTIVES AND APPROACHES OF THIS TRANSFER PROJECT ARE TO 1) MAINTAIN AND EXPANSION SELF-RENEWABLE PORCINE INDUCED PLURIPOTENT STEM CELLS (IPSCS) ; 2) DEVELOP LOW-COST SCALABLE PLANT PROTEIN-BASED HYDROGEL FOR APPLICABLE THREE-DIMENSIONAL (3D) SCAFFOLDING FOR PORCINE IPSCS; 3) OPTIMIZE 3D BIOREACTOR MANUFACTURING AND DEMONSTRATE RAP PRODUCTION FROM PORCINE SELF-RENEWABLE STEM CELLS BY PHYSICAL ENGINEERING APPROACHES AT KILO GRAM SCALE AND CONDUCT TECHNO-ECONOMIC ANALYSIS; AND 4) ASSESS NUTRITIONAL QUALITY AND PROTEIN EFFICIENCY AND PROTEIN DIGESTIBILITY OF THE RAPS IN VITRO AND IN VIVO RAT MODEL. THIS TRANSFER PROJECT ALIGNS WELL WITH NIFA'S OVERALL GOALS OUTLINED IN AFRI A1364 NOVEL FOODS AND INNOVATIVE MANUFACTURING TECHNOLOGIES AND THE GOAL OF THIS RFA FY 2025 10.310. THIS RESEARCH WILL GENERATE NEW KNOWLEDGE AND DEVELOP COST-EFFECTIVE 3D BIOMANUFACTURING TECHNOLOGIES FOR HIGH-EFFICIENT PRODUCTION OF RAPS UTILIZING ANIMAL WASTE IN ABATTOIRS FOR HIGH-QUALITY DROP-IN INNOVATIVE FOOD PROTEINS. THE INTEGRATED NOVEL 3D BIOMANUFACTURING TECHNOLOGIES WILL OPEN A NEW DOOR FOR INNOVATIVE FOOD PROTEINS, WHICH WOULD INCREASE JOB REPLACEMENT IN THE RELATED AREA AND IMPROVE PRODUCER RETURN AND LONG-TERM SUSTAINABILITY FOR FOOD SECURITY, AND ULTIMATELY ENHANCE THE ECONOMIC VIABILITY OF MEAT INDUSTRIES, FOOD INDUSTRIES, AND AGRICULTURAL RELATED INDUSTRIES.