** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** TURKEY MEAT IS THE SECOND MOST POPULAR POULTRY MEAT IN THE WORLD AND THE U.S. IS THE BIGGEST PRODUCER AND EXPORTER. BY IMPROVING PRODUCTION EFFICIENCY, THE TURKEY INDUSTRY CAN HELP ACHIEVE FOOD SECURITY. ONE MAJOR ISSUE IS THE DECLINE IN FERTILITY OF PARENT FLOCKS, WHICH MAKES IT HARD TO SUPPLY COMMERCIAL POULTS TO TURKEY GROWERS. CURRENTLY, THERE IS NO EFFECTIVE WAY TO STOP OR REVERSE THIS DECLINE. THIS STUDY AIMS TO UNDERSTAND THE CHANGES IN THE FEMALE REPRODUCTIVE SYSTEM THAT CAUSE FERTILITY DECLINE AND IF USING ANTI-INFLAMMATORY TREATMENTS CAN HELP PREVENT OR RESTORE FERTILITY. IN THE FIRST EXPERIMENT, THE ROLES OF EACH CELL IN THE REPRODUCTIVE SYSTEM BEFORE AND AFTER FERTILITY DECLINE, AS WELL AS THE COMMUNICATION BETWEEN THESE CELLS, WILL BE EXAMINED. DISTURBANCES IN THE MICROBIAL POPULATION OF THE REPRODUCTIVE TRACT WILL ALSO BE ANALYZED TO IDENTIFY HARMFUL MICROBES THAT EXACERBATE INFLAMMATION AND CAUSE FERTILITY LOSS. IN THE SECOND EXPERIMENT, AN ANTI-INFLAMMATORY COMPOUND WILL BE TESTED TO SEE IF IT CAN PREVENT OR IMPROVE FERTILITY. IF THIS COMPOUND WORKS, OUR FUTURE EFFORTS WILL FOCUS ON ITS FURTHER DEVELOPMENT AND IMPLEMENTATION IN THE INDUSTRY. INFORMATION GENERATED FROM THIS STUDY WILL INCLUDE THE DISCOVERY OF POTENTIAL MARKERS OF HEN FERTILITY AS WELL AS STRATEGIES TO PREVENT AND/OR IMPROVE FERTILITY LOSS. THE FINDINGS FROM THIS STUDY WILL BENEFIT THE TURKEY INDUSTRY AS WELL AS OTHER POULTRY INDUSTRIES AND ALLOW FOR INCREASED PRODUCTION WHILE CONSERVING RESOURCES.