PRUNES (PRUNUS DOMESTICA L.) SERVE AS A HIGHLY EFFECTIVE WHOLE FOOD DIET-BASED MEANS TO BOTH PREVENT AND REVERSE BONE LOSS WITH MINIMAL SIDE EFFECTS IN MALES, FEMALESAND NUMEROUS ANIMAL MODELS. HOWEVER, NO STUDIES EXIST EVALUATING THEIR IMPACTS ON BONE AND OTHER HEALTH OUTCOMES IN PERIMENOPAUSAL FEMALES, WHICH NECESSITATES A LONGER-TERM INTERVENTION THAN OUR PREVIOUS STUDIES. FURTHERMORE, THE CURRENTLY KNOWN CLINICALLY EFFECTIVE DOSE OF PRUNES IS 50 G/D, WHICH IS RELATIVELY HIGH AND LIMITS DIETARY ADHERENCE AND COMPLIANCE. OUR GOALS ARE TO ASSESS THE LONG-TERM (2 YEARS) IMPACT OF PRUNES FED AT A PROVEN DOSE (50 G/D) SERVING AS A POSTIVE CONTROL AND AT A LOWER, MORE FEASIBLE LEVEL FOR DAILY CONSUMPTION OF 30 G/D ON BONE HEALTH AND OTHER HEALTH OUTCOMES. WITHIN OUR 2-YEAR INTERVENTION, WE WILL ALSO ASSESS MULTIPLE POTENTIAL MECHANISMS OF ACTION FOR IMPROVEMENTS IN BONE INCLUDING IMPACTS ON CALCIUM ABSORPTION AND MOLECULAR TRANSDUCERS OF BONE PHYSIOLOGY USING MULTIPLE TECHNIQUES. FURTHERMORE, WE WILL ASSESS SHORTER-TERM (28 DAYS) AND ACUTE (2 HOURS) EFFECTS OF PRUNES ON NUMEROUS HEALTH OUTCOMES. OUR FINDINGS WILL PROVIDE DATA CRITICAL TO THE UNDERSTANDING OF THE EFFECTS OF PRUNES IN THIS CRITICAL LIFE PERIOD FOR FEMALES.