COPROPHAGOUS FLIES ARE UNIQUELY SUITED TO BE USED AS ONE HEALTH BIOMONITORS OF TERRESTRIAL ANTIMICROBIAL RESISTANCE. WHERE MULTIPLE SOURCES OF FECAL MATERIAL ARE ACCESSIBLE TO THEM, COPROPHAGOUS FLIES ACT AS COMPOSITE SAMPLERS OF LOCALIZED TERRESTRIAL AMR BY REPEATEDLY FEEDING ON UNCONTAINED WASTES. IN AGRICULTURAL SETTINGS, FLIES ALSO POSE A RISK OF TRANSPORTING ARBS AND ARGS FROM FECAL WASTES TO AGRICULTURAL PRODUCTS. WE PROPOSE FURTHER STUDY OF COPROPHAGOUS FLIES TO BETTER UNDERSTAND THE PERSISTENCE AND EMISSION OF ARB AND ARGS AS PART OF A NOVEL APPROACH TO MONITOR AND MITIGATE AMR IN TERRESTRIAL ENVIRONMENTS. OUR THREE RESEARCH OBJECTIVES INCLUDE, (1) DETERMINE THE RATE OF INGESTION AND PERSISTENCE OF ARB AND ARGS IN COPROPHAGOUS FLIES UNDER A RANGE OF ENVIRONMENTAL CONDITIONS; (2) DETERMINE THE EXCRETION OF ARB AND ARGS FROM COPROPHAGOUS FLIES TO SURFACES UNDER A RANGE OF ENVIRONMENTAL CONDITIONS; AND (3) DETERMINE THE RELATIONSHIP BETWEEN ARB AND ARGS IN WILD CAUGHT COPROPHAGOUS FLIES WITH TEMPORALLY AND GEOGRAPHICALLY MATCHED AGRICULTURAL PRODUCTS AND IRRIGATION WATER. WE WILL CONDUCT CONTROLLED FEEDING STUDIES USING COPROPHAGOUS FLIES TO ACCOMPLISH OBJECTIVES 1 AND 2. WE WILL INCORPORATE OBJECTIVE 3 INTO OUR ONGOING FIELD WORK IN MAPUTO, MOZAMBIQUE. THIS FUNDAMENTAL WORK ADVANCES OUR UNDERSTANDING HOW COPROPHAGOUS FLIES CAN BE USED AS ONE HEALTH BIOMONITORS OF TERRESTRIAL ANTIMICROBIAL RESISTANCE. THE VALUABLE INSIGHTS INTO THE SPREAD AND PREVALENCE OF AMR, WILL ENABLE TARGETED INTERVENTIONS TO REDUCE ITS IMPACT IN THE FOOD CHAIN AND HELP BREAK THE TRANSMISSION CYCLE OF RESISTANT BACTERIA, IMPROVING PUBLIC HEALTH.