**AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** MODERN AGRICULTURE RELIES HEAVILY ON THE USE OF PESTICIDES TO PROTECT CROPS. IN RESPONSE TO SOCIETY'S NEED FOR SAFER AND MORE ENVIRONMENTALLY FRIENDLY ALTERNATIVES TO SYNTHETIC PESTICIDES, WE HAVE DESIGNED THE PILOT PLANT FOR PRODUCTION OF BIOPESTICIDAL EXTRACTS FROM MUSTARD SEED FROM WHICH THE OIL HAS BEEN REMOVED AS A FEEDSTOCK FOR BIOFUEL. BECAUSE THE SUCCESS IN THE FORM OF GROWER ADOPTION REQUIRES CONSISTENT AND ECONOMICALLY VIABLE PRODUCT, THE OVERALL GOAL OF THE PROJECT IS TO PROVIDE A BIOPESTICIDES FORMULATION THAT CAN BE GROWER-IMPLEMENTED. TO INCREASE THE EXTRACT VALUE AND THE QUALITY OF BIOPESTICIDES, WE PROPOSE TO DESIGN AND OPTIMIZE AN ULTRAFILTRATION SYSTEM THAT WOULD ALLOW US TO SEPARATE MULTIPLE BIOLOGICALLY ACTIVE PRODUCTS SUCH AS ENZYMES FROM MUSTARD EXTRACTS. WE WILL 1) PRODUCE WATER-BASED PESTICIDAL EXTRACTS FROM YELLOW AND ORIENTAL MUSTARDS; 2) DESIGN ULTRAFILTRATION SYSTEM FOR MYROSINASE ENZYME SEPARATION FROM MUSTARD EXTRACT; 3) OPTIMIZE DRYING TECHNIQUE FOR SHELF-STABILIZATION OF ENZYME; AND 4) ASSESS THE EFFICIENCY OF THE PRODUCED BIOPESTICIDE FORMULATIONS. BY PRODUCING MULTIPLE HIGH VALUE PRODUCTS FROM MUSTARD EXTRACT WE WILL PROVIDE INSENSITIVE FOR GROWING MUSTARD, A GREAT ROTATIONAL AND COVER CROP THAT CAN BE USED TOIMPROVE SOIL HEALTH AND REDUCE PEST PRESSURE.