** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** AFRICAN SWINE FEVER (ASF) IS A HIGHLY CONTAGIOUS VIRAL DISEASE WITH MORTALITY UP TO 100% IN DOMESTIC SWINE HERDS. ASF OUTBREAKS IN EURASIA HAVE RESULTED IN TREMENDOUS ECONOMIC LOSSES TO THOSE AFFECTED COUNTRIES AND THE INTRODUCTION OF ASF INTO US SWINE INDUSTRY WOULD LEAD TO APPROXIMATELY $50 BILLION IN LOSSES. ALTHOUGH LIVE ATTENUATED VACCINES HAVE ACHIEVED PARTIAL SUCCESS, SAFETY CONCERN AND VIRAL PERSISTENCE ARE THE MAJOR ISSUES. IN THIS PROJECT, RATIONAL DESIGN AND EVALUATION OF A ASF MRNA VACCINE WILL BE CONDUCTED. THE GOAL OF THIS STUDY IS TO EVALUATE THE ENGINEERED VACCINE ANTIGENS USING LNP-MRNA FORMULATIONS FOR STIMULATION OF STRONG ANTIBODY AND T CELL IMMUNE RESPONSES.THIS WORK WILL ULTIMATELY LEAD TO DEVELOPMENT OF A MRNA VACCINE AGAINST ASFIN AID OF DISEASE CONTROL AND SWINE HEALTH.