** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** CLONING IS AN IMPORTANT TOOL IN MANY RESEARCH FIELDS AND HOLDS GREAT PROMISE FOR THE CREATION OF DOMESTIC ANIMALS WITH HIGH GENETIC VALUE. HOWEVER, THE ANIMAL PRODUCTION EFFICIENCY OF CLONING REMAINS LOW. EGG QUALITY PLAYS A CRITICAL ROLE IN THE EFFICIENCY OF EMBRYO AND FETUS DEVELOPMENT. A SIGNIFICANT DETERMINANT OF EGG QUALITY IS THE ABILITY TO POLYADENYLATE GENETIC MESSAGE TO ALLOW EGG DEVELOPMENT. POLYADENYLATION IS THE PROCESS OF ADDING A PROTECTIVE AND TRANSPORT-FRIENDLY TAIL ON THE END OF THE GENETIC MESSAGE, STABILIZING IT SO THAT CAN BE EFFICIENTLY READ BY THE CELL'S PROTEIN-MAKING MACHINERY. OUR WORKING HYPOTHESIS IS THAT LOW QUALITY EGGS INEFFICIENTLY POLYADENYLATE GENETIC MESSAGES AND THAT INCREASING EGG POLYADENYLATION WILL IMPROVE EGG QUALITY AND THE EFFICIENCY OF CLONING. WE WILL TEST THIS HYPOTHESIS BY ADDING EXTRA PROTEIN, WHICH CONTROLS THE POLYADENYLATION PROCESS (CPEB1), IN CATTLE CLONED EGGS AND EVALUATE THE EFFECTS ON EMBRYO QUALITY AND GENE EXPRESSION IN CLONED EMBRYOS. WITH DISCOVERIES MADE IN THIS WORK ON THE ROLE OF POLYADENYLATION DURING CLONED EMBRYO DEVELOPMENT, WE HAVE GREAT POTENTIAL TO CREATE A NEW APPROACH TO IMPROVE OOCYTE QUALITY AND DEVELOPMENT OF CLONED EMBRYOS,LEADING TO IMPROVEMENTS IN CATTLE CLONING THAT WILL INCREASE PRODUCTION EFFICIENCY.