** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** THE PLANT-BASED DAIRY AND MEAT MARKET IS BOOMING, ESTIMATED TO BE WORTH $29 BILLION IN 2023 AND GROWING BY ABOUT 8% ANNUALLY. THIS REFLECTS THE RISING GLOBAL DEMAND FOR ALTERNATIVE PROTEINS, INCLUDING VARIOUS PLANT-BASED SOURCES. SCIENTISTS ARE NOW FOCUSING ON DEVELOPING NEW INGREDIENTS FROM CEREALS, PULSES, AND MICROBES, WITH IMPROVED FUNCTIONAL PROPERTIES. HOWEVER, TRADITIONAL PROTEIN EXTRACTION METHODS, WHICH INVOLVE ALKALINE PH AND ISOELECTRIC PRECIPITATION, OFTEN RESULT IN LOWER YIELDS AND REDUCED PROTEIN QUALITY IN TERMS OF FUNCTIONALITY, TASTE, AND NUTRITION. THE TRADITIONAL METHOD ALSO REQUIRES LARGE AMOUNTS OF CHEMICALS, WHICH IS NOT SUSTAINABLE. THIS PROJECT AIMS TO DEVELOP AN ECO-FRIENDLY METHOD FOR FRACTIONATING PLANT PROTEINS TO ENHANCE CONSUMER ACCEPTANCE BY IMPROVING FUNCTIONALITIES, TASTE, AND NUTRITIONAL VALUE. IT WILL UTILIZE ULTRASOUND AND FERMENTATION TECHNOLOGIES TO EXTRACT PROTEINS FROM PULSE FLOUR, INCREASING YIELD AND IMPROVING PROTEIN QUALITY. THE RESEARCH WILL FOCUS ON OPTIMIZING ULTRASOUND-ASSISTED EXTRACTION TO RELEASE PROTEINS FROM FLOUR EFFICIENTLY, FOLLOWED BY ADJUSTING FERMENTATION-ASSISTED PRECIPITATION TO REMOVE OFF-FLAVORS AND ANTINUTRITIONAL FACTORS. THE EFFECTIVENESS OF THE NEW METHOD WILL BE COMPARED WITH TRADITIONAL EXTRACTION METHODS IN TERMS OF PROTEIN YIELD, REMOVAL OF OFF-NOTES, AND REDUCTION OF ANTINUTRITIONAL COMPOUNDS. THE SUCCESSFUL COMPLETION OF THIS PROJECT IS EXPECTED TO RESULT IN A NEW METHOD FOR FRACTIONATING PROTEINS THAT ADDRESSES ALL ISSUES RELATED TO PULSE PROTEIN. THE METHOD WOULD HAVE A LOWER ENVIRONMENTAL IMPACT, POTENTIALLY REDUCING EMISSIONS USING LESS LAND AND WATER THAN TRADITIONAL MEAT. THEREFORE, THIS PROJECT COULD PLAY A CRUCIAL ROLE IN ENSURING THE LONG-TERM SUSTAINABILITY OF U.S. AGRICULTURE AND FOOD SYSTEMS.