** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** HOP LATENT VIROID (HLVD) IS AN EMERGING PATHOGEN AFFECTING HEMP WITH POTENTIAL TO CAUSE SIGNIFICANT DAMAGE AND CROP LOSS. SINCE ITS INITIAL DETECTION IN CALIFORNIA IN 2019, HLVD HAS BEEN DETECTED IN ALL MAJOR HEMP GROWING STATES INCLUDING COLORADO, OREGON, WASHINGTON AND IN CANADA. THROUGH THIS GRANT PROPOSAL, PDS AT COLORADO STATE UNIVERSITY AND COLLABORATOR AT SIMON FRASER UNIVERSITY IN CANADA WILL ENGAGE INTERNATIONALLY TO DEVELOP AND APPLY NEW KNOWLEDGE AND PRACTICES REGARDING HLVD TO MITIGATE DISEASE IMPACT.THE GOALS OF THIS CARE PROPOSAL WILL BE ACCOMPLISHED BY THREE OBJECTIVES:1. DETERMINE MODES OF HLVD TRANSMISSION BY ARTHROPOD VECTORS, POLLEN, AND SEED.2. DETERMINE THE EFFECT OF INDUCED CHEMICAL RESISTANCE IN REDUCING HLVD SYMPTOM DEVELOPMENT AND INFECTION LEVELS.3. DEVELOP EFFECTIVE METHODS TO PROVIDE RESEARCH-BASED INFORMATION TO HEMP GROWERS AND OTHER STAKEHOLDERS.THESE OBJECTIVES WILL BE ADDRESSED THROUGH LABORATORY AND GREENHOUSE EXPERIMENTS IN COLORADO, WHEREAS THE INTERNATIONAL COLLABORATOR WILL SUBMIT A SEPARATE GRANT APPLICATION TO NATURAL SCIENCES AND ENGINEERING RESEARCH COUNCIL OF CANADA TO INVESTIGATE RELATED ASPECTS OF HLVD BIOLOGY AND MANAGEMENT IN ADDITION TO THE CURRENT PROPOSAL. THE RESEARCH WE PROPOSE ADDRESSES PRIORITY AREA, A) PLANT HEALTH AND PRODUCTION AND PLANT PRODUCTS WHICH HIGHLIGHTS BREAK-THROUGH DISCOVERIES AND THE TRANSLATION OF THESE INTO PLANT PRODUCTION AND PROTECTION PRACTICES. AN INTEGRATIVE (RESEARCH AND EXTENSION) APPROACH WILL TRAIN THE NEXT GENERATION OF SCIENTISTS AND NEW TOOLS WILL BE MADE READILY AVAILABLE TO STAKEHOLDERS AND PUT INTO PRACTICE.