** AWARDS ISSUED PRIOR TO JANUARY 20, 2025, WERE FUNDED UNDER PREVIOUS ADMINISTRATIONS AND MAY NOT REFLECT THE PRIORITIES AND POLICIES OF THE CURRENT ADMINISTRATION.** U.S. DOE IDENTIFIED ENERGYCANE (SACCHARUM SPP HYBRID) AS IDEAL BIOFUEL CROP FOR THE SOUTHEAST. THIS PROJECT WILL COMPARE ON AND OFF-TARGET EFFECTS OF ALTERNATIVE BIOTECHNOLOGY APPROACHES FOR COMPLETE SUPPRESSION OF FLOWERING IN ENERGYCANE. THIS WILL ELEVATE BIOCONTAINMENT BY PREVENTING GENE FLOW TO SEXUALLY COMPATIBLE WEEDY RELATIVES AND INFORM REGULATORS ABOUT THE PRECISION AND EFFECTIVENESS OF THE ALTERNATIVE APPROACHES.